【GUKORESHA】

Ikizamini cyihuta cya Ethyl Glucuronide (ETG) ni immunoassay yihuta ya chromatografique yo kumenya Ethyl Glucuronide mu nkari zabantu. Ethyl Glucuronide yagaragaye n'ikizamini ikubiyemo, ariko ntabwo igarukira gusa kuri metabolite ya Ethanol.

Iyi suzuma itanga gusa ibisubizo byibanze byisesengura. Uburyo bwihariye bwo guhinduranya imiti bugomba gukoreshwa kugirango tubone ibisubizo byemejwe byisesenguye. Gazi ya chromatografiya / mass spectrometrie (GC / MS) nuburyo bwatoranijwe bwo kwemeza. Kwita ku mavuriro no guca imanza z'umwuga bigomba gukoreshwa ku biyobyabwenge byose byakorewe ibizamini, cyane cyane iyo hakoreshejwe ibisubizo byiza byambere.

【INCAMAKE】

Ethyl Glucuronide (ETG) ni metabolite ya alcool ya Ethyl ikorwa mu mubiri byglucuronidation nyuma yo guhura na Ethanol, nko kunywa ibinyobwa bisindisha.Bikoreshwa nka biomarker mu gupima ikoreshwa rya Ethanol no kugenzura ko kwirinda inzoga mu bihe aho kunywa ibiyobyabwenge biboneka, nko mu gisirikare, muri gahunda z’ubugenzuzi bw’indege, abashinzwe umutekano, abashinzwe umutekano mu ndege, abashinzwe umutekano mu ndege, abashinzwe umutekano mu ndege, abashinzwe umutekano mu ndege, abashinzwe umutekano mu ndege, abashinzwe umutekano mu ndege, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano, abashinzwe umutekano mu ndege, bongeraho amavuriro, cyangwa mugukiza abarwayi ba alcool.ETG irashobora gupimwa mu nkari nuburyo bwa laboratoire gakondo (GC / MS cyangwa LC / MS) kugeza amasaha agera kuri 80 nyuma ya Ethanol imaze guterwa (biterwa cyane nubunini bwibinyobwa bisindisha kandi bitari - inzoga). ETG ni ikimenyetso cyerekana neza ko inzoga zirenze urugero kuruta gupima ahari Ethanol ubwayo.

Ikizamini cya Ethyl Glucuronide (Urine) ni ikizamini cyihuta cyo gusuzuma inkari gishobora gukorwa udakoresheje igikoresho. Ikizamini gikoresha antibody ya monoclonal kugirango ihitemo kugirango igaragaze urugero rwinshi rwa Ethyl glucuronide mu nkari zabantu. Ikizamini cya Ethyl Glucuronide Yihuta (Urine) gitanga umusaruro mwiza mugihe Ethyl Glucuronide mu nkari irenze 500 ng / mL.

【AMABWIRIZA YO GUKORESHA】

Emera ikizamini, urugero rwinkari, na / cyangwa kugenzura kugera kubushyuhe bwicyumba (15 - 30ºC) mbere yo kwipimisha.

1.Kuzana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho igikoresho cyikizamini mumufuka ufunze hanyuma ukoreshe mugihe cyisaha imwe.

2. Shira igikoresho cyikizamini hejuru yisuku kandi iringaniye. Fata igitonyanga gihagaritse hanyuma wohereze ibitonyanga 3 byuzuye byinkari (hafi 120ul) kurugero rwiza (S) rwibikoresho bipimisha, hanyuma utangire igihe. Irinde gufata impemu zo mu kirere mu buryo bwiza (S). Reba ikigereranyo gikurikira.

3.Tegereza umurongo (amabara) ugaragara. Soma ibisubizo muminota 5. Ntugasobanure ibisubizo nyuma yiminota 10.

Icyerekezo cyibizamini

4. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho ikizamini cyibizamini mumufuka ufunze hanyuma ukoreshe vuba bishoboka.

5. Hamwe n'imyambi yerekana urugero rw'inkari, shira umurongo wikizamini uhagaritse mu nkari byibuze amasegonda 10 kugeza kuri 15.Shira umurongo byibuze kurwego rwumurongo wa wavy, ariko nturengere umurongo ntarengwa (MAX) kumurongo wikizamini. [Reba ishusho (2).]

6. Shira umurongo wikizamini hejuru yubutaka butari -

7. Tangira ingengabihe hanyuma utegereze umurongo (amabara) ugaragara.

8. Ibisubizo bigomba gusomwa muminota 5. Ibisubizo birashobora kuba bihamye kugeza isaha 1 nyuma yo gutangira ikizamini.

Icyerekezo cy'ikarita y'Ikizamini

1. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho ikarita yikizamini mu mufuka ufunze kandi uyikoreshe vuba bishoboka.

2. Kuraho ingofero.

3.Nukoresheje umwambi werekeza ku nkari, shira ikarita yikizamini uhagaritse mu nkari byibuze amasegonda 10 kugeza kuri 15.Shira umurongo byibuze kurwego rwumurongo wa wavy, ariko ntabwo hejuru yumwambi kurikarita yikizamini. [Reba ishusho (1).]

4.Simbuza ingofero hanyuma ushire ikarita yikizamini hejuru yubutaka butagaragara -

5.Tangira ingengabihe hanyuma utegereze umurongo (amabara) ugaragara.

6.Ibisubizo bigomba gusomwa muminota 5. Ibisubizo birashobora kuba bihamye kugeza isaha 1 nyuma yo gutangira ikizamini.