【Ezubere iji】

Ngwa ule H-FABP ngwa ngwa (Ọbara dum/Serum/Plasma) bụ ngwa ngwa immunoassay chromatographic maka nchọpụta qualitative nke mmadụ H-FABP n'ọbara dum, serum ma ọ bụ plasma dị ka ihe enyemaka na nchọpụta nke nnukwu myocardial infarction (AMI).

【Nchịkọta】

Obi-ụdị fatty acid binding protein (H-FABP) a marakwa mammary- ihe mgbochi ụtara bụ protein nke mmadụ bụ mkpụrụ ndụ FABP3 kpuchiri.[1] Obi-ụdị Fatty Acid-

Bing Protein (H Dị ka FABP ndị ọzọ dị iche iche nke achọpụtara, H - FABP na-etinye aka na fatty acid metabolism ebe ọ na-ebufe acid fatty si na cell membrane na mitochondria maka oxidation.

H - FABP na-atụ aro ka a tụọ ya na troponin iji chọpụta infarction myocardial na nnukwu ọrịa obi na-arịa ọrịa obi na ndị ọrịa na-enwe mgbu obi H - FABP tụrụ na troponin na-egosi mmụba nke 20.6% karịa troponin na 3-6 awa na-esote mgbu obi malitere.[3]

Ngwaọrụ nnwale ngwa ngwa H-FABP (Ọbara dum/Serum/Plasma) bụ nnwale dị mfe nke na-eji ngwakọta mgbochi - H- FABP antibody kpuchie ma weghara reagent iji chọpụta H-FABP n'ọbara dum, serum ma ọ bụ plasma. Ọkwa nchọpụta kacha nta bụ 5.0ng/mL.

【Ntuziaka maka ojiji】

Kwe ka nnwale ahụ, ihe nlere, ihe nchekwa na/ma ọ bụ njikwa ka ọ ruo n'ime ụlọ (15-30°C) tupu nnwale.

1. Weta obere akpa ahụ n'ime ụlọ okpomọkụ tupu imeghe ya. Wepu ngwaọrụ ule na obere akpa ahụ mechiri emechi ma jiri ya n'ime otu awa.

2. Debe ngwaọrụ ahụ n'elu ala dị ọcha ma dị larịị.

MakaSerum ma ọ bụ Plasma:

Jide dobe ahụ kwụ ọtọ naNyefee 2 tụlee nke serum ma ọ bụ plasma (ihe dị ka 50μl) gaa na mpaghara ihe atụ, mgbe ahụtinye 1 dobe nchekwa (ihe dị ka 40μl),ma malite ngụ oge.Lee ihe atụ n'okpuru.

MakaỤdị Ọbara Nile Venipuncture:

Jide dobe ahụ kwụ ọtọ nabufee 3 nke ọbara dum (ihe dịka 75μl) gaa na mpaghara ihe atụ, mgbe ahụtinye 1 dobe nchekwa (ihe dị ka 40μl),ma malite ngụ oge.Lee ihe atụ n'okpuru.

MakaNlere ọbara dum mkpịsị aka:

Iji jiri tube capillary: Jupụta tube capillary nanyefee ihe dịka 75μl nke mkpịsị aka ọbara dum ruo n'akụkụ ihe atụ nke ngwaọrụ nnwale, mgbe ahụtinye 1 dobe nchekwa (ihe dị ka 40μl) ma malite ngụ oge. Lee ihe atụ n'okpuru.

3. Chere ka ahịrị(s) nwere agba pụta.Gụọ nsonaazụ na nkeji iri.Atụgharịla nsonaazụ ya mgbe nkeji iri abụọ gachara.