【Ezubere iji】

Ngwa TOXO IgG/IgM Ngwa ngwa ngwa (Ọbara dum/Plasma/Serum) bụ n'akụkụ eruba chromatographic immunoassay maka nchọpụta otu oge na ọdịiche nke IgM mgbochi-Toxoplasma Gondii(T.gondii) na IgG mgbochi - T.gondii na ọbara mmadụ, a ga-eji nke a dịka nyocha ọbara zuru oke. na nchoputa nke ọrịa na T.gondii. A ga-eji usoro nnwale ọzọ na nchoputa nyocha TOXO IgG/IgM na-eme ngwa ngwa (Ọbara dum/Plasma/Serum) ga-egosi ụdị nlegharị anya ọ bụla.

【Nchịkọta】

T.gondii bụ parasaiti protozoan intracellular nke nwere nkesa zuru ụwa ọnụ1.2. Ihe omuma nke serological na-egosi na ihe dika 30% nke otutu obodo mepere emepe bu oria na-adighi ala. A na-eji ule serologic dị iche iche maka ọgwụ nje na T.gondii dị ka ihe enyemaka na nchọpụta nke nnukwu ọrịa na iji nyochaa n'oge gara aga na organism. Nlele ndị a bụ Sabin-Feldman dye test, direct agglutination, indirect hemagglutination, latex agglutination, indirect immunofluorescence na ELcentISA47 na-aga n'ihu. TOXO IgG/lgG Ngwa nnwale ngwa ngwa(Ọbara dum/Plasma/Serum) ka ewebatara n'ụlọọgwụ maka serodiagnosis nke ọrịa T.gondi.

【Ntuziaka maka ojiji】

Kwe ka nnwale ahụ, ihe nlere, ihe nchekwa na/ma ọ bụ njikwa rute ọnụ ụlọ (15 -30°C) tupu nnwale.

1. Weta obere akpa ahụ na okpomọkụ tupu imeghe ya. Wepu ngwaọrụ nnwale ahụ na obere akpa mkpuchi ma jiri ya ozugbo enwere ike.

2. Debe ngwaọrụ ahụ n'elu ala dị ọcha ma dị larịị.

MakaSerum ma ọ bụ Plasma:

Jide dobe ahụ kwụ ọtọ, see ihe nlele ahụ ruo ahịrị nju, nanyefee 10 μl serum ma ọ bụ plasma gaa na mpaghara ihe atụ, mgbe ahụtinye 2 tụlee nke ihe nchekwa (ihe dị ka 80μl),ma malite ngụ oge.Lee ihe atụ n'okpuru.

MakaỤdị Ọbara Nile Venipuncture:

Jide dobe ahụ kwụ ọtọ nanyefee 2 tụlee(ihe dịka 20μl) nke ọbara dum gaa na mpaghara ihe atụ, mgbe ahụtinye 2 tụlee nke ihe nchekwa (ihe dị ka 80μl), ma malite ngụ oge. Lee ihe atụ n'okpuru.

MakaNlere ọbara dum mkpịsị aka:

Iji jiri tube capillary: Jujuo tube capillary wee bufee 2 tụlee (ihe dị ka 20μl) nke mkpịsị mkpịsị aka ọbara n'akụkụ ihe nlele ngwaọrụ, wee tinye 2 tụlee nke ihe nchekwa (ihe dị ka 80μl) wee malite ngụ oge.

3. Chere ka ahịrị(s) nwere agba pụta.Gụọ nsonaazụ na nkeji iri na ise.Atụgharịla nsonaazụ ya mgbe nkeji iri abụọ gachara.