【INCAMAKE】

Myoglobin (MYO) ni heme - poroteyine isanzwe iboneka mu mitsi ya skeletale na cardiac ifite uburemere bwa molekile ifite 17.8kDa.Bigizwe na 2 ku ijana bya poroteyine zose z'imitsi kandi ishinzwe gutwara ogisijeni mu ngirangingo z'imitsi. Iyo ingirangingo z'imitsi zangiritse, Myoglobin irekurwa mumaraso byihuse kubera ubunini bwayo. Nyuma y'urupfu rwa tissue ifitanye isano na MI, Myoglobin ni kimwe mu bimenyetso bya mbere byazamutse hejuru y'urwego rusanzwe. Urwego rwa Myoglobin rwiyongera cyane hejuru yumurongo mugihe cyamasaha 2 - 4 post - infarct, igera kumasaha 9 - 12 hanyuma igasubira kumurongo mugihe cyamasaha 24 - 36. Raporo zitari nke zerekana ko gupima Myoglobin nk'imfashanyo yo gusuzuma mu kwemeza ko hatabaho infarction ya myocardial ifite agaciro keza ko guhanura kugera kuri 100% byavuzwe mu bihe bimwe na bimwe nyuma yo gutangira ibimenyetso. Cassette Yihuta ya Myoglobin (Amaraso Yuzuye / Serumu / Plasma) ni ikizamini cyoroshye gikoresha imiti igabanya ubukana - Antibody ya Myoglobin yatwikiriye uduce kandi ifata reagent kugirango tumenye neza Myoglobine mumaraso yose, serumu cyangwa plasma. Urwego ntarengwa rwo kumenya ni 50ng / mL.

【AMABWIRIZA YO GUKORESHA】

Emera ikizamini, icyitegererezo, buffer na / cyangwa igenzura kugera ku bushyuhe bwicyumba (15 - 30 ° C) mbere yo kwipimisha.

1. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho cassette yikizamini mumufuka ufunze hanyuma uyikoreshe mugihe cyisaha imwe.

2. Shira cassette hejuru yisuku kandi iringaniye.

Kuri Serumu cyangwa Plasma urugero:

· Fata igitonyanga gihagaritse hanyuma wohereze ibitonyanga 2 bya serumu cyangwa plasma (hafi 50µL) ahantu hagereranijwe, hanyuma wongereho igitonyanga 1 cya buffer (hafi 40µL), hanyuma utangire igihe. Reba ingero zikurikira.

Kuri Venipuncture Amaraso Yuzuye:

· Fata igitonyanga gihagaritse hanyuma wohereze ibitonyanga 3 byamaraso yose (hafi 75µL) ahantu hatangiwe urugero, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40µL), hanyuma utangire igihe. Reba ingero zikurikira.

Kuri Fingerstick Amaraso Yuzuye:

· Gukoresha umuyoboro wa capillary: Uzuza umuyoboro wa capillary hanyuma wohereze hafi 75µL y'urutoki rw'amaraso yose yerekana amaraso mugace kerekana kaseti yipimishije, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40µL) hanyuma utangire igihe. Reba ingero zikurikira.

· Gukoresha ibitonyanga bimanikwa: Emerera ibitonyanga 3 bimanika urutoki urugero rwamaraso yose (hafi 75µL) kugwa mumwanya wikigereranyo cya cassette, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40 μL) hanyuma utangire igihe. Reba ingero zikurikira.

3. Tegereza umurongo (amabara) ugaragara. Soma ibisubizo muminota 10. Ntugasobanure ibisubizo nyuma yiminota 20.