Atụle uche na nkọwapụta: Ihe 2019-nCOV/COVID-19 IgG/IgM Ngwa ule ngwa ngwa ka atụnyere nleba anya azụmahịa RT-Nnwale PCR site na iji ụdị ụlọ ọgwụ. Nsonaazụ gosiri na 2019-nCoV/COVID-19 IgG/IgM Ngwa ule ngwa ngwa nwere ọgụgụ isi na nkọwapụta.

Maka ule IgG:

Mmetụta mmetụta:233/268=86.94%(95%CI*:82.35%-90.49%)

Nkọwa nkọwa:287/289=99.31% (95%CI*:97.52%-99.92%)

Nzizi:520/557=93.36% (95%CI*: 90.96%-95.16%)

* Ogologo oge ntụkwasị obi

Maka ule IgM

Mmetụta mmetụta:223/268=83.21% (95%CI*:78.19%-87.48%)

Nkọwa nkọwa:282/289=97.58% (95%CI*: 95.07%-99.02%)

Nzizi: 505/557=90.66% (95%CI*: 87.94%-92.95%)

* Ogologo oge ntụkwasị obi

Ụkpụrụ: Ngwa a na-eji immunochromatography. The test card nwere:1) colloidal gold-akpọrọ recombinant novel coronavirus antigen na quality control antibody gold markers;2) ahịrị nchọpụta abụọ (IgG na IgM ahịrị) na otu njikwa njikwa (C ahịrị) nke akpụkpọ anụ nitrocellulose. The IgM line is immobilized with a monocloid IgM line is immobilized with a monoclogMid antibody antibody. mgbochi; A na-eji reagent eme ka ahịrị IgG ghara imegharị ya maka ịchọpụta ihe mgbochi coronavirus IgG ọhụrụ; na ahịrị C na-enweghị ike imegharị ya na njikwa njikwa mma. Mgbe agbakwunyere ọnụ ọgụgụ kwesịrị ekwesị nke ihe nlele ule na oghere nlele nke kaadị ule ahụ, ihe nlele ahụ ga-aga n'ihu na kaadị ule n'okpuru ọrụ nke capillary. Ọ bụrụ na ihe nlele ahụ nwere ihe mgbochi IgM, mgbochi ahụ ga-ejikọta ya na colloidal gold - nke akpọrọ coronavirus antigen. A ga-ejide ogige ahụ na-alụso ọrịa ọgụ site na mgbochi - mmadụ IgM antibody immobilized na akpụkpọ ahụ ka ọ bụrụ ahịrị IgM na-acha odo odo, na-egosi na akwụkwọ akụkọ coronavirus IgM antibody dị mma. ahịrị, na-egosi na akwụkwọ akụkọ coronavirus IgG antibody dị mma. Ọ bụrụ na ule IgG na ahịrị IgM adịghị agba, a na-egosipụta nsonaazụ na-adịghị mma. Kaadị ule nwekwara akara njikwa mma C. The fuchsia quality akara akara C kwesịrị ịpụta n'agbanyeghị ma akara ule gosipụtara. Ọ bụrụ na ahịrị njikwa mma C egosighi, nsonaazụ nnwale ahụ abaghị uru, yana ekwesịrị iji kaadị ule ọzọ nwalee ihe nlele ọzọ.

Nke a bụ ihe ị ga-eme iji mee ule:

1.Mkpịsị aka dị ọcha na mmanya mmanya.

2.Tinye otu mkpịsị aka na mkpịsị aka iji nweta nlele ọbara.

3.Iwepu mkpuchi agịga tụgharịa na elekere elekere na-eso akụ na mkpuchi.

4.Jiri dropper isere ọbara tinye kaseti ule.

5. Lelee akara na cassette 10mins ka e mesịrị ka ịhụ nsonaazụ.

Coronavirus (CoV) bụ nke genus Nestovirus, Coronaviridae, wee kewaa ya n'ime atọ: α, β, na γ. genus α na β bụ naanị ọrịa na-ebute anụ ahụ. Ụdị γ na-ebutekarị ọrịa nnụnụ. Enwekwara ihe akaebe na enwere ike ibunye ya site n'ụzọ fecal-ọnụ ọnụ.

Ka ọ dị ugbu a, e nwere ụdị coronavirus mmadụ 7 (HCoV) na-ebute ọrịa iku ume mmadụ: HCoV-229E,HCoV-OC43,SARS-CoV,HCoV-NL63,HCoV-HKU1,MERS-CoV na novel coronaviruses (2019), bụ ihe dị mkpa pathogen nke mmadụ iku ume ọrịa. Ngosipụta bụ mgbaàmà sistemu dị ka ahụ ọkụ na ike ọgwụgwụ, na-esonyere ụkwara akọrọ na dyspnea, wdg, nke nwere ike ịmalite ngwa ngwa ghọọ nnukwu oyi baa, nkwụsị iku ume, na nnukwu iku ume. na-ọbụna ndụ-egwu.

Ngwa 2019-nCOV/COVID-19 IgG/IgM Ngwa ngwa ngwa ngwa bụ immunoassay chromatographic ngwa ngwa maka nchọpụta qualitative nke IgG&IgM antibody nke Coronavirus Ọrịa 2019 n'ime ọbara mmadụ dum, serum, ma ọ bụ plasma dị ka ihe enyemaka na nchoputa nke COVID-19 ọrịa.