【TŠEBELETSO TŠEBELETSO】

H-FABP Rapid Test Device (Whole Blood/Serum/Plasma) ke tlhahlobo e potlakileng ea chromatographic immunoassay bakeng sa ho lemoha boleng ba motho H-FABP maling a felletseng, serum kapa plasma e le thuso bakeng sa tlhahlobo ea acute myocardial infarction (AMI).

【KAKARETSO】

Pelo-mofuta mafura asiti e tlamang protheine (H-FABP) eo hape e tsejoang e le mammary-derived growth inhibitor ke protheine eo bathong e kentsoeng ke lefutso la FABP3.[1] Pelo- mofuta oa Fatty Acid-

Ho Tlama Protein (H-FABP) ke protheine e nyane ea cytoplasmic (15kDa) e tsoang ho myocyte ea pelo ka mor'a ketsahalo ea ischemic.[2] Joalo ka li-FABP tse ling tse robong tse khetholloang, H-FABP e ameha ho metabolism e sebetsang ea asiti moo e tsamaisang mafura a acid ho tloha lera la sele ho ea ho mitochondria bakeng sa oxidation.[2]

H - FABP e kgothaletswa ho lekanyetswa ka troponin ho tsebahatsa infarction ea myocardial le acute coronary syndrome ho bakuli ba hlahisang bohloko ba sefuba H - FABP e lekantsoeng ka troponin e bontša kutloisiso e eketsehileng ea 20.6% ho feta troponin ka lihora tsa 3-6 ka mor'a hore bohloko ba sefuba bo qale. [3]

H-FABP Rapid Test Device (Whole Blood/Serum/Plasma) ke teko e bonolo e sebedisang motswako wa anti-H-FABP antibody coated particles and capture reagent ho lemoha H-FABP maling kaofela, serum kapa plasma.Bonyane boemo ba ho lemoha ke 5.0ng/mL.

【LITS'ELISO TŠEBELETSO】

Dumella teko, mohlala, buffer le/kapa ditaolo ho fihlela mocheso wa kamore (15-30°C) pele ho teko.

1. Tlisa mokotla mochesong oa kamore pele u o bula.Tlosa sesebelisoa sa teko ka mokotleng o koetsoeng 'me u se sebelise nakong ea hora e le' ngoe.

2. Beha Sesebelisoa sebakeng se hloekileng le se lekaneng.

Bakeng saMohlala oa Serum kapa Plasma:

Tšoara dropper ka holimo lefetisetsa marotholi a 2 a serum kapa plasma (hoo e ka bang 50).μl) ho ea sebakeng sa mohlala, joaleeketsa lerotholi le le leng la buffer (hoo e ka bang 40μl),'me u qale nako.Sheba setšoantšo se ka tlase.

Bakeng saVenipuncture Whole Blood specimen:

Tšoara dropper ka holimo lefetisetsa marotholi a 3 a mali a felletseng (hoo e ka bang 75).μl) ho ea sebakeng sa mohlala, joaleeketsa lerotholi le le leng la buffer (hoo e ka bang 40μl),'me u qale nako.Sheba setšoantšo se ka tlase.

Bakeng saMohlala oa Fingerstick Whole Blood:

Ho sebelisa tube ea capillary: Tlatsa tšupu ea capillary lefetisetsa hoo e ka bang 75μl ea menoana ea mali a felletseng sebakeng sa mohlala oa sesebelisoa sa tlhahlobo, joaleeketsa lerotholi le le leng la buffer (hoo e ka bang 40μl) ebe o qala sebali sa nako. Sheba setšoantšo se ka tlase.

3. Emela hore mela e mebala e hlahe.Bala liphetho ka metsotso e 10.Se ke oa toloka sephetho kamora metsotso e 20.