【Ezubere iji】

NT-proBNP Ngwa ngwa ule ngwa ngwa (Colloidal Gold) bụ nnwale diagnostic in vitro maka mkpebi qualitative nke ntinye uche nke N-terminal pro-Brain Natriurtic Peptide na ọbara mmadụ,plasma ma ọ bụ ọbara dum.

【Nchịkọta】

NT-proBNP tumadi na-abịa site ventricular myocytes. Mgbe aka ekpe ventricular olu overloads ma ọ bụ ike nke contraction mmụba,proBNP, na cardiogenic prohormone, na-ewepụtara, decomposed n'ime 76 amino acid mejupụtara NT-proBNP na biologically arụ ọrụ NT nchọpụta nke NT-proBNP nwere ike inye ntụaka siri ike maka nyocha ụlọ ọgwụ, ọgwụgwọ na nyocha amụma maka nchọpụta ọrịa obi mgbagha, NT-proBNP nwere ogologo ọkara - ndụ n'ime awa 2, mmụba ọbara dị elu (ihe dị ka ugboro 15-20 karịa ntinye nke BNP) n'ime ọbara. A ga-echekwa ya ụbọchị asaa n'ime ụlọ okpomọkụ, ruo ọtụtụ ọnwa na - 20 ℃.

Nchọpụta nke nkụda obi: ọnụ ọgụgụ dị ukwuu nke nyocha ụlọ ọgwụ egosila, NT-proBNP na-enwe mmetụta nke ukwuu n'ịchọpụta nkwụsị nke ventricular aka ekpe, itinye uche n'ọbara ya nwere njikọ chiri anya na ịdị njọ nke nkụda obi, bụ ihe dị mkpa ụlọ ọgwụ maka ịkọ ihe ize ndụ nke nkụda mmụọ obi, ịkọ ọnwụ obi mberede na mberede, na nlekota ọgwụgwọ na prognosis nke ọdịda obi. Ka ọ dị ugbu a, ebe ọ bụ na ihe karịrị 50% nke nnukwu nsogbu iku ume nwere ike ịgwọta dị ka nkụda mmụọ obi n'ụlọ ọgwụ n'agbanyeghị na ọ bụghị nkụda mmụọ kpatara ya, yabụ nchọta NT-proBNP bụ ihe dị mkpa n'ụlọ ọgwụ gbasara obi na enweghị -

Ọgwụgwọ na Nyocha Nyocha: NT - Nnwale proBNP bụ nnukwu enyemaka na nyocha nke nsonaazụ onye ọrịa na nhazi nke ọgwụgwọ ahụ. Ka ọ dị ugbu a, nchọpụta nke NT - proBNP nwere ike inye aka nyochaa prognosis nke ndị ọrịa nwere obi mgbawa. Nnyocha egosiwo na ndị ọrịa nwere obi mgbawa nwere ezigbo prognosis mgbe ntinye ọbara nke NT - proBNP na-ebelata ihe karịrị 30% mgbe ọgwụgwọ gasịrị.

【Ntuziaka maka ojiji】

Kwe ka ule ahụ, ihe nlere anya, ihe nchekwa na/ma ọ bụ njikwa ka ọ rute ọnụ ụlọ okpomọkụ (15-30)℃) tupu anwale.

1.Weta ihe niile na ihe atụ na okpomọkụ ụlọ.

2.Wepụ ngwaọrụ ule ahụ site na akpa foil nke mechiri emechi, tinye ebe dị ọcha, ọkwa ọkwa.

3. Tinye ngwaọrụ ahụ n'elu ala dị ọcha ma dị larịị.

Makaserum na/ma ọ bụ plasma ụdị:

Jide dobe ahụ kwụ ọtọ naNyefee 2 tụlee nke plasma ma ọ bụ serum (ihe dị ka 50 μl) gaa na mpaghara ihe atụ, mgbe ahụtinye 1 dobe nchekwa (ihe dị ka 40μl),ma malite ngụ oge.

MakaỤdị Ọbara Nile Venipuncture:

Jide dobe ahụ kwụ ọtọ nanyefee 2 tụlee ọbara dum (ihe dị ka 50μl)gaa na mpaghara ihe atụ, mgbe ahụ tinye 2 tụlee nke ihe nchekwa (ihe dị ka 80μl),ma malite ngụ oge.Lee ihe atụ n'okpuru.

MakaNlere ọbara dum mkpịsị aka:

Ijitube nke capillaries:Jujuo tube capillary wee bufee ihe dị ka 50μl nke mkpịsị aka mkpịsị aka n'ọbara niile n'akụkụ ihe nlele ngwaọrụ, wee tinye 2 tụlee nke ihe nchekwa (ihe dị ka 80μl) wee malite ngụ oge. Lee ihe atụ n'okpuru.

Ijitụlee okwute:Kwe ka mkpọda ntụtụ abụọ n'ụkpụrụ ọbara dum

(ihe dị ka 50μl) ịdaba na mpaghara ihe nlele ngwaọrụ, tinyezie 2 tụlee nke ihe nchekwa (ihe dị ka 80μl) wee malite ngụ oge.

4.Chere ka band (s) nwere agba pụta.Ekwesịrị ịgụ nsonaazụ ya na nkeji iri na ise.Atụgharịla nsonaazụ ya mgbe nkeji iri abụọ gachara.