Hankali da ƙayyadaddun bayanai: An kwatanta na'urar gwajin gaggawa ta 2019-nCOV/COVID-19 IgG/IgM da babbar kasuwa RT-Gwajin PCR ta amfani da samfurori na asibiti.Sakamakon ya nuna cewa 2019-nCoV/COVID-19 IgG/IgM Gwajin Saurin Na'urar yana da hazaka da ƙayyadaddun bayanai.

Don gwajin IgG:

Hankalin Dangi:233/268=86.94%(95%CI*:82.35%-90.49%)

Ƙimar Dangi:287/289=99.31% (95%CI*:97.52%-99.92%)

Daidaito: 520/557=93.36% (95% CI*: 90.96%-95.16%)

*Tazarar Amincewa

Don gwajin IgM

Hankalin Dangi:223/268=83.21% (95%CI*:78.19%-87.48%)

Ƙimar Dangi:282/289=97.58% (95%CI*: 95.07%-99.02%)

Daidaito: 505/557=90.66% (95%CI*: 87.94%-92.95%)

*Tazarar Amincewa

2019-nCOV/COVID-19 IgG/IgM Na'urar Gwajin Sauri shine saurin chromatographic immunoassay don gano ƙimar IgG&IgM antibody na Coronavirus Disease 2019 a cikin jinin ɗan adam gaba ɗaya, jini, ko plasma azaman taimako a cikin gano cututtukan COVID - 19.

NUFIN AMFANI: The 2019-nCOV/COVID-19 IgG/IgM Na'urar Gwajin Sauri shine saurin rigakafin ƙwayoyin cuta na chromatographic don gano ƙimar IgG&IgM antibody na Coronavirus Disease 2019 a cikin jinin ɗan adam gaba ɗaya, jini, ko plasma azaman taimako a cikin gano cututtukan COVID - 19.

TAKAYYA: COVID-19 cuta ce mai saurin kamuwa da numfashi. Mutane gabaɗaya suna da sauƙi. A halin yanzu, marasa lafiya da suka kamu da cutar sankara ta coronavirus sune babban tushen kamuwa da cuta; Mutanen da suka kamu da asymptomatic suma suna iya zama tushen kamuwa da cuta. Dangane da binciken cututtukan cututtuka na yanzu, lokacin shiryawa shine kwanaki 1 zuwa 14, galibi daga kwanaki 3 zuwa 7. Babban bayyanar cututtuka sun haɗa da zazzabi, gajiya da bushewar tari. Ana samun cunkoso na hanci, hanci, ciwon makogwaro, myalgia da gudawa a wasu lokuta.

HANYOYIN AMFANI: Bada na'urar gwaji, samfuri, buffer, da/ko sarrafawa don isa zafin ɗaki (15-30°C) kafin gwaji. 1. Kawo jakar zuwa zafin jiki kafin buɗewa. Cire na'urar gwajin daga jakar da aka rufe kuma yi amfani da ita da wuri-wuri. 2. Sanya na'urar gwajin akan wuri mai tsabta da matakin. ? Don samfuran Serum ko Plasma: Yin amfani da pipette ɗin da aka bayar na 10μL, zana samfurin har zuwa Layin Cika, kuma canja wurin 10μL serum/plasma zuwa samfurin rijiyar na'urar gwajin, sannan ƙara digo 2 na buffer kuma fara mai ƙidayar lokaci. ? Ga Dukkan Jini (Venipuncture/Fingerstick) Samfura: Yin amfani da pipette na 10μL da aka bayar, da kuma canja wurin digo 1 na duka jini (kimanin 20μL) zuwa samfurin da kyau na na'urar gwajin, sannan ƙara digo 2 na buffer kuma fara mai ƙidayar lokaci. Lura: Hakanan ana iya amfani da samfurori ta amfani da micropipette. 3. Jira layin (s) masu launi ya bayyana. Karanta sakamakon a minti 10. Kada ka fassara sakamakon bayan minti 15.

KA'IDA: Wannan kit ɗin yana amfani da immunochromatography.Katin gwajin ya ƙunshi:1) zinare mai launi Layin M ba shi da motsi tare da maganin rigakafi na IgM na ɗan adam don gano wani sabon ƙwayar cuta na IgM na coronavirus; Layin IgG ba shi da motsi tare da reagent don gano sabon ƙwayar cuta ta IgG na coronavirus; kuma layin C ba shi da motsi tare da antibody mai sarrafa inganci. Lokacin da aka ƙara adadin da ya dace na samfurin gwajin zuwa ramin samfurin katin gwajin, samfurin zai ci gaba tare da katin gwajin a ƙarƙashin aikin capillary. Idan samfurin ya ƙunshi antibody IgM, antibody zai ɗaure zuwa colloidal zinariya-labeled novel coronavirus antigen. Za a kama hadadden na rigakafi ta anti - ɗan adam IgM antibody wanda ba zai iya motsi a jikin membrane don samar da layin jan m mai ruwan hoda, yana nuna cewa sabon coronavirus IgM antibody yana da inganci. Idan samfurin ya ƙunshi maganin rigakafi na IgG, antibody ɗin zai ɗaure da zinare na colloidal - wanda aka yiwa lakabi da coronavirus antigen, kuma rukunin rigakafi zai kama shi ta hanyar reagent immobilized akan membrane don samar da layin IgG mai shunayya, yana nuna cewa sabon coronavirus IgG antibody yana da inganci. Idan gwajin IgG da layin IgM ba su da launin launi, ana nuna mummunan sakamako.Katin gwajin kuma ya ƙunshi layin kula da inganci C. Ya kamata layin kula da ingancin fuchsia C ya bayyana ba tare da la'akari da ko layin gwajin ya bayyana ba. Idan layin sarrafa ingancin C bai bayyana ba, sakamakon gwajin ba shi da inganci, kuma samfurin yana buƙatar sake gwadawa tare da wani katin gwaji.