【Ezubere iji】

Ethyl Glucuronide (ETG) Nnwale ngwa ngwa (Mmiri) bụ ngwa ngwa immunoassay chromatographic maka nchọpụta Ethyl Glucuronide na mmamịrị mmadụ. Ethyl Glucuronide nke nnwale ahụ chọpụtara gụnyere, mana ọnweghị oke na, metabolites nke Ethanol.

Nnwale a na-enye naanị nsonaazụ nyocha mbido. A ghaghị iji usoro kemịkalụ ọzọ akọwapụtara nke ọma iji nweta nsonaazụ nyocha enwetara. Gas chromatography/ mass spectrometry (GC/MS) bụ usoro nkwenye kachasị mma. Ekwesịrị itinye nleba anya ụlọ ọgwụ na ikpe ọkachamara na nsonaazụ nnwale ọgwụ ọ bụla, ọkachasị mgbe ejiri nsonaazụ izizi izizi.

【Nchịkọta】

Ethyl Glucuronide (ETG) bụ metabolite nke mmanya ethyl nke a na-etolite n'ime ahụ site na glucuronidation na-esote ikpughe na ethanol, dị ka ịṅụ mmanya na-aba n'anya. Addictions), n'ụlọ akwụkwọ, ụlọ ọgwụ transplant inliver, ma ọ bụ na-agbake ndị ọrịa aṅụrụma.ETG nwere ike tụọ ya na mmamịrị site na usoro ụlọ nyocha omenala (GC/MS ma ọ bụ LC/MS) ruo ihe dị ka awa 80 mgbe a gbasịrị ethanol (ọ dabere na ọnụọgụ mmanya na-aba n'anya na ndị na-abụghị mmanya). ETG bụ ihe ngosipụta ziri ezi nke ikpughe mmanya na-aba n'anya karịa ịtụ maka ọnụnọ ethanol n'onwe ya.

Ethyl Glucuronide Rapid Test (Urine) bụ ule nyocha mmamịrị ngwa ngwa nke enwere ike ịme na-ejighi ngwa. Nnwale ahụ na-eji ọgwụ mgbochi monoclonal were họrọ ịchọpụta ọkwa dị elu nke ethyl glucuronide na mmamịrị mmadụ. Ethyl Glucuronide Rapid Test Strip (Mmiri) na-arụpụta ezigbo nsonaazụ mgbe Ethyl Glucuronide na mmamịrị karịrị 500 ng/mL.

【Ntuziaka maka ojiji】

Kwe ka nnwale ahụ, ụdị mmamịrị, na/ma ọ bụ njikwa ka ọ rute n'ime ụlọ (15-30ºC) tupu anwale.

1.Weta obere akpa ahụ na okpomọkụ tupu imeghe ya. Wepu ngwaọrụ nnwale ahụ na obere akpa ahụ mechiri emechi wee jiri ya n'ime otu awa.

2. Tinye ngwaọrụ ule n'elu ala dị ọcha ma dị larịị. Jide dropper kwụ ọtọ ma bufee mmamịrị 3 zuru ezu (ihe dị ka 120ul) n'ụdị nke ọma (S) nke ngwaọrụ nnwale, wee malite ngụ oge. Zere ọnyà ikuku n'ime olulu mmiri (S). Lee ihe atụ a n'okpuru.

3.Chere ka ahịrị (s) nwere agba pụta. Gụọ nsonaazụ na nkeji ise. Atụgharịla nsonaazụ ya mgbe nkeji iri gachara.

Ntuziaka maka eriri ule

4. Weta obere akpa ahụ na okpomọkụ tupu imeghe ya. Wepu mpempe ule na obere akpa ahụ mechiri emechi wee jiri ya ozugbo enwere ike.

5. N'iji akụ na-atụ aka n'ụdị mmamịrị, tinye eriri ule ahụ kwụ ọtọ n'ime ụdị mmamịrị ma ọ dịkarịa ala 10 ruo 15 sekọnd.Na-emikpu warara ahụ ka ọ dịkarịa ala ọkwa nke ahịrị wavy, mana agabigala ahịrị kacha elu (MAX) na mpempe ule. [Lee foto (2).]

6. Debe eriri ule ahụ n'elu ala adịghị mma -

7. Malite ngụ oge wee chere ka ahịrị (s) nwere agba pụta.

8. Ekwesịrị ịgụ nsonaazụ ya na nkeji 5. Nsonaazụ nwere ike kwụsie ike ruo elekere 1 ka mmalite ule gachara.

Ntuziaka maka kaadị ule

1. Weta obere akpa ahụ na okpomọkụ tupu imeghe ya. Wepu kaadị ule ahụ na obere akpa mkpuchi ma jiri ya ozugbo enwere ike.

2.Wepu okpu.

3.N'iji akụ na-atụ aka na ụdị mmamịrị ahụ, gbanye kaadị ule ahụ kwụ ọtọ n'ime ụdị mmamịrị ma ọ dịkarịa ala 10 ruo 15 sekọnd.Na-emikpu warara ahụ ka ọ dịkarịa ala ọkwa nke ahịrị wavy, mana ọ bụghị n'elu akụ na kaadị ule. [Lee foto (1).]

4.Replace okpu ma tinye kaadị ule na-abụghị - absorbent flat elu.

5.Malite ngụ oge wee chere ka ahịrị (s) nwere agba pụta.

6.N'ihi kwesịrị ịgụ na 5 nkeji. Nsonaazụ nwere ike kwụsie ike ruo elekere 1 ka mmalite ule gachara.