【ZOYENERA KUGWIRITSA NTCHITO】

H-FABP Rapid Test Device (Whole Blood/Serum/Plasma) ndi kuyesa kofulumira kwa chromatographic immunoassay kwa munthu H-FABP m'magazi athunthu, seramu kapena plasma monga chothandizira pozindikira matenda a acute myocardial infarction (AMI).

【CHIDULE】

Mtima-mtundu wa mafuta asidi womanga mapuloteni (H-FABP) omwenso wotchedwa mammary-derived growth inhibitor ndi mapuloteni amene mwa anthu amapangidwa ndi jini ya FABP3.[1] Mtima- Type Fatty Acid-

Kumanga Mapuloteni (H-FABP) ndi puloteni yaying'ono ya cytoplasmic (15kDa) yotulutsidwa kuchokera ku mtima wa myocyte kutsatira ischemic episode.[2] Monga ma FABP ena asanu ndi anayi odziwika bwino omwe azindikirika, H-FABP imakhudzidwa ndi kagayidwe ka mafuta a acid komwe imanyamula mafuta acids kuchokera ku cell membrane kupita ku mitochondria kuti atenge okosijeni.[2]

H - FABP ikulimbikitsidwa kuti iyesedwe ndi troponin kuti izindikire infarction ya myocardial ndi matenda aakulu a mtima kwa odwala omwe amamva kupweteka pachifuwa H - FABP yoyesedwa ndi troponin imasonyeza kuwonjezeka kwa 20.6% pa troponin pa maola 3-6 pambuyo pa kupweteka pachifuwa.

H-FABP Rapid Test Device (Whole Blood/Serum/Plasma) ndi kuyesa kosavuta komwe kumagwiritsa ntchito tinthu tating'ono ta anti-H-FABP takutidwa ndi antibody ndi capreagent kuti izindikire H-FABP m'magazi athunthu, seramu kapena plasma. Mulingo wocheperako ndi 5.0ng/mL.

【MALANGIZO OGWIRITSA NTCHITO】

Lolani kuyesa, zitsanzo, zotchingira ndi/kapena zowongolera zifikire kutentha kwachipinda (15-30°C) musanayesedwe.

1. Bweretsani thumbalo kuti lizizizira musanatsegule. Chotsani chipangizo choyesera m'thumba lomata ndipo chigwiritseni ntchito mkati mwa ola limodzi.

2. Ikani Chipangizocho pamalo oyera komanso osalala.

ZaSeramu kapena Plasma chitsanzo:

Gwirani chotsitsacho molunjika ndikusamutsa madontho awiri a seramu kapena plasma (pafupifupi 50).μl) kudera lachitsanzo, ndiyeonjezani dontho limodzi la buffer (pafupifupi 40μl),ndipo yambani chowerengera nthawi.Onani chithunzi pansipa.

ZaVenipuncture Magazi Athunthu:

Gwirani chotsitsacho molunjika ndikusamutsa madontho atatu amagazi athunthu (pafupifupi 75).μl) kudera lachitsanzo, ndiyeonjezani dontho limodzi la buffer (pafupifupi 40μl),ndipo yambani chowerengera nthawi.Onani chithunzi pansipa.

ZaChitsanzo cha Fingerstick Whole Blood:

Kugwiritsa ntchito chubu cha capillary: Dzazani chubu cha capillary ndikusamutsa pafupifupi 75μl chala chala magazi athunthu kupita kugawo lachitsanzo la chipangizo choyesera, ndiyeonjezani dontho limodzi la buffer (pafupifupi 40μl) ndi kuyambitsa chowerengera. Onani chithunzi pansipa.

3. Dikirani kuti mizere yachikuda iwonekere.Werengani zotsatira pa mphindi khumi.Osamasulira zotsatira pakatha mphindi 20.