【ZOFUNIKA KUGWIRITSA NTCHITO】

Chipangizo cha HSV 1/2 IgG/IgM Rapid Test Device ndi njira yofulumira kwambiri yodziŵikitsa matenda a chromatographic immunoassay yosonyeza mmene ma antibodies (IgG ndi IgM) amayendera (IgG ndi IgM) kupita ku Herpes Simplex Virus Type 1/2 mu Whole Blood/Serum/Plasma kuti athandize kuzindikira matenda a HSV.

【CHIDULE】

Herpes simplex virus (HSV) amagawidwa m'gulu la alpha virus m'banja la Herpesviridae. Mavairasi awiri ogwirizana-ogwirizana amatchulidwa kuti HSV mitundu 1 ndi 2. HSV-1 ndizomwe zimayambitsa matenda orolabial (gingivostomatitis kapena herpes labialis), pamene HSV-2 ndizomwe zimayambitsa matenda kumaliseche. Komabe, ma virus amatha kupatsira malo aliwonse. Pali njira zingapo zodziwira matenda a HSV.Serology ikhoza kukhazikitsa matenda amakono ndi akale ndi HSV. Zagwiritsidwanso ntchito pofufuza kafukufuku wa miliri ya HSV ndipo ndizothandiza kwambiri pazochitika zachilendo zachipatala. Kuyankha kwa antibody ku HSV glycoprotein G (IgG) ndikokhazikika kwambiri, ndipo kuyesa kwa IgG-kungathe kudziwa molondola ngati anthu adadwalapo kale ndi HSV-1 ndi/kapena HSV-2. Chifukwa matenda a HSV-2 amatha kuyambiranso kuposa matenda a HSV-1, kupezeka kwa antibody ku HSV-2 komanso mbiri yofananira yachipatala ungakhale umboni wamphamvu wotsimikizira kuti matendawa anali obwerezabwereza. Kuyeza kwa amayi apakati kuti apeze ma antibodies a HSV nthawi zambiri kumachitika ndi mtundu-kuyesa kwachindunji kwa ma antibodies a HSV.

【MALANGIZO OGWIRITSA NTCHITO】

Lolani kuyesa, chitsanzo, bafa ndi/kapena zowongolera kuti zifikire kutentha kwachipinda (15-30 ℃) musanayesedwe.

1.Bweretsani thumba kutentha kwa chipinda musanatsegule.Chotsani chipangizo choyesera kuchokera m'thumba losindikizidwa ndikuchigwiritsa ntchito mwamsanga.

2.Ikani Chipangizocho pamalo oyera komanso okwera.

l Za Seramu kapena Plasma chitsanzo:

Gwirani chotsitsacho molunjika ndikusamutsa 10ul seramu kapena madzi a m'magazi kudera la chitsanzo, kenaka yikani madontho awiri a buffer (pafupifupi 80μl), ndikuyamba chowerengera nthawi.Onani chithunzi pansipa.

l Zachitsanzo cha Venipuncture Whole Blood:

Gwirani chotsitsa molunjika ndikusamutsa magazi athunthu 20ul) kudera lachitsanzo, kenaka yikani madontho awiri a buffer (pafupifupi 80μl), ndikuyamba chowerengera nthawi.Onani chithunzi pansipa.

l Kwa chitsanzo cha Fingerstick Whole Blood:

Kugwiritsa ntchito chubu cha capillary: Dzazani chubu cha capillary ndikusamutsa ndodo 20ul yamagazi athunthu kumalo oyesera, kenaka yikani madontho awiri a buffer (pafupifupi 80μl) ndikuyamba chowerengera.

3.Dikirani kuti mizere yamitundu iwonekere. Werengani zotsatira pakadutsa mphindi 15. Osamasulira zotsatira pakadutsa mphindi 20.

Ndemanga:Kugwiritsa ntchito kuchuluka kokwanira kwachitsanzo ndikofunikira kuti mupeze zotsatira zolondola. Ngati kusamuka (kunyowetsa kwa nembanemba) sikukuwoneka pazenera loyesa pakatha mphindi imodzi, onjezerani dontho lina la buffer pachitsanzocho.