Ukuzwela nokucacisa: Idivayisi ka-2019-nCOV/COVID-19 IgG/IgM Rapid Test iqhathaniswe nokuhlola okuhamba phambili kwe-RT-PCR yezentengiselwano kusetshenziswa amasampula omtholampilo. Imiphumela ibonisa ukuthi i-2019-nCoV/COVID-19 IgG/IgM Rapid Test Device inokuzwela okuphezulu nokucacile.

Ngokuhlolwa kwe-IgG:

Ukuzwela Okuhlobene:233/268=86.94%(95%CI*:82.35%-90.49%)

Ukucaciswa Okuhlobene:287/289=99.31% (95%CI*:97.52%-99.92%)

Ukunemba:520/557=93.36% (95%CI*: 90.96%-95.16%)

*Isikhawu Sokuzethemba

Okokuhlolwa kwe-IgM

Ukuzwela Okuhlobene:223/268=83.21% (95%CI*:78.19%-87.48%)

Ukucaciswa okuhlobene:282/289=97.58% (95%CI*: 95.07%-99.02%)

Ukunemba: 505/557=90.66% (95%CI*: 87.94%-92.95%)

*Isikhawu Sokuzethemba

ISIMISO: Le kit isebenzisa i-immunochromatography.Ikhadi lokuhlola liqukethe:1) igolide le-colloidal-elilebulwe kabusha ukuthi i-coronavirus antigen kanye nezimpawu zegolide zokulawula i-antibody;2) imigqa emibili yokuthola (imigqa ye-IgG ne-IgM) kanye nomugqa owodwa wokulawula ikhwalithi (umugqa C) we-nitrocellulose membrane. inoveli coronavirus I-antibody ye-IgM; ulayini we-IgG awunyakazi nge-reagent yokuthola i-antibody ye-IgG yenoveli; futhi ulayini u-C awunyakazi ne-antibody elawula ikhwalithi. Uma inani elifanele lesampula lokuhlola lengezwa embotsheni yesampula yekhadi lokuhlola, isampula izoqhubekela phambili eduze nekhadi lokuhlola ngaphansi kwesenzo se-capillary.Uma isampula iqukethe i-antibody ye-IgM, i-antibody izobophezela ku-colloidal gold-ebhalwe inoveli coronavirus antigen. I-immune complex complex izothunjwa i-anti-human IgM antibody engakwazi ukuhamba kulwelwesi ukwenza ulayini we-IgM onsomi-obomvu, okubonisa ukuthi i-antibody ye-coronavirus ye-IgM ine-positive. Uma isampula iqukethe i-antibody ye-IgG, i-antibody izobophezela kugolide we-colloidal-okubhalwe ukuthi inoveli coronavirus antigen, futhi ukuzivikela komzimba kuzobanjwa ulwelwesi lwe-IgM ukuze lufakwe kabusha ulwelwesi lwe-IgG - umugqa, obonisa ukuthi inoveli coronavirus I-antibody ye-IgG i-positive.Uma imigqa yokuhlola ye-IgG ne-IgM ingenambala, kuvela umphumela ongemuhle.Ikhadi lokuhlola liqukethe nomugqa wokulawula ikhwalithi C.Umugqa wokulawula ikhwalithi we-fuchsia kufanele uvele kungakhathaliseki ukuthi umugqa wokuhlola uyavela yini.Umugqa wokulawula ikhwalithi uyibhande lombala lokulawula ikhwalithi i-antibody immune complex. Uma ulayini wokulawula ikhwalithi C ungaveli, umphumela wokuhlolwa awuvumelekile, futhi isampula idinga ukuhlolwa futhi ngelinye ikhadi lokuhlola.

Nakhu okudingeka ukwenze ukuze wenze ukuhlolwa:

1.Hlanza umunwe nge-alcohol swab.

2.Hlaba umunwe ofanayo ngomunwe ukuze uthole isampula yegazi.

3.Ukukhulula ikhava yenaliti jikisa imicibisholo elandela iwashi lesembozo.

4.Sebenzisa i-dropper ukudonsa igazi ukuze ulifake ekhasethini lokuhlola.

5.Hlola izimpawu ekhasethi ngemva kwemizuzu eyi-10 ukuze ubone umphumela.

ICoronavirus (CoV) iyingxenye yohlobo lwe-Nestovirus, i-Coronaviridae, futhi ihlukaniswe yaba izigaba ezintathu: α, β, kanye no-γ.Uhlobo α kanye no-β luyingozi kuphela ezilwaneni ezincelisayo.Uhlobo luka-γ lubangela izifo zezinyoni kakhulu. Kukhona nobufakazi bokuthi ingadluliselwa ngomzila we-fecal-oral.

Kuze kube manje, kunezinhlobo ezingu-7 ze-human coronavirus (HCoV) ezibangela izifo zokuphefumula komuntu: i-HCoV-229E,HCoV-OC43,SARS-CoV,HCoV-NL63,HCoV-HKU1,MERS-CoV kanye ne-novel coronaviruses (2019), iyigciwane elibalulekile lezifo zokuphefumula komuntu.Phakathi kwayo, kwatholakala i-coronavirus engu-201 emtholampilo. izimpawu zesistimu ezifana nemfiva nokukhathala, okuhambisana nokukhwehlela okomile kanye ne-dyspnea, njll., okungase kukhule ngokushesha kube inyumoniya enzima, ukwehluleka ukuphefumula, nokuphefumula kanzima.I-Distress syndrome, ukushaqeka kwe-septic, ukwehluleka kwezitho eziningi, i-asidi enzima-izinkinga ze-base metabolism, njll. zilingana nempilo-zisongela.

I-2019-nCOV/COVID-19 IgG/IgM Rapid Test Device iyi-chromatographic immunoassay esheshayo yokutholwa kwekhwalithi ye-IgG&IgM antibody ye-Coronavirus Disease 2019 egazini lomuntu lonke, i-serum, noma i-plasma njengosizo ekuxilongweni kwezifo ze-COVID-19.