Ukuzwela nokucacisa: Idivayisi ka-2019-nCOV/COVID-19 IgG/IgM Rapid Test iqhathaniswe nokuhlola okuhamba phambili kwe-RT-PCR yezentengiselwano kusetshenziswa amasampula omtholampilo. Imiphumela ibonisa ukuthi i-2019-nCoV/COVID-19 IgG/IgM Rapid Test Device inokuzwela okuphezulu nokucacile.

Ngokuhlolwa kwe-IgG:

Ukuzwela Okuhlobene:233/268=86.94%(95%CI*:82.35%-90.49%)

Ukucaciswa okuhlobene:287/289=99.31% (95%CI*:97.52%-99.92%)

Ukunemba:520/557=93.36% (95%CI*: 90.96%-95.16%)

*Isikhawu Sokuzethemba

Okokuhlolwa kwe-IgM

Ukuzwela Okuhlobene:223/268=83.21% (95%CI*:78.19%-87.48%)

Ukucaciswa okuhlobene:282/289=97.58% (95%CI*: 95.07%-99.02%)

Ukunemba: 505/557=90.66% (95%CI*: 87.94%-92.95%)

*Isikhawu Sokuzethemba

ISIMISO: Le kit isebenzisa i-immunochromatography.Ikhadi lokuhlola liqukethe:1) igolide le-colloidal-elilebulwe kabusha ukuthi i-coronavirus antigen kanye nezimpawu zegolide zokulawula i-antibody;2) imigqa emibili yokuthola (imigqa ye-IgG ne-IgM) kanye nomugqa owodwa wokulawula ikhwalithi (umugqa C) we-nitrocellulose membrane. inoveli coronavirus I-antibody ye-IgM; ulayini we-IgG awunyakazi nge-reagent yokuthola i-antibody ye-IgG yenoveli; futhi ulayini u-C awunyakazi ne-antibody elawula ikhwalithi. Uma inani elifanele lesampula lokuhlola lengezwa embotsheni yesampula yekhadi lokuhlola, isampula izoqhubekela phambili eduze nekhadi lokuhlola ngaphansi kwesenzo se-capillary.Uma isampula iqukethe i-antibody ye-IgM, i-antibody izobophezela ku-colloidal gold-ebhalwe inoveli coronavirus antigen. I-immune complex complex izothunjwa i-anti-human IgM antibody engakwazi ukuhamba kulwelwesi ukwenza ulayini we-IgM onsomi-obomvu, okubonisa ukuthi i-antibody ye-coronavirus ye-IgM ine-positive. Uma isampula iqukethe i-antibody ye-IgG, i-antibody izobophezela kugolide we-colloidal-okubhalwe ukuthi inoveli coronavirus antigen, futhi ukuzivikela komzimba kuzobanjwa ulwelwesi lwe-IgM ukuze lufakwe kabusha ulwelwesi lwe-IgG - umugqa, obonisa ukuthi inoveli coronavirus I-antibody ye-IgG i-positive.Uma imigqa yokuhlola ye-IgG ne-IgM ingenambala, kuvela umphumela ongemuhle.Ikhadi lokuhlola liqukethe nomugqa wokulawula ikhwalithi C.Umugqa wokulawula ikhwalithi we-fuchsia kufanele uvele kungakhathaliseki ukuthi umugqa wokuhlola uyavela yini.Umugqa wokulawula ikhwalithi uyibhande lombala lokulawula ikhwalithi i-antibody immune complex. Uma ulayini wokulawula ikhwalithi C ungaveli, umphumela wokuhlolwa awuvumelekile, futhi isampula idinga ukuhlolwa futhi ngelinye ikhadi lokuhlola.

Nakhu okudingeka ukwenze ukuze wenze ukuhlolwa:

1.Hlanza umunwe nge-alcohol swab.

2.Hlaba umunwe ofanayo ngomunwe ukuze uthole isampula yegazi.

3.Ukukhulula ikhava yenaliti jikisa imicibisholo elandela iwashi lesembozo.

4.Sebenzisa idropha ukuze udonse igazi ozolifaka ekhasethini lokuhlola.

5.Hlola amamaki ekhasethi ngemva kwemizuzu eyi-10 ukuze ubone umphumela.

ICoronavirus (CoV) iyingxenye yohlobo lwe-Nestovirus, i-Coronaviridae, futhi ihlukaniswe yaba izigaba ezintathu: α, β, kanye no-γ.Uhlobo α kanye no-β luyingozi kuphela ezilwaneni ezincelisayo.Uhlobo luka-γ lubangela izifo zezinyoni kakhulu. Kukhona nobufakazi bokuthi ingadluliselwa ngomzila we-fecal-oral.

Kuze kube manje, kunezinhlobo ezingu-7 ze-human coronavirus (HCoV) ezibangela izifo zokuphefumula komuntu: i-HCoV-229E,HCoV-OC43,SARS-CoV,HCoV-NL63,HCoV-HKU1,MERS-CoV kanye ne-novel coronaviruses (2019), iyigciwane elibalulekile lezifo zokuphefumula komuntu.Phakathi kwayo, kwatholakala i-coronavirus engu-201 emtholampilo. izimpawu zesistimu ezifana nemfiva nokukhathala, okuhambisana nokukhwehlela okomile kanye ne-dyspnea, njll., okungase kukhule ngokushesha kube inyumoniya enzima, ukwehluleka ukuphefumula, nokuphefumula kanzima.I-Distress syndrome, ukushaqeka kwe-septic, ukwehluleka kwezitho eziningi, i-asidi enzima-izinkinga ze-base metabolism, njll. zilingana nempilo-zisongela.

I-2019-nCOV/COVID-19 IgG/IgM Rapid Test Device iyi-chromatographic immunoassay esheshayo yokutholwa kwekhwalithi ye-IgG&IgM antibody ye-Coronavirus Disease 2019 egazini lomuntu lonke, i-serum, noma i-plasma njengosizo ekuxilongweni kwezifo ze-COVID-19.