Kukhudzika ndi Kudziwikiratu: Chida cha 2019-nCOV/COVID-19 IgG/IgM Rapid Test Chipangizo chayerekezedwa ndi makina oyesera a RT-PCR otsogola pogwiritsa ntchito zitsanzo zachipatala. Zotsatira zikusonyeza kuti 2019-nCoV/COVID-19 IgG/IgM Rapid Test Chipangizo chili ndi mphamvu zokhuza kwambiri.

Kwa mayeso a IgG:

Kukhudzidwa Kwachibale:233/268=86.94%(95%CI*:82.35%-90.49%)

Mwachibale:287/289=99.31% (95%CI*:97.52%-99.92%)

Kulondola:520/557=93.36% (95%CI*: 90.96%-95.16%)

* Nthawi Yachikhulupiriro

Kwa mayeso a IgM

Kukhudzidwa Kwachibale:223/268=83.21% (95%CI*:78.19%-87.48%)

Mwachibale:282/289=97.58% (95%CI*: 95.07%-99.02%)

Kulondola: 505/557=90.66% (95%CI*: 87.94%-92.95%)

* Nthawi Yachikhulupiriro

Chida cha 2019-nCOV/COVID-19 IgG/IgM Rapid Test Device ndi njira yofulumira kwambiri yodziwira matenda a IgG&IgM a Coronavirus Disease 2019 m'magazi amunthu, seramu, kapena madzi a m'magazi monga chothandizira kuzindikira matenda a COVID-19.

KUGWIRITSA NTCHITO CHOFUNIKA: The 2019-nCOV/COVID-19 IgG/IgM Rapid Test Device ndi njira yofulumira kwambiri yodziwira matenda a IgG&IgM ya matenda a Coronavirus 2019 m'magazi athunthu amunthu, seramu, kapena plasma ngati chothandizira pa matenda a COVID19.

DZIWANI IZI: COVID - 19 ndi matenda opatsirana pachimake. Nthawi zambiri anthu amavutika. Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye gwero lalikulu la matenda; anthu omwe ali ndi matenda asymptomatic amathanso kukhala gwero lopatsirana. Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yoyamwitsa ndi masiku 1 mpaka 14, makamaka masiku 3 mpaka 7. The mawonetseredwe aakulu monga malungo, kutopa ndi youma chifuwa. Kutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka nthawi zingapo.

ZOYENERA KUGWIRITSA NTCHITO: Lolani chipangizo choyesera, chitsanzo, bafa, ndi/kapena zowongolera kuti zifikire kutentha (15-30°C) musanayesedwe. 1. Bweretsani thumbalo kuti lizizizira bwino musanatsegule. Chotsani chipangizo choyesera m'thumba lomata ndikuchigwiritsa ntchito posachedwa. 2. Ikani chipangizo choyesera pamalo oyera komanso osakanikirana. ? Pa Zitsanzo za Seramu kapena Plasma: Pogwiritsa ntchito pipette yotayika ya 10μL, jambulani chitsanzocho ku Fill Line, ndikusamutsa 10μL seramu/plasma pachitsime cha chipangizo choyesera, kenako onjezani madontho awiri a buffer ndikuyamba chowerengera. ? Kwa Zitsanzo za Magazi Athunthu (Venipuncture/Fingerstick): Pogwiritsa ntchito pipette yotayidwa ya 10μL yoperekedwa, ndi kusamutsa dontho limodzi la magazi athunthu (pafupifupi 20μL) ku chitsime chachitsanzo cha chipangizo choyesera, kenaka yikani madontho awiri a bafa ndi kuyambitsa chowerengera. Zindikirani: Zitsanzo zitha kugwiritsidwanso ntchito pogwiritsa ntchito micropipette. 3. Dikirani kuti mizere yamitundu iwonekere. Werengani zotsatira pakadutsa mphindi 10. Osamasulira zotsatira pakadutsa mphindi 15.

MFUNDO: Chidachi chimagwiritsa ntchito immunochromatography.Khadi loyesera lili ndi:1) colloidal gold-yolembedwa recombinant novel coronavirus antigen ndi zolembera zagolide za antibody;2) mizere iwiri yodziwira (mizere ya IgG ndi IgM) ndi mzere umodzi Wowongolera (C mzere) wa nembanemba ya nitrocellulose. Mzere wa M umakhala wosasunthika ndi anti-munthu IgM antibody kuti azindikire kachilombo ka corona IgM antibody; mzere wa IgG umakhala wosasunthika ndi reagent yozindikira kachilombo ka HIV IgG antibody; ndipo C line imakhala yosasunthika ndi antibody control control. Ngati kuchuluka koyenera kwa mayesowo kuwonjezeredwa pa dzenje lachitsanzo la khadi loyeserera, chitsanzocho chimapita patsogolo pakhadi yoyeserera pansi pa ma capillary. Chitetezo cha mthupi chidzagwidwa ndi anti-anthu IgM antibody osasunthika pa nembanemba kuti apange chibakuwa-mzere wofiira wa M, kuwonetsa kuti buku la coronavirus IgM antibody lili ndi chiyembekezo. Ngati chitsanzocho chili ndi anti-IgG, antibody imamanga ku colloidal golide-yolemba buku la coronavirus antigen, ndipo chitetezo chamthupi chidzagwidwa ndi reagent osasunthika pa nembanemba kuti apange chibakuwa-mzere wofiyira wa IgG, kusonyeza kuti buku la coronavirus IgG antibody lili ndi chiyembekezo. Ngati mizere ya IgG ndi IgM yoyesedwa ilibe mitundu, zotsatira zoipa zikuwonetsedwa.Khadi loyesera limakhalanso ndi mzere wowongolera khalidwe C. Fuchsia quality control line C iyenera kuwonekera mosasamala kanthu kuti mzere woyesera ukuwonekera.Mzere wowongolera khalidwe ndi mtundu wa mtundu wa khalidwe lolamulira antibody immune complex. Ngati mzere wowongolera khalidwe C sukuwonekera, zotsatira zake ndizosavomerezeka, ndipo chitsanzocho chiyenera kuyesedwanso ndi khadi lina loyesa.