【AMFANI DA NUFIN】

Ethyl Glucuronide (ETG) Gwajin gaggawa (Fitsari) shine saurin immunoassay na chromatographic don gano Ethyl Glucuronide a cikin fitsarin ɗan adam. Ethyl Glucuronide da gwajin ya gano ya haɗa da, amma ba'a iyakance shi ba, metabolites na Ethanol.

Wannan kimantawa yana ba da sakamakon gwaji na farko kawai. Dole ne a yi amfani da ƙarin takamaiman hanyar sinadari don samun ingantaccen sakamako na nazari. Gas chromatography/mass spectrometry (GC/MS) shine hanyar tabbatarwa da aka fi so. Ya kamata a yi amfani da la'akari na asibiti da hukuncin ƙwararru ga kowane sakamakon gwajin zagi, musamman lokacin da aka yi amfani da kyakkyawan sakamako na farko.

【TAKAITACCEN】

Ethyl Glucuronide (ETG) wani metabolite ne na barasa na ethyl wanda aka samo shi a cikin jiki ta hanyar glucuronidation bayan fallasa ga ethanol, kamar ta hanyar shan barasa. Ana amfani da shi azaman biomarker don gwada amfani da ethanol kuma don saka idanu kan ƙauracewa barasa a cikin yanayin da aka haramta shan giya, kamar a cikin soja, a cikin shirye-shiryen ƙwararrun likitocin kiwon lafiya. addictions), a cikin makarantu, asibitocin dashen hanta, ko kuma a dawo da masu shan giya.ETG za a iya auna shi a cikin fitsari ta hanyoyin gwaje-gwaje na gargajiya (GC/MS ko LC/MS) har zuwa kimanin sa'o'i 80 bayan an sha ethanol (ya dogara sosai akan adadin giya da abubuwan sha). ETG shine mafi daidaiton alamar bayyanar da barasa fiye da aunawa don kasancewar ethanol kanta.

Gwajin gaggawa na Ethyl Glucuronide (Fitsari) gwajin gwajin fitsari ne mai sauri wanda za'a iya yi ba tare da amfani da kayan aiki ba. Gwajin yana amfani da maganin rigakafi na monoclonal don zaɓin gano girman matakan ethyl glucuronide a cikin fitsarin ɗan adam. Ethyl Glucuronide Rapid Test Strip (Fitsari) yana haifar da sakamako mai kyau lokacin da Ethyl Glucuronide a cikin fitsari ya wuce 500 ng/mL.

【HANYOYIN AMFANI】

Bada gwajin, samfurin fitsari, da/ko sarrafawa don isa zafin ɗaki (15-30ºC) kafin gwaji.

1.Kawo jakar zuwa dakin zafin jiki kafin bude shi. Cire na'urar gwaji daga jakar da aka hatimi kuma amfani da ita cikin sa'a ɗaya.

2. Sanya Na'urar gwaji a kan tsaftataccen wuri mai tsabta. Riƙe digo a tsaye kuma a canja wurin cikakken digo 3 na fitsari (kimanin 120ul) zuwa samfurin da kyau (S) na Na'urar gwaji, sannan fara mai ƙidayar lokaci. Ka guji tarko kumfa a cikin rijiyar samfurin (S). Dubi hoton da ke ƙasa.

3. Jira layin (s) masu launi ya bayyana. Karanta sakamako a minti 5. Kar a fassara sakamakon bayan mintuna 10.

Hanyoyi don Titin Gwaji

4. Kawo jakar zuwa zafin jiki kafin buɗe shi. Cire tsibin gwajin daga jakar da aka hatimi kuma a yi amfani da shi da wuri-wuri.

5. Tare da kibau masu nuni zuwa samfurin fitsari, nutsar da igiyar gwajin a tsaye a cikin samfurin fitsari na aƙalla daƙiƙa 10 zuwa 15.Nitsar da tsiri zuwa aƙalla matakin layukan layukan, amma kar a wuce iyakar iyakar layi (MAX) akan tela gwajin. [Duba hoton (2).]

6. Sanya tsibin gwajin a kan wani wuri mara kyau.

7. Fara mai ƙidayar lokaci kuma jira layin (s) masu launi ya bayyana.

8. Ya kamata a karanta sakamakon a minti 5. Sakamako na iya kasancewa tsayayye har zuwa awa 1 bayan fara gwajin.

Hanyoyi don Katin Gwaji

1. Kawo jakar zuwa zafin jiki kafin buɗe shi. Cire katin gwajin daga jakar da aka rufe kuma yi amfani da shi da wuri-wuri.

2.Cire hula.

3.Tare da kibiya mai nuni zuwa samfurin fitsari, nutsar da katin gwajin a tsaye a cikin samfurin fitsari na akalla daƙiƙa 10 zuwa 15.Nitsar da tsiri zuwa aƙalla matakin layukan layukan, amma ba sama da kibiya akan katin gwajin ba. [Duba hoton (1).]

4.Maye gurbin hular kuma sanya katin gwajin akan wani wuri mara kyau.

5.Fara mai ƙidayar lokaci kuma jira layin (s) masu launi ya bayyana.

6.A sakamakon ya kamata a karanta a 5 minutes. Sakamako na iya kasancewa tsayayye har zuwa awa 1 bayan fara gwajin.