ʻIke a me ka kikoʻī: Ua hoʻohālikelike ʻia ka 2019-nCOV/COVID-19 IgG/IgM Rapid Test Device me kahi ʻoihana koʻikoʻi RT-PCR hoʻāʻo me ka hoʻohana ʻana i nā specimens.

No ka ho'āʻo IgG:

Pilikino Pili:233/268=86.94%(95%CI*:82.35%-90.49%)

ʻIkepili Pili:287/289=99.31% (95%CI*:97.52%-99.92%)

Pololei:520/557=93.36% (95%CI*: 90.96%-95.16%)

* Kūlana hilinaʻi

No ka ho'āʻo IgM

Pilikino Pili:223/268=83.21% (95%CI*:78.19%-87.48%)

ʻIkepili Pili:282/289=97.58% (95%CI*: 95.07%-99.02%)

Pono: 505/557=90.66% (95%CI*: 87.94%-92.95%)

* Kūlana hilinaʻi

PRINCIPLE: Ke hoʻohana nei kēia pahu i ka immunochromatography. Aia i loko o ke kāleka hoʻāʻo: 1) colloidal gula-labeled recombinant novel coronavirus antigen a me ka quality control antibody gold markers;2) ʻelua laina ʻike (laina IgG a me IgM) a me hoʻokahi laina Manaʻo maikaʻi (laina C) o ka membrane nitrocellulose. Hoʻopaʻa ʻia ka laina IgM me kahi antibody antibody IgM coronavirus no ke kanaka; Hoʻopili ʻia ka laina IgG me kahi reagent no ka ʻike ʻana i kahi antibody IgG coronavirus hou; a hoʻopaʻa ʻia ka laina C me kahi antibody mana maikaʻi. Ke hoʻohui ʻia ka nui kūpono o ka hāpana hoʻāʻo i ka lua hāpana o ke kāleka hoʻāʻo, e neʻe ka hāpana ma ke kāleka hōʻike ma lalo o ka hana a ka capillary. E hopu ʻia ka paʻakikī immune e ka anti-human IgM antibody i hoʻopaʻa ʻole ʻia ma ka membrane e hana i kahi laina ʻulaʻula ʻulaʻula IgM, e hōʻike ana i ka maikaʻi o ka antibody IgM coronavirus novel. maikaʻi ka antibody IgG coronavirus hou. Inā ʻaʻole kala ʻia nā laina IgG a me IgM hoʻāʻo, hōʻike ʻia kahi hopena maikaʻi ʻole. Aia pū i ka kāleka hoʻāʻo kahi laina mana maikaʻi C. Pono ka laina hoʻomalu maikaʻi fuchsia C e ʻike ʻole inā ʻike ʻia kahi laina hōʻike. Inā ʻaʻole ʻike ʻia ka laina hoʻokele maikaʻi C, ʻaʻole kūpono ka hopena hōʻike, a pono e hoʻāʻo hou ʻia ka hāpana me kahi kāleka hōʻike ʻē aʻe.

Eia ka mea e pono ai ʻoe e hana no ka hoʻokō ʻana i ka hoʻāʻo:

1. E hoʻomaʻemaʻe i ka manamana lima me ka waiʻona.

2. Puncture hookahi manamana me ka manamana lima pricker e kiʻi koko hāpana.

3. No ka hoʻokuʻu ʻana i ka uhi nila e hoʻohuli i ka uaki ma hope o nā pua ma ka uhi.

4.E hoʻohana i ka dropper e huki i ke koko e noi i ka cassette hoʻāʻo.

5. E nānā i nā kaha ma ka cassette ma hope o 10 mau minuke e ʻike i ka hopena.

ʻO Coronavirus (CoV) no ka genus Nestovirus, Coronaviridae, a ua māhele ʻia i ʻekolu genera: α, β, a me γ. ʻO ka genus α a me β he pathogenic wale nō i nā mammals. ʻO ka genus γ ke kumu nui o nā maʻi manu. Aia kekahi mau hōʻike e hiki ke lawe ʻia ma ke ala fecal-waha.

I kēia manawa, aia 7 ʻano o ka coronavirus kanaka (HCoV) e hoʻoulu ai i nā maʻi hanu kanaka: HCoV-229E,HCoV-OC43,SARS-CoV,HCoV-NL63,HCoV-HKU1, MERS-CoV a me novel coronaviruses (2019), he mea nui ia o nā maʻi hanu kanaka. ʻO nā hōʻailona pūnaewele e like me ke kuni a me ka luhi, hele pū ʻia me ka ʻū maloʻo a me ka dyspnea, a me nā mea ʻē aʻe, hiki ke ulu koke i loko o ka maʻi pneumonia koʻikoʻi, ka hanu ʻana, a me ka hanu ʻana. he ola-hooweliweli.

ʻO ka 2019-nCOV/COVID-19 IgG/IgM Rapid Test Device he immunoassay chromatographic wikiwiki no ka ʻike qualitative o IgG&IgM antibody o Coronavirus Disease 2019 i loko o ke koko holoʻokoʻa o ke kanaka, serum, a i ʻole ka plasma ma ke ʻano he kōkua i ka hōʻoia ʻana i nā maʻi COVID-19.