【GUKORESHA】

TOXO IgG / IgM Igikoresho Cyihuta Cyipimisha (Amaraso Yuzuye / Plasma / Serum) ni immunoassay ya chromatografique yo gutembera mugihe kimwe cyo gutahura no gutandukanya icyarimwe IgM anti - Toxoplasma Gondii (T.gondii) na IgG anti - T.gondii muri serumu yabantu, plasma cyangwa amaraso yose.Iki gikoresho kigenewe gukoreshwa nkigipimo cyo gusuzuma no kwanduza T. Ikigereranyo icyo ari cyo cyose gifatika hamwe na TOXO IgG / IgM Igikoresho cyihuta cyipimisha (Amaraso Yuzuye / Plasma / Serum) kigomba kwemezwa hakoreshejwe ubundi buryo bwo kwipimisha hamwe nubuvuzi.

【INCAMAKE】

T.gondii ni parasite yo mu bwoko bwa protozoan parasite hamwe nogukwirakwiza kwisi yose.2. Amakuru ya serologiya yerekana ko hafi 30% byabaturage b’ibihugu byinshi byateye imbere banduye ibinyabuzima igihe kirekire. Ibizamini bitandukanye bya serologique kuri antibodies kuri T.gondii byakoreshejwe nkubufasha mu gusuzuma indwara zanduye ndetse no gusuzuma ibyabanje guhura n’ibinyabuzima.Ibi bizamini ni ikizamini cy’irangi rya Sabin - Feldman, aglutination itaziguye, hemagglutination itaziguye, latex agglutination, immunofluorescence itaziguye na ELISA4 - Igikoresho (Amaraso Yuzuye / Plasma / Serum) cyinjijwe mu ivuriro rya serodiagnose yanduye T.gondi.

【AMABWIRIZA YO GUKORESHA】

Emera ikizamini, icyitegererezo, buffer na / cyangwa igenzura kugera ku bushyuhe bwicyumba (15 - 30 ° C) mbere yo kwipimisha.

1. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho igikoresho cyipimisha mumufuka ufunze hanyuma ukoreshe vuba bishoboka.

2. Shira Igikoresho hejuru yisuku kandi iringaniye.

KuriSerumu cyangwa Plasma urugero:

Fata igitonyanga gihagaritse, shushanya ingero kugeza Kuzuza Umurongo, nakwimura 10μl serumu cyangwa plasma Kuri icyitegererezo, hanyumaongeramo ibitonyanga 2 bya buffer (hafi 80μl), hanyuma utangire igihe. Reba ingero hepfo.

KuriVenipuncture Amaraso Yuzuye:

Fata igitonyanga gihagaritse kandikwimura ibitonyanga 2（hafi 20μl) y'amaraso yose Kuri icyitegererezo, hanyumaongeramo ibitonyanga 2 bya buffer (hafi 80μl), hanyuma utangire igihe. Reba ingero hepfo.

KuriUrutoki Amaraso Yuzuye:

Kugira ngo ukoreshe umuyoboro wa capillary: Uzuza umuyoboro wa capillary hanyuma wohereze ibitonyanga 2 (hafi 20μl) by'urutoki rw'amaraso yose yerekana urugero rw'ibikoresho by'ibizamini, hanyuma wongereho ibitonyanga 2 bya buffer (hafi 80μl) hanyuma utangire igihe.

3. Tegereza umurongo wamabara ugaragara.Soma ibisubizo muminota 15.Ntugasobanure ibisubizo nyuma yiminota 20.