【UKUSETYENZISWA OKUHLOSIWEYO】

I-H-FABP Rapid Test Device (Igazi Elilonke/iSerum/Plasma) luvavanyo olukhawulezayo lwe-chromatographic immunoassay yokufumanisa umgangatho womntu we-H-FABP kwigazi elipheleleyo, i-serum okanye i-plasma njengoncedo ekuxilongweni kwe-acute myocardial infarction (AMI).

【USHWANKATHELO】

Intliziyo-uhlobo amafutha asidi ebopha iprotheyini (H-FABP) ekwabizwa ngokuba njenge mammary-derived growth inhibitor yiprotheyini efakwa ebantwini yi-FABP3 gene.[1] Intliziyo-chwetheza i-Fatty Acid-

Iprotheyini ebophelelayo (H - FABP) yiprotheni encinci ye-cytoplasmic (15kDa) ekhutshwe kwi-myocytes ye-cardiac elandela i-ischemic episode.[2] Njengezinye ii-FABPs ezisithoba ezichongiweyo, i-H-FABP ibandakanyeka kwi-fatty acid metabolism esebenzayo apho ithutha i-acids enamafutha ukusuka kwi-cell membrane ukuya kwi-mitochondria ye-oxidation.[2]

I-H - i-FABP inconywa ukuba ilinganiswe ne-troponin ukuchonga i-myocardial infarction kunye ne-acute coronary syndrome kwizigulane ezibonisa intlungu yesifuba H - i-FABP elinganiswe ne-troponin ibonisa uvakalelo olwandisiweyo lwe-20.6% ngaphezu kwe-troponin kwiiyure ze-3-6 emva kokuqala kweentlungu zesifuba.

I-H-FABP Rapid Test Device (Igazi Elipheleleyo/iSerum/Plasma) luvavanyo olulula olusebenzisa indibaniselwano ye-anti-H-FABP antibody coated amasuntswana kunye ne-reagent yokubamba ukubona i-H-FABP kwigazi elipheleleyo, i-serum okanye i-plasma.Umgangatho wokufumanisa ubuncinci yi-5.0ng/mL.

【IZIKHOKELO ZOKUSETYENZISWA】

Vumela uvavanyo, umfuziselo, isithinteli kunye/okanye nezilawuli ukufikelela kubushushu begumbi (15-30°C) phambi kovavanyo.

1. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokusivula.Susa isixhobo sovavanyo kwisingxobo esitywiniweyo kwaye usisebenzise kwiyure enye.

2. Beka iDivaysi kwindawo ecocekileyo kunye nenqanaba.

KubaIsampulu yeSerum okanye yePlasma:

Bamba idropha ngokuthe nkqo kwayeukudlulisa ama-2 amathontsi e-serum okanye i-plasma (malunga ne-50μl) ukuya kwindawo yomzekelo, ngokoyongeza ithontsi enye yesithinteli (malunga nama-40μl),kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

KubaIVenipuncture yeGazi Elipheleleyo:

Bamba idropha ngokuthe nkqo kwayeUkutshintshela amathontsi ama-3 egazi elipheleleyo (malunga nama-75μl) ukuya kwindawo yomzekelo, ngokoyongeza ithontsi enye yesithinteli (malunga nama-40μl),kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

KubaUmnwe weGazi Elipheleleyo:

Ukusebenzisa ityhubhu ye-capillary: Gcwalisa ityhubhu ye-capillary kunyeukutshintshela malunga nama-75μl yeminwe yomfanekiso wegazi elipheleleyo ukuya kwindawo yomzekelo wesixhobo sovavanyo, ngokoyongeza ithontsi enye yesithinteli (malunga nama-40μl) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.

3. Lindela umgca onemibala ukuba uvele.Funda iziphumo ngemizuzu eyi-10.Musa ukutolika umphumo emva kwemizuzu engama-20.