【ZOFUNIKA KUGWIRITSA NTCHITO】

Ethyl Glucuronide (ETG) Rapid Test (Mkodzo) ndi chromatographic immunoassay yofulumira pozindikira Ethyl Glucuronide mumkodzo wamunthu. The Ethyl Glucuronide wapezeka ndi mayeso akuphatikizapo, koma si zokhazo, metabolites Ethanol.

Kuyesa uku kumangopereka zotsatira zoyambirira zowunikira. Njira ina yodziwika bwino yamankhwala iyenera kugwiritsidwa ntchito kuti mupeze zotsatira zotsimikizika. Gas chromatography/mass spectrometry (GC/MS) ndiyo njira yotsimikizirira yokondedwa. Kulingalira zachipatala ndi kuweruza kwa akatswiri kuyenera kugwiritsidwa ntchito pazotsatira zilizonse zoyeserera zakugwiritsa ntchito molakwa mankhwala, makamaka ngati zotsatira zoyambilira zikugwiritsidwa ntchito.

【CHIDULE】

Ethyl Glucuronide (ETG) ndi metabolite wa mowa wa ethyl womwe umapangidwa m'thupi ndi glucuronidation pambuyo pakumwa mowa mowa, monga kumwa zakumwa zoledzeretsa. Imagwiritsidwa ntchito ngati chizindikiro cha biomarker poyesa kugwiritsa ntchito Mowa komanso kuyang'anira kusamwa mowa pakakhala zoletsedwa kumwa mowa (monga kuyang'anira mapologalamu ankhondo, monga akatswiri ankhondo (monga kuyang'anira ntchito zankhondo) akatswiri, maloya, oyendetsa ndege akuchira ku zizoloŵezi zoipa), m'masukulu, m'zipatala zopangira inliver, kapena pochira odwala zidakwa.ETG ikhoza kuyesedwa mumkodzo pogwiritsa ntchito njira zachikhalidwe za labotale (GC/MS kapena LC/MS) mpaka pafupifupi maola 80 mutatha kumwa mowa (zodalira kwambiri kuchuluka kwa mowa ndi zakumwa zoledzeretsa). ETG ndi chizindikiro cholondola kwambiri chakumwa mowa kwambiri kuposa kuyeza kukhalapo kwa ethanol komweko.

Ethyl Glucuronide Rapid Test (Mkodzo) ndi kuyesa kofulumira kwa mkodzo komwe kumatha kuchitika popanda kugwiritsa ntchito chida. Mayesowa amagwiritsa ntchito anti-monoclonal antibody kuti azindikire kuchuluka kwa ethyl glucuronide mumkodzo wamunthu. Ethyl Glucuronide Rapid Test Strip (Mkodzo) umapereka zotsatira zabwino pamene Ethyl Glucuronide mu mkodzo umaposa 500 ng/mL.

【MALANGIZO OGWIRITSA NTCHITO】

Lolani kuyesa, chitsanzo cha mkodzo, ndi/kapena zowongolera kuti zifike kutentha (15-30ºC) musanayesedwe.

1.Bweretsani thumba mu kutentha kwa chipinda musanatsegule. Chotsani kuyesa Chipangizo muthumba losindikizidwa ndikuchigwiritsa ntchito mkati mwa ola limodzi.

2.Ikani kuyesa Chipangizo pamalo oyera komanso okwera. Gwirani dontho molunjika ndi kusamutsa madontho atatu a mkodzo (pafupifupi 120ul) ku chitsanzo chabwino (S) cha chipangizo choyesera, ndiyeno yambani chowerengera. Pewani kutchera thovu la mpweya m'chitsime cha chitsanzo (S). Onani chithunzi pansipa.

3.Dikirani kuti mizere yamitundu iwonekere. Werengani zotsatira pa mphindi zisanu. Osamasulira zotsatira pakatha mphindi 10.

Mayendedwe a Test Strip

4. Bweretsani thumbalo kuti lizizizira bwino musanatsegule. Chotsani mzere woyesera m'thumba lomata ndikuchigwiritsa ntchito posachedwa.

5. Ndi mivi yoloza ku chitsanzo cha mkodzo, mizani mzere woyesera mumkodzo wa masekondi osachepera 10 mpaka 15.Miwiritsani mzerewo mpaka mulingo wa mizere yozungulira, koma osadutsa mzere wapamwamba (MAX) pamzere woyeserera. [Onani chithunzi (2)]

6. Ikani mzere woyesera pamalo athyathyathya osayamwa.

7. Yambitsani chowerengera ndikudikirira kuti mizere yamitundu iwonekere.

8. Zotsatira zake ziyenera kuwerengedwa mphindi zisanu. Zotsatira zitha kukhazikika pakatha ola limodzi mutangoyamba kuyesa.

Mayendedwe a Mayeso Khadi

1. Bweretsani thumbalo kuti lizizizira bwino musanatsegule. Chotsani khadi loyesera m'thumba losindikizidwa ndikuligwiritsa ntchito mwamsanga.

2.Chotsani kapu.

3.Ndi muvi woloza ku chitsanzo cha mkodzo, miza khadi yoyesera molunjika mu chitsanzo cha mkodzo kwa masekondi 10 mpaka 15.Miwiritsani mzerewo mpaka mulingo wa mizere yozungulira, koma osati pamwamba pa muvi pa khadi loyesera. [Onani chithunzi (1)]

4.Bwezerani kapu ndikuyika khadi yoyesera pamalo osa -

5.Yambani nthawi ndikudikirira kuti mizere yamitundu iwonekere.

6.Zotsatira ziyenera kuwerengedwa pa mphindi 5. Zotsatira zitha kukhazikika pakatha ola limodzi mutangoyamba kuyesa.