Atụle uche na nkọwapụta: Ihe 2019-nCOV/COVID-19 IgG/IgM Ngwa ule ngwa ngwa ka atụnyere nleba anya azụmahịa RT-Nnwale PCR site na iji ụdị ụlọ ọgwụ. Nsonaazụ gosiri na 2019-nCoV/COVID-19 IgG/IgM Ngwa ule ngwa ngwa nwere ọgụgụ isi na nkọwapụta.

Maka ule IgG:

Mmetụta mmetụta:233/268=86.94%(95%CI*:82.35%-90.49%)

Nkọwa nkọwa:287/289=99.31% (95%CI*:97.52%-99.92%)

Nzizi:520/557=93.36% (95%CI*: 90.96%-95.16%)

* Ogologo oge ntụkwasị obi

Maka ule IgM

Mmetụta mmetụta:223/268=83.21% (95%CI*:78.19%-87.48%)

Nkọwa nkọwa:282/289=97.58% (95%CI*: 95.07%-99.02%)

Nzizi: 505/557=90.66% (95%CI*: 87.94%-92.95%)

* Ogologo oge ntụkwasị obi

Ngwa 2019-nCOV/COVID-19 IgG/IgM Ngwa ngwa ngwa ngwa bụ immunoassay chromatographic ngwa ngwa maka nchọpụta qualitative nke IgG&IgM antibody nke Coronavirus Ọrịa 2019 n'ime ọbara mmadụ dum, serum, ma ọ bụ plasma dị ka ihe enyemaka na nchoputa nke COVID-19 ọrịa.

Ezubere iji: Ngwa 2019-nCOV/COVID-19 IgG/IgM Ngwa ule ngwa ngwa bụ immunoassay ngwa ngwa chromatographic maka nchọpụta qualitative nke IgG&IgM antibody nke Coronavirus Ọrịa 2019 n'ime ọbara mmadụ dum, serum, ma ọ bụ plasma dị ka ihe enyemaka na nchoputa nke COVID-19 ọrịa.

NKWUO: COVID-19 bụ nnukwu ọrịa na-efe efe nke iku ume. Ndị mmadụ na-adịkarị mfe. Ugbu a, ndị ọrịa bu ọrịa coronavirus ọhụrụ bụ isi ihe na-ebute ọrịa; ndị butere ọrịa asymptomatic nwekwara ike ịbụ isi iyi na-efe efe. Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1 ruo 14, ọkachasị ụbọchị 3 ruo 7. Ihe ngosi ndị bụ isi gụnyere ahụ ọkụ, ike ọgwụgwụ na ụkwara akọrọ. A na-ahụ mkpọchi imi, imi na-agba agba, akpịrị akpịrị, myalgia na afọ ọsịsa n'ọnọdụ ole na ole.

Ntuziaka maka ojiji: Kwe ka ngwaọrụ nnwale, ụdị, ihe nchekwa na/ma ọ bụ njikwa rute ọnụ ụlọ okpomọkụ (15-30°C) tupu nnwale. 1. Weta obere akpa ahụ na okpomọkụ tupu imeghe. Wepu ngwaọrụ nnwale ahụ na obere akpa mkpuchi ma jiri ya ozugbo enwere ike. 2. Debe ngwaọrụ ule na elu dị ọcha na larịị. ? Maka ihe nleba anya nke ọbara ma ọ bụ Plasma: Iji pipette 10μL a na-atụfu ihe, dọpụta ihe nlele ahụ ruo Line Fill, wee bufee 10μL serum/plasma n'ụdị nke ọma nke ngwaọrụ nnwale, wee tinye 2 tụlee nke ihe nchekwa wee malite ngụ oge. ? Maka Ọbara dum (Venipuncture/Fingerstick) Ihe atụ: Iji 10μL nke a na-atụfu pipette, ma nyefee 1 dobe ọbara dum (ihe dị ka 20μL) na ụdị nke ọma nke ngwaọrụ ule, wee tinye 2 tụlee nke ihe nchekwa wee malite ngụ oge. Mara: Enwere ike itinye ihe nlele site na iji micropipette. 3. Chere ka ahịrị (s) na-acha acha pụta. Gụọ nsonaazụ na nkeji 10. Atụgharịla nsonaazụ mgbe nkeji iri na ise gachara.

Ụkpụrụ: Ngwa a na-eji immunochromatography. Kaadị ule ahụ nwere: 1) colloidal gold-akpọrọ recombinant novel coronavirus antigen na àgwà akara ihe nrịbama ọla edo; 2) ahịrị nchọpụta abụọ (IgG na IgM Line) na otu akara njikwa (C line) nke akpụkpọ ahụ nitrocellulose. Eji monoclonal mgbochi - mgbochi IgM mmadụ maka ịchọpụta ihe mgbochi coronavirus ọhụrụ IgM; A na-eji ihe reagent eme ka ahịrị IgG megharịa maka ịchọpụta ihe mgbochi coronavirus IgG ọhụrụ; na ahịrị C anaghị emegharị ahụ site na iji mgbochi njikwa mma. Mgbe agbakwunyere ego kwesịrị ekwesị nke ihe nlele ule na oghere nlele nke kaadị ule ahụ, ihe nlele ahụ ga-aga n'ihu na kaadị ule n'okpuru ọrụ nke capillary. Ọ bụrụ na ihe nlele ahụ nwere ihe mgbochi IgM, ihe mgbochi ahụ ga-ejikọta na colloidal gold-akpọrọ aha coronavirus antigen. A ga-ejide ogige ahụ mgbochi ahụ site na mgbochi - mmadụ IgM mgbochi na-enweghị ike ime na akpụkpọ ahụ iji mepụta ahịrị na-acha odo odo - Red M, na-egosi na akwụkwọ akụkọ coronavirus IgM antibody dị mma. Ọ bụrụ na ihe nlele ahụ nwere mgbochi IgG, mgbochi ahụ ga-ejikọta ya na ọla edo colloidal nke akpọrọ coronavirus antigen, na ihe mgbochi ahụ ga-ejide ya site na reagent immobilized na akpụkpọ ahụ iji mepụta ahịrị IgG na-acha odo odo, na-egosi na akwụkwọ akụkọ coronavirus IgG antibody dị mma. Ọ bụrụ na ule IgG na IgM ahịrị anaghị agba agba, a na-egosipụta nsonaazụ na-adịghị mma. Kaadị ule nwekwara akara njikwa mma C. The fuchsia quality control akara C kwesịrị ịpụta n'agbanyeghị ma akara ule pụtara. Ọ bụrụ na ahịrị njikwa mma C egosighi, nsonaazụ nnwale ahụ abaghị uru, yana ekwesịrị iji kaadị ule ọzọ nwalee ihe nlele ahụ ọzọ.