【UKUSETYENZISWA OKUHLOSIWEYO】

Isixhobo soVavanyo oluKhawulezayo se-CEA luvavanyo lwe-chromatographic yoqinisekiso olukhawulezayo lomgangatho we-CEA kwigazi elipheleleyo, i-serum okanye i-plasma.Ubuntununtunu kolu vavanyo yi-5 ng/ml. Ukusetyenziswa kobuchwephesha.

【USHWANKATHELO】

I-Carcinoembryonic Antigen (CEA) lithumba-i-antigen enxulumeneyo ephawulwa njenge-oncofetal glycoprotein.CEA ibonakaliswa kwiintlobo ngeentlobo zezifo, ngakumbi imiphunga okanye amathumba esisu (umzekelo, umhlaza wekholoni, umhlaza wesibindi kunye nomhlaza wemiphunga). I-CEA idla ngokubakho kumanqanaba ombungu obonwayo emva kokuzalwa komntwana. amanqanaba aphezulu e-CEA anokuba nexabiso elibalulekileyo kuxilongo lwe-primary carcinomas. Ukongeza kuvavanyo lomgangatho, uvavanyo lweCEA ludlala indima ebalulekileyo ekubekeni iliso kwizigulana ezinomhlaza.

Ubungqina bezonyango bubonisa ukuba amanqanaba e-CEA anokusebenza njengabamakishi abaqikelelwayo kuzo zombini ezi pre- kunye nasemva-nonyango

【IZIKHOKELO ZOKUSETYENZISWA】

Avumela isixhobo sovavanyo, isampulu, isithinteli, kunye/okanye ulawulo ukufikelela kubushushu begumbi (15-30°C) phambi kovavanyo.

1. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokuvula. Susa isixhobo sovavanyo kwisingxobo esivaliweyo kwaye usisebenzise ngokukhawuleza.

2. Beka isixhobo sokuvavanya kwindawo ecocekileyo kunye nenqanaba.

KubaIisampuli zeSerum okanye zePlasma:

Bamba idropha ngokuthe nkqo kwaye udluliseI-1 drop of serum okanye iplasma(malunga ne-25μL) kwindawo yomzekelo, uze wongezeIthontsi enye ye-buffer (malunga ne-40μL), kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

KubaIVenipuncture Iisampulu zeGazi Elipheleleyo:

Bamba idropha ngokuthe nkqo kwaye udluliseAmathontsi e-2 egazi elipheleleyo(malunga ne-50μL) kwindawo yomzekelo, uze wongezeIthontsi enye ye-buffer(malunga ne-40μL), kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

KubaUmnwe Iisampulu zeGazi Elipheleleyo:

Ukusebenzisa ityhubhu ye-capillary: Gcwalisa ityhubhu ye-capillary kunyeugqithiselo malunga50μL yentonga yomnwe isampuli yegazi elipheleleyo kwindawo yomzekelo wesixhobo sovavanyo, uze wongezeIthontsi enye ye-buffer (malunga ne-40μL) kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

3. Lindela u(imi)layini onemibala ukuba ivele.Funda iziphumo ngemizuzu emi-5.Sukutolika iziphumo emva kwemizuzu engama-20.