【ISIFINYEZO】

I-Procalcitonin (PCT) iphrotheni encane ehlanganisa izinsalela ze-amino acid ze-116 ezinesisindo se-molecule cishe i-13 kDa eyachazwa okokuqala ngu-Moullec et al. Ngo-1984. I-PCT ikhiqizwa ngokujwayelekile kuma-C-cells wezindlala zegilo. Ku-1993, izinga eliphakeme le-PCT ezigulini ezinokutheleleka kwesistimu yemvelaphi yebhaktheriya labikwa futhi i-PCT manje ibhekwa njengophawu oluyinhloko lwezinkinga ezihambisana nokuvuvukala kwesistimu kanye ne-sepsis. Inani lokuxilonga le-PCT libalulekile ngenxa yokuhlobana okuseduze phakathi kokuhlushwa kwe-PCT nobukhulu bokuvuvukala. Kwaboniswa ukuthi i-PCT “evuvukalayo” ayikhiqizwa kuma-C-cell. Amaseli omsuka we-neuroendocrine cishe awumthombo we-PCT ngesikhathi sokuvuvukala.

【IZINDLELA ZOKUSEBENZISA】

Vumela ikhasethi lokuhlola, isifanekiso, ibhafa kanye/noma izilawuli ukuthi zilingane nezinga lokushisa legumbi (15-30 ℃) ngaphambi kokuhlolwa.

1. Khipha Ikhasethi Lokuhlola esikhwameni se-foil futhi ulisebenzise ngokushesha ngangokunokwenzeka. Imiphumela emihle kakhulu izotholakala uma ukuhlolwa kwenziwa phakathi nehora elilodwa.

2. Beka Ikhasethi Lokuhlola endaweni ehlanzekile nesezingeni.

Ngezibonelo zeSerum noma zePlasma:

Bamba i-dropper iqonde phezulu bese udlulisela iconsi elingu-1 le-serum noma i-plasma (cishe u-25μL) esibonelweni somthombo (S) Sekhasethi Lokuhlola, bese wengeza iconsi elingu-1 lebhafa (cishe u-40μL), bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

Okwezifanekiso zegazi le-Venipuncture:

Bamba i-dropper ngokuqondile bese udlulisela amaconsi angu-2 egazi eliphelele (cishe u-50μL) esibonelweni somthombo (S) Wekhasethi Lokuhlola, bese wengeza iconsi elingu-1 lebhafa (cishe u-40μL), bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

Okwezifanekiso ze-Fingerstick Whole Blood:

Ukusebenzisa ishubhu le-capillary: Gcwalisa ishubhu le-capillary futhi udlulisele cishe u-50μL wesampula yegazi lomunwe esibonelweni somthombo (S) weKhasethi Lokuhlola, bese wengeza iconsi elingu-1 lebhafa (cishe u-40μL) bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

3. Linda umugqa onemibala uvele. Umphumela kufanele ufundwe ngemizuzu engu-15. Ungahumushi umphumela ngemuva kwemizuzu engama-20.