【AMFANI DA NUFIN】

The Cotinine Rapid Test Strip/Katin/Na'ura shine a gefe guda yana kwarara chromatographic immunoassay don gano Cotinine a cikin fitsarin ɗan adam a yanke - 200 ng/mL. Wannan gwajin zai gano sauran mahadi masu alaƙa, da fatan za a koma zuwa Tebur Takaddun Bincike a cikin wannan fakitin.

Wannan kimantawa yana ba da sakamakon gwaji na farko kawai. Dole ne a yi amfani da ƙarin takamaiman hanyar sinadari don samun ingantaccen sakamako na nazari. Gas chromatography da mass spectrometry (GC/MS) shine hanyar tabbatarwa da aka fi so. Ya kamata a yi amfani da la'akari na asibiti da hukuncin ƙwararru ga kowane sakamakon gwajin zagi, musamman lokacin da aka yi amfani da kyakkyawan sakamako na farko.

【TAKAITACCEN】

Cotinine shine farkon - mataki metabolite na nicotine, alkaloid mai guba wanda ke haifar da motsa jiki na ganglia da tsarin juyayi na tsakiya lokacin a cikin mutane.Nicotine magani ne wanda kusan kowane memba na taba Baya ga taba, nicotine kuma ana samunsa ta kasuwanci azaman sinadari mai aiki a cikin hanyoyin maye gurbin shan taba kamar su nicotine danko, facin transdermal da feshin hanci.

A cikin fitsari na awa 24, kusan kashi 5% na adadin nicotine ana fitar dashi azaman magani mara canzawa tare da 10% azaman cotinine da 35% azaman hydroxycotinine; An yi imanin yawan adadin sauran metabolites ya kai kasa da 5% 1 Yayin da ake tunanin cotinine a matsayin metabolite mara aiki, bayanin martabar kawar da shi ya fi karko fiye da na nicotine wanda galibi ya dogara da pH na fitsari.Saboda haka, ana daukar cotinine alama ce mai kyau ta ilimin halitta don tantance amfani da nicotine. Rabin plasma - Rayuwar nicotine kusan mintuna 60 ne bayan shakarwa ko gudanarwar mahaifa.2 Nicotine da cotinine suna saurin kawar da koda; Ana sa ran taga gano cotinine a cikin fitsari a matakin yankewa na 200 ng/mL zai kasance har zuwa kwanaki 2-3 bayan amfani da nicotine.

The Cotinine Rapid Test Strip / Katin / Na'ura shine gwajin gwajin fitsari mai sauri wanda za'a iya yi ba tare da amfani da kayan aiki ba.Gwajin yana amfani da maganin rigakafi na monoclonal don zaɓin gano matakan girma na Cotinine a cikin fitsari.

【HANYOYIN AMFANI】

Bada gwajin, samfurin fitsari, da/ko sarrafawa don isa ga zafin daki (15-30ºC) kafin gwaji.

Don tsiri gwaji

1.Cire Rigon gwajin daga jakar da aka rufe kuma amfani da shi da wuri-wuri.

2. Sanya tsiri a cikin samfurin fitsari tare da ƙarshen kibiya yana nuni zuwa fitsari.

3.Kada a nutsar da layin "Mark." Cire tsiri bayan daƙiƙa 10 sannan a shimfiɗa tsiri, a kwance akan wuri mai tsabta, bushe, maras sha (kamar bakin kwandon fitsari). Jira layin ja ya bayyana..Karanta sakamakon a minti 5 lokacin gwada samfurin.Kada a fassara sakamakon bayan lokacin karantawa da ya dace.Yana da mahimmanci cewa bango ya bayyana a sarari kafin a karanta sakamakon.

Domin Katin Gwaji

1. Daidaita katin gwaji, ko ɗigon gwaji, samfuran fitsari ko sarrafa waje zuwa zafin jiki (15-30°C) kafin gwaji.

2. Cire katin gwajin daga jakar da aka rufe da tsoma katin a cikin samfurin na akalla daƙiƙa 15 zuwa 20 ko kuma har sai ƙaura ta auku. kar a tsoma katin sama da saman layi.

3. Sanya katin gwaji ko ɗigon gwajin akan busasshiyar wuri mai faɗi.

4. Karanta sakamakon a minti 5 zuwa 10.

Don na'urar gwaji

1.Kawo jakar zuwa zafin jiki kafin buɗe shi.Cire na'urar gwaji daga jakar da aka rufe kuma amfani da shi da wuri-wuri.

2. Sanya na'urar gwajin a kan tsaftataccen wuri mai tsabta. Rike digo a tsaye kuma canja wurin 3 cikakkun digo na fitsari (kimanin.10ul) zuwa samfurin rijiyar (S) na na'urar gwajin, sa'an nan kuma fara timer. Ka guji kama kumfa mai iska a cikin samfurin da kyau (S) . Dubi hoton da ke ƙasa.

3. Jira layin (s) masu launi don bayyana. Ya kamata a karanta sakamakon a minti 5. Kada ku fassara sakamakon bayan minti 10.