【USHWANKATHELO】

I-Mycoplasma pneumoniae yindlela eqhelekileyo yokuphefumula i-pathogen eyenza i-pharyngitis, i-tracheobronchitis, okanye i-pneumonia ye-1. Kuya kubakho uphawu olunentloko ebuhlungu, ifiva, ukukhohlela okomileyo, kunye neentlungu zemisipha. Abantu bayo yonke iminyaka yobudala banokosuleleka ngelixa ulutsha, abaphakathi - ababudala kunye nabantwana abangaphantsi kweminyaka emi-4 ubudala benezinga eliphezulu losulelo. I-30% yabantu abosulelekileyo banokuba nosulelo olupheleleyo lwemiphunga. Kusulelo oluqhelekileyo, i-Mycoplasma pneumoniae IgM inokufunyanwa kwangoko kwiveki ye-1 emva kokusuleleka, iqhubeke nokunyuka ngokukhawuleza kakhulu ixesha elide, ukunyuka malunga neeveki ezi-4 ~ 5. Ukufunyaniswa kwangexesha lokusuleleka kweMycoplasma pneumoniae IgM kunokuphucula izinga lokubona usulelo lweMycoplasma pneumoniae.

【IZIKHOKELO ZOKUSETYENZISWA】

Vumela uvavanyo, umfuziselo, isithinteli kunye/okanye nezilawuli ukufikelela kubushushu begumbi (15-30°C) phambi kovavanyo.

1. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokuba usivule. Susa ikhasethi yovavanyo kwisingxobo esivaliweyo kwaye uyisebenzise ngokukhawuleza. Iziphumo ezigqwesileyo ziya kufumaneka ukuba uvavanyo lwenziwe kwiyure enye.

2. Beka ikhasethi kwindawo ecocekileyo kunye nenqanaba. Bamba idropha ngokuthe nkqo, zoba isampuli (igazi elipheleleyo/i-serum/plasma) ukuya kwindawo njengoko kubonisiwe kumfanekiso ongezantsi (malunga ne-10μl). Dlulisa umzekeliso kwisampulu kakuhle (S) nganye, emva koko ubambe ibhotile yesithinteli ngokuthe nkqo kwaye wongeze amathontsi ama-2 e-buffer (malunga ne-80μl) kwisampulu kakuhle (S), kwaye uqalise isibali-xesha. Jonga lo mzekeliso ungezantsi.

3. Lindela umgca onemibala ukuba uvele. Isiphumo kufuneka sifundwe ngemizuzu eyi-10. Sukutolika iziphumo emva kwemizuzu engama-20.