【GUKORESHA】

Igikoresho CK -

【INCAMAKE】

Myoglobin (MYO), Creatine Kinase MB (CK - MB) na Cardiac Troponin I (cTnI) ni poroteyine zisohoka mu maraso nyuma yo gukomeretsa umutima. Myoglobin ni heme - poroteyine isanzwe iboneka mu mitsi ya skeletale na cardiac ifite uburemere bwa molekile ya 17.8 kDa.Bigizwe na 2 ku ijana bya poroteyine zose z’imitsi kandi ishinzwe gutwara ogisijeni mu ngirabuzimafatizo.1 Iyo ingirangingo z'imitsi zangiritse, Myoglobine irekurwa mu maraso vuba bitewe n'ubunini bwayo buto. Urwego rwa Myoglobin rwiyongera cyane hejuru yumurongo mugihe cyamasaha 2 - 4 post - infarct, kugera kumasaha 9 - 12, hanyuma ugasubira kumurongo mugihe cyamasaha 24 - 36. CK - MB ni enzyme nayo igaragara mumitsi yumutima, ifite uburemere bwa molekile ya 87.0 kDa.4 Creatine Kinase ni molekile ya dimeric igizwe na subunits ebyiri zashyizweho nka "M" na "MM" CK - BB na CKMB. CKMB ni isoenzyme ya Creatine Kinase igira uruhare runini muri metabolisme yimitsi yumutima yumutima.5 Kurekura CK - MB mumaraso nyuma ya MI birashobora kumenyekana mugihe cyamasaha 3 - 8 nyuma yo gutangira ibimenyetso. Ifite impinga mu masaha 9 kugeza 30, ikagaruka kurwego rwibanze mugihe cyamasaha 48 kugeza 72.6 Cardiac Troponin I ni proteine iboneka mumitsi yumutima, ifite uburemere bwa 22.5 kDa.7 Troponine I igizwe nibice bitatu bya subunit bigizwe na Troponin T na Troponine C. Hamwe nibikorwa bya tropomyosine, iyi complexe yimiterere ikora calcium yibintu bya karisiyumu. imitsi.8 Nyuma yo gukomeretsa k'umutima, Troponin I irekurwa mumaraso 4 - amasaha 6 nyuma yububabare butangiye. Uburyo bwo kurekura Troponin I busa na CK - MB, ariko mugihe urwego rwa CK - MB rusubira mubisanzwe nyuma yamasaha 72, Troponin I ikomeza kuzamuka muminsi 6 - 10, bityo igatanga idirishya rirerire ryo kumenya ibikomere byumutima.

Igikoresho CK - Urwego ntarengwa rwo kumenya ni 5 ng / mL CK - MB.

【AMABWIRIZA YO GUKORESHA】

Emera ikizamini, ingero na / cyangwa igenzura kugera ku bushyuhe bwicyumba (1530 ° C) mbere yo kwipimisha.

1. Zana umufuka mubushyuhe bwicyumba mbere yo kuyifungura. Kura igikoresho cyipimisha mumufuka ufunze hanyuma ukoreshe vuba bishoboka. Ibisubizo byiza bizagerwaho niba ikizamini gikozwe ako kanya nyuma yo gufungura umufuka wa file.

2. Shira igikoresho cyo kwipimisha hejuru kandi isukuye.

KuriSerumu cyangwa Plasma ingero:

Fata igitonyanga gihagaritse kandikwimura ibitonyanga 1 bya serumu cyangwa plasma (hafi 25 µL), hanyumaongeramo ibitonyanga 2 bya buffer (hafi 80 µL) hanyuma utangire igihe. Reba ingero hepfo.

KuriVenipuncture Amaraso Yuzuye:

Fata igitonyanga gihagaritse kandikwimura ibitonyanga 2 bya venipuncture maraso yose (hafi 50 µL) kurugero rwiza (S) rwibikoresho byo kwipimisha, hanyumaongeramo ibitonyanga 2 bya buffer (hafi 80 µL) hanyuma utangire igihe. Reba ingero hepfo.

KuriUrutoki Amaraso Yuzuye:

Emera 2 kumanika ibitonyanga byintoki zose zamaraso (hafi 50 µL) kugwa hagati yikigereranyo neza (S) kubikoresho byipimisha, hanyumaongeramo ibitonyanga 2 bya buffer (hafi 80 µL) hanyuma utangire ingengabihe. Reba ingero zikurikira.

3. Tegereza umurongo (amabara) ugaragara.Soma ibisubizo muminota 10.Ntugasobanure ibisubizo nyuma yiminota 20.