【AMFANI DA NUFIN】

Na'urar Gwajin Saurin H-FABP (Dukkan Jini/Magunguna/Plasma) shine saurin immunoassay na chromatographic don gano ingantacciyar H

【TAKAITACCEN】

zuciya Zuciya-nau'in Fatty Acid-

Binding Protein (H-FABP) ƙaramin furotin ne na cytoplasmic (15kDa) wanda aka saki daga myocytes na zuciya bayan yanayin ischemic.[2] Kamar sauran FABPs guda tara da aka gano, H - FABP yana shiga cikin metabolism na fatty acid mai aiki inda yake jigilar fatty acid daga membrane cell zuwa mitochondria don oxidation.

H - FABP ana ba da shawarar a auna shi tare da troponin don gano ciwon zuciya na zuciya da ciwon zuciya mai tsanani a cikin marasa lafiya da ke nuna ciwon kirji H - FABP da aka auna tare da troponin yana nuna ƙarar hankali na 20.6% akan troponin a 3-6 hours bayan ciwon kirji.[3]

Na'urar Gwajin Saurin H-FABP (Dukkan Jini/Serum/Plasma) gwaji ne mai sauƙi wanda ke amfani da haɗe-haɗe na anti-H- FABP antibody barbashi da kuma kama reagent don gano H-FABP a cikin jini gaba ɗaya, jini ko plasma.Maƙaman ganowa shine 5.0ng/mL.

【HANYOYIN AMFANI】

Bada gwajin, samfuri, buffer da/ko sarrafawa don isa zafin ɗaki (15-30°C) kafin gwaji.

1. Kawo jakar a cikin zafin jiki kafin buɗe shi. Cire na'urar gwaji daga jakar da aka rufe kuma amfani da ita cikin sa'a daya.

2. Sanya Na'urar a kan tsaftataccen wuri mai ma'auni.

DominSamfuran jini ko Plasma:

Rike digo a tsaye kumaCanja wurin digo 2 na jini ko plasma (kimanin 50μl) zuwa yankin samfurin, sannanƙara digo 1 na buffer (kimanin 40μl),kuma fara mai ƙidayar lokaci.Duba hoton da ke ƙasa.

DominSamfuran Jini Gabaɗayan Venipuncture:

Rike digo a tsaye kumacanja wurin digo guda 3 na dukkan jini (kimanin 75μl) zuwa yankin samfurin, sannanƙara digo 1 na buffer (kimanin 40μl),kuma fara mai ƙidayar lokaci.Duba hoton da ke ƙasa.

DominSamfurin Jini na Yatsa:

Don amfani da bututun capillary: Cika bututun capillary dacanja wuri kusan 75μl na ɗan yatsa cikakken samfurin jini zuwa yankin samfurin na'urar gwaji, sannanƙara digo 1 na buffer (kimanin 40μl) kuma fara mai ƙidayar lokaci. Dubi hoton da ke ƙasa.

3. Jira layin (s) masu launi ya bayyana.Karanta sakamako a minti 10.Kar a fassara sakamakon bayan mintuna 20.