Ibyiyumvo byihariye kandi byihariye: 2019 - nCOV / COVID - 19 IgG / IgM Igikoresho cyihuta cyikigereranyo cyagereranijwe nigeragezwa rikomeye ry’ubucuruzi RT - PCR ikoresheje ingero z’amavuriro.Ibisubizo byerekana ko 2019 - nCoV / COVID - 19 IgG / IgM Igikoresho cyihuta cyihuta kandi gifite umwihariko.

Kwipimisha IgG:

Ibyiyumvo bifitanye isano: 233/268 = 86,94% (95% CI *: 82.35% - 90.49%)

Umwihariko ugereranije: 287/289 = 99.31% (95% CI *: 97.52% - 99.92%)

Ukuri: 520/557 = 93.36% (95% CI *: 90,96% - 95.16%)

Intera y'icyizere

Kugerageza IgM

Ibyiyumvo bifitanye isano: 223/268 = 83.21% (95% CI *: 78.19% - 87.48%)

Umwihariko ugereranije: 282/289 = 97.58% (95% CI *: 95.07% - 99.02%)

Ukuri: 505/557 = 90.66% (95% CI *: 87,94% - 92,95%)

Intera y'icyizere

2019 - nCOV / COVID - 19 IgG / IgM Igikoresho cyihuta cyihuta ni immunoassay yihuse ya chromatografique kugirango hamenyekane ubuziranenge bwa IgG & IgM antibody ya Coronavirus Disease 2019 mumaraso yose yumuntu, serumu, cyangwa plasma nkubufasha mugupima indwara ya COVID - 19.

GUKORESHA BIKORESHEJWE: 2019 - nCOV / COVID - 19 IgG / IgM Igikoresho cyihuta cyihuta ni immunoassay yihuse ya chromatografique yo kumenya neza antibody IgG & IgM antibody ya Coronavirus Disease 2019 mumaraso yabantu yose, serumu, cyangwa plasma nkubufasha mugupima indwara ya COVID - 19.

INCAMAKE: COVID - 19 ni indwara yandurira mu myanya y'ubuhumekero. Abantu muri rusange birashoboka. Kugeza ubu, abarwayi banduye igitabo cyitwa coronavirus ni isoko nyamukuru yo kwandura; abantu banduye badafite ibimenyetso na bo barashobora kuba isoko yanduye. Ukurikije iperereza ryakozwe muri iki gihe, igihe cyo gukuramo ni iminsi 1 kugeza 14, ahanini iminsi 3 kugeza 7. Ibyigaragaza nyamukuru birimo umuriro, umunaniro hamwe no gukorora byumye. Umubyimba wizuru, izuru ritemba, kubabara mu muhogo, myalgia na diarrhea usanga mubihe bike.

AMABWIRIZA YO GUKORESHA: Emerera igikoresho, ikigereranyo, buffer, na / cyangwa igenzura kugera ku bushyuhe bwicyumba (15 - 30 ° C) mbere yo kwipimisha. 1. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho igikoresho cyipimisha mumufuka ufunze hanyuma ukoreshe vuba bishoboka. 2. Shira igikoresho cyo kwipimisha hejuru kandi isukuye. ? Kuri Serumu cyangwa Plasma Ibigereranyo: Ukoresheje imiyoboro ya 10μL yatanzwe, shushanya urugero kugeza kuri Fill Line, hanyuma wohereze 10μL serumu / plasma kurugero rwiza rwibikoresho bipimisha, hanyuma wongereho ibitonyanga 2 bya buffer hanyuma utangire igihe. ? Kumaraso Yuzuye (Venipuncture / Fingerstick) Ingero: Ukoresheje umuyoboro wa 10μL watanzwe, hanyuma wohereze igitonyanga 1 cyamaraso yose (hafi 20μL) kurugero rwiza rwibikoresho bipimisha, hanyuma ongeramo ibitonyanga 2 bya buffer hanyuma utangire igihe. Icyitonderwa: Ibigereranyo birashobora kandi gukoreshwa ukoresheje micropipette. 3. Tegereza umurongo (amabara) ugaragara. Soma ibisubizo muminota 10. Ntugasobanure ibisubizo nyuma yiminota 15.

INGINGO: Iki gikoresho gikoresha immunochromatografiya. Ikarita yikizamini ikubiyemo: 1) zahabu ya colloidal - yanditseho recombinant novel coronavirus antigen hamwe na antibody igenzura ubuziranenge bwa zahabu; Umurongo M uhagarikwa hamwe na anti-monoclonal anti - antibody ya muntu IgM yo kumenya antibody ya coronavirus IgM antibody; umurongo wa IgG wimuwe hamwe na reagent yo kumenya agashya coronavirus IgG antibody; n'umurongo C uhagarikwa hamwe na antibody yo kugenzura ubuziranenge. Iyo umubare ukwiye w'icyitegererezo wongeyeho ku mwobo w'ikarita y'ikizamini, icyitegererezo kizakomeza imbere yikarita yikizamini munsi ya capillary.Niba icyitegererezo kirimo antibody ya IgM, antibody izahuza na zahabu ya colloidal - yanditseho antigen coronavirus antigen. Ubudahangarwa bw'umubiri buzafatwa na anti - antibody ya muntu IgM yandujwe kuri membrane kugirango ibe umurongo wumutuku - umutuku M, werekana ko igitabo cyitwa coronavirus IgM antibody ari cyiza. Niba icyitegererezo kirimo antibody ya IgG, antibody izahuza na zahabu ya colloidal - yanditseho antigen coronavirus antigen, kandi immunite izafatwa na reagent yimibilisite kuri membrane kugirango ibe umurongo wumutuku - umurongo wa IgG utukura, byerekana ko antibody ya coronavirus IgG ari nziza. Niba ikizamini IgG na IgM kidafite ibara, hagaragajwe ibisubizo bibi. Ikarita yikizamini nayo irimo umurongo ugenzura ubuziranenge C.Umurongo wo kugenzura ubuziranenge bwa fuchsia C ugomba kugaragara utitaye ko umurongo wikizamini ugaragara. Niba umurongo ugenzura ubuziranenge C utagaragara, ibisubizo byikizamini ntabwo byemewe, kandi icyitegererezo gikeneye kongera kugeragezwa hamwe nindi karita yikizamini.