【ISIFINYEZO】

I-Myoglobin (MYO), i-Creatine Kinase MB (CK-MB) ne-Troponin I yenhliziyo (cTnI) zingamaprotheni akhishelwe egazini ngemva kokulimala kwenhliziyo. I-Myoglobin iyi-heme-protein evamise ukutholakala esicupheni sohlaka lwamathambo nenhliziyo enesisindo samangqamuzana esingu-17.8 kDa. Lapho amangqamuzana emisipha alimala, i-Myoglobin idedelwa egazini ngokushesha ngenxa yobukhulu bayo obuncane. Izinga le-Myoglobin likhuphuka ngokulinganiswa ngaphezu kwesisekelo phakathi kwamahora angu-2-4 kuthunyelwe-i-infarct, lifinyelela phezulu ngamahora angu-9-12, futhi libuyela kusisekelo phakathi kwamahora angu-24-36. I-CK-MB iyi-enzyme ekhona futhi emsipha wenhliziyo, enesisindo samangqamuzana angama-87.0 kDa. I-Creatine Kinase iyi-molecule ye-dimeric eyakhiwe ngamayunithi amabili abizwa ngokuthi “M” kanye “B”, ahlangana akhe ama-isoenzymes amathathu ahlukene, i-CK-MM, CK-BB kanye ne-CK-MB. I-CK-MB iyi-isoenzyme ye-Creatine Kinase ebandakanyeka kakhulu ekusetshenzisweni kwezicubu zemisipha yenhliziyo. Ukukhishwa kwe-CK-MB egazini okulandela i-MI kungatholwa phakathi namahora angu-3-8 ngemva kokuqala kwezimpawu. Iphakama phakathi kwamahora angu-9 kuya kwangu-30, futhi ibuyela emazingeni okuqala phakathi kwamahora angu-48 kuya kwangu-72. I-Cardiac Troponin I iphrotheni etholakala kumisipha yenhliziyo, enesisindo samangqamuzana angama-22.5 kDa. I-Troponin I iyingxenye ye-subunit complex ehlanganisa i-Troponin T ne-Troponin C. Kanye ne-tropomyosin, le nkimbinkimbi yesakhiwo yakha ingxenye eyinhloko elawula umsebenzi we-ATPase ozwela nge-calcium we-actomyosin kumisipha yohlaka lwamathambo nenhliziyo. Ngemuva kokulimala kwenhliziyo kwenzeka, i-Troponin I ikhishwa egazini 4-6 amahora ngemva kokuqala kobuhlungu. Iphethini yokukhululwa ye-Troponin I ifana ne-CK-MB, kodwa ngenkathi amazinga e-CK-MB ebuyela kokujwayelekile ngemva kwamahora angu-72, i-Troponin I ihlala iphakeme izinsuku ezingu-6-10, ngaleyo ndlela inikeze iwindi elide lokutholwa kokulimala kwenhliziyo.

I-Myoglobin/CK-MB/Troponin I Combo Rapid Test Cassette (Whole Blood/Serum/Plasma) iwukuhlola okulula okusebenzisa inhlanganisela yezinhlayiya ezimbozwe i-antibody kanye nokuthwebula ama-reagents ukuze kutholwe kahle i-Myoglobin, CK-MB kanye ne-cardiac Troponin I (cTnIrum) noma i-plasma, i-plasma yonke. Izinga eliphansi lokutholwa ngu-50ng/mL Myoglobin, 5ng/mL CK-MB kanye no-0.5ng/mL Troponin I.

 【IZINDLELA ZOKUSEBENZISA】

Vumela ikhasethi lokuhlola, isifanekiso, ibhafa kanye/noma izilawuli ukuthi zifinyelele izinga lokushisa legumbi (15-30°C) ngaphambi kokuhlolwa.

1. Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula. Susa ikhasethi lokuhlola esikhwameni esivaliwe futhi ulisebenzise ngokushesha ngangokunokwenzeka.

2. Beka ikhasethi endaweni ehlanzekile nesezingeni.

Ngeserum noma isampula sePlasma:

Bamba i-dropper ngokuqondile bese udlulisela amaconsi angu-2 e-serum noma i-plasma (cishe u-50µL) kusifanekiso kahle, bese wengeza iconsi elingu-1 lebhafa (cishe u-40µL), bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

Okwesibonelo se-Venipuncture Igazi Eliphelele:

Bamba i-dropper ngokuqondile bese udlulisela amaconsi angu-2 egazi eliphelele (cishe u-50µL) kusifanekiso kahle, bese wengeza iconsi elingu-1 lebhafa (cishe u-40µL), bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

Ngesifanekiso se-Fingerstick Whole Blood:

· Ukusebenzisa ishubhu le-capillary: Gcwalisa ishubhu le-capillary futhi udlulisele cishe u-50µL wesampula segazi lomunwe esibonelweni somthombo wekhasethi lokuhlola, bese wengeza iconsi elingu-1 lebhafa (cishe u-40µL) bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

· Ukusebenzisa amaconsi alengayo: Vumela amaconsi alengayo angu-2 omunwe wesampula yegazi lonke (cishe u-50 µL) ukuze awele endaweni yesifanekiso sekhasethi lokuhlola, bese wengeza iconsi elingu-1 lebhafa (cishe u-40µL) bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

3. Linda kuze kuvele i(ama)layini anombala. Funda umphumela ngemizuzu eyi-10. Ungahumushi umphumela ngemuva kwemizuzu engama-20.