【UKUSETSHENZISWA OKUHLOSIWE】

I-Ethyl Glucuronide (ETG) Rapid Test (Urine) iyisivivinyo esisheshayo se-chromatographic immunoassay ukuze kutholwe i-Ethyl Glucuronide emchameni womuntu. I-Ethyl Glucuronide etholwe ukuhlolwa ihlanganisa, kodwa ayikhawulelwe, kuma-metabolites e-Ethanol.

Lesi sivivinyo sinikeza umphumela wokuqala wokuhlaziya kuphela. Enye indlela yamakhemikhali ecacile kufanele isetshenziswe ukuze kutholwe umphumela wokuhlaziya oqinisekisiwe. I-Gas chromatography/mass spectrometry (GC/MS) iyindlela yokuqinisekisa ekhethwayo. Ukucatshangelwa komtholampilo kanye nokwahlulela kochwepheshe kufanele kusetshenziswe kunoma yimuphi umphumela wokuhlolwa kokuhlukumeza, ikakhulukazi uma kusetshenziswa imiphumela yokuqala emihle.

【ISIFINYEZO】

I-Ethyl Glucuronide (ETG) iyi-metabolite ye-ethyl alcohol eyakhiwa emzimbeni ngeglucuronidation ngemva kokuchayeka ku-ethanol, njengokuphuza utshwala. ochwepheshe, abameli, abashayeli bezindiza abalulama emiluthweni), ezikoleni, emitholampilo yokufakelwa kwe-inliver, noma ekutholeni iziguli ezidakiwe.ETG ingakalwa ngomchamo ngezindlela zaselabhorethri yeSintu (GC/MS noma LC/MS) kuze kufike emahoreni angaba ngu-80 ngemva kokuba i-ethanol idliwe (okuncike kakhulu enanini lotshwala nokungewona ophuzweni). I-ETG iyinkomba enembe kakhudlwana yokuchayeka lapho otshwaleni kunokulinganisa ukuba khona kwe-ethanol ngokwayo.

I-Ethyl Glucuronide Rapid Test (Umchamo) ukuhlolwa komchamo okusheshayo okungenziwa ngaphandle kokusebenzisa ithuluzi. Ukuhlola kusebenzisa i-antibody ye-monoclonal ukuthola ngokukhetha amazinga aphakeme e-ethyl glucuronide emchameni womuntu. I-Ethyl Glucuronide Rapid Test Strip (Umchamo) ikhiqiza umphumela omuhle lapho i-Ethyl Glucuronide emchameni idlula u-500 ng/mL.

【IZINDLELA ZOKUSEBENZISA】

Vumela ukuhlolwa, isifanekiso somchamo, kanye/noma izilawuli ukuthi zifinyelele izinga lokushisa legumbi (15-30ºC) ngaphambi kokuhlolwa.

1.Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula. Khipha idivayisi yokuhlola esikhwameni esivaliwe bese uyisebenzisa phakathi nehora elilodwa.

2.Beka idivayisi yokuhlola endaweni ehlanzekile nesezingeni. Bamba i-dropper ngokuqondile bese udlulisela amathonsi angu-3 agcwele omchamo (cishe 120ul) esibonelweni somthombo (S) weDivaysi yokuhlola, bese uqala isibali sikhathi. Gwema ukubamba amabhamuza omoya emthonjeni wesifanekiso (S). Bheka umfanekiso ongezansi.

3.Linda ukuthi kuvele ulayini onemibala. Funda imiphumela ngemizuzu emi-5. Ungahumushi umphumela ngemuva kwemizuzu eyi-10.

Izikhombisi-ndlela ze-Test Strip

4. Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula. Susa umugqa wokuhlola esikhwameni esivaliwe futhi uyisebenzise ngokushesha ngangokunokwenzeka.

5. Ngemicibisholo ebheke esibonelweni somchamo, cwilisa umugqa wokuhlola uqonde esibonelweni somchamo okungenani imizuzwana eyi-10 kuya kweyi-15.Gxilisa umucu okungenani ezingeni lemigqa yamagagasi, kodwa ungadluli umugqa ophezulu (MAX) emgqeni wokuhlola. [Bheka isithombe (2)]

6. Beka umugqa wokuhlola endaweni eyisicaba engamubi.

7. Qala isibali sikhathi bese ulinda ukuthi kuvele ulayini/amalayini anombala.

8. Umphumela kufanele ufundwe ngemizuzu emi-5. Imiphumela ingase izinze kufikela ehoreni elingu-1 ngemva kokuqaliswa kokuhlolwa.

Izikhombisi-ndlela zekhadi lokuhlola

1. Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula. Susa ikhadi lokuhlola esikhwameni esivaliwe futhi ulisebenzise ngokushesha okukhulu.

2.Susa ikepisi.

3.Ngomcibisholo obheke esibonelweni somchamo, cwilisa ikhadi lokuhlola liqonde esibonelweni somchamo okungenani imizuzwana eyi-10 kuya kweyi-15.Gxilisa umucu okungenani ezingeni lemigqa yamagagasi, kodwa hhayi ngaphezu komcibisholo ekhadini lokuhlola. [Bheka isithombe (1)]

4.Buyisela isivalo bese ubeka ikhadi lokuhlola endaweni eyisicaba engamumuki.

5.Qala isibali sikhathi bese ulinda ukuthi kuvele ulayini/amalayini anombala.

6.Umphumela kufanele ufundwe ngemizuzu emi-5. Imiphumela ingase izinze kufikela ehoreni elingu-1 ngemva kokuqaliswa kokuhlolwa.