【ZOYENERA KUGWIRITSA NTCHITO】

Cotinine Rapid Test Strip/Card/Device ndi lateral flow chromatographic immunoassay yozindikiritsa Cotinine mumkodzo wa munthu pakadutsa 200 ng/mL. Mayesowa azindikira mankhwala ena ogwirizana nawo, chonde onani tebulo la Analytical Specific mu phukusili.

Kuyesa uku kumangopereka zotsatira zoyambirira zowunikira. Njira ina yodziwika bwino yamankhwala iyenera kugwiritsidwa ntchito kuti mupeze zotsatira zotsimikizika. Gas chromatography ndi mass spectrometry (GC/MS) ndiyo njira yotsimikizirika yomwe imakonda. Kulingalira zachipatala ndi kuweruza kwa akatswiri kuyenera kugwiritsidwa ntchito pazotsatira zilizonse zoyeserera zakugwiritsa ntchito molakwa mankhwala, makamaka ngati zotsatira zoyambilira zikugwiritsidwa ntchito.

【CHIDULE】

Cotinine ndiye metabolite yoyamba ya chikonga, alkaloid yapoizoni yomwe imatulutsa kukondoweza kwa ganglia ya autonomic ndi dongosolo lapakati la minyewa mukakhala mwa anthu.Chikonga ndi mankhwala omwe pafupifupi membala aliyense wa fodya-osuta amawululidwa kaya mwa kukhudzana mwachindunji kapena kukomoka - Kuphatikiza pa fodya, chikonga chimapezekanso m’malonda ngati mankhwala ochiritsira m’malo osuta monga chikonga, zigamba za transdermal ndi zopopera za m’mphuno.

Mu mkodzo wa maola 24, pafupifupi 5% ya mlingo wa nikotini umatulutsidwa ngati mankhwala osasinthika ndi 10% monga cotinine ndi 35% monga hydroxycotinine; kuchuluka kwa ma metabolites ena kumakhulupirira kuti kumakhala kochepera 5%.1 Ngakhale kuti cotinine imaganiziridwa kuti ndi metabolite yosagwira ntchito, mawonekedwe ake ochotserako amakhala okhazikika kuposa chikonga chomwe makamaka chimadalira pH ya mkodzo. Theka la plasma-moyo wa chikonga umakhala pafupifupi mphindi 60 pambuyo pokoka mpweya kapena kuwongolera kwa makolo.2 Nikotini ndi cotinine zimachotsedwa mwachangu ndi impso; zenera lodziwikiratu kwa cotinine mu mkodzo pa mlingo wodulidwa wa 200 ng/mL akuyembekezeka kukhala masiku 2-3 chikonga chitatha.

Cotinine Rapid Test Strip/Card/Device ndi kuyesa kofulumira kwa mkodzo komwe kungathe kuchitidwa popanda kugwiritsa ntchito chida. Mayesowa amagwiritsa ntchito anti-monoclonal antibody kuti azindikire mosankha kuchuluka kwa Cotinine mumkodzo.

【MALANGIZO OGWIRITSA NTCHITO】

Lolani kuyesa, chitsanzo cha mkodzo, ndi/kapena zowongolera kuti zifikire kutentha (15-30ºC) musanayesedwe.

Kwa test strip

1.Chotsani Mzere Woyesera muthumba losindikizidwa ndikugwiritseni ntchito posachedwa.

2.Mizirani chojambulacho mumkodzo ndipo mapeto ake aloze kumkodzo.

3.Osamiza kupyola Mzere wa “Mark”. Chotsani mzerewo pakadutsa masekondi khumi ndikuyanika mzerewo, ukhale wosanjikiza pamalo oyera, owuma, osayamwa (monga pakamwa pa chidebe cha mkodzo). Dikirani kuti mzere wofiyira uwonekere..Werengani zotsatira pa mphindi zisanu poyesa chitsanzo.Musatanthauzire zotsatira pambuyo pa nthawi yoyenera yowerenga.Ndikofunika kuti maziko amveke bwino musanawerenge zotsatira zake.

Za Mayeso Card

1. Lumikizani khadi loyesera, kapena mzere woyesera, zitsanzo za mkodzo kapena zowongolera kunja ku kutentha kwachipinda (15-30°C) musanayesedwe.

2. Kuchotsa khadi yoyesera m'thumba lomata ndikuviika khadi mu chitsanzocho kwa masekondi osachepera 15 mpaka masekondi 20 kapena mpaka kusamuka kukuchitika. Mizirani mzere (ma) a khadi loyesera pansi pa mzere wapamwamba wa mzere wa mafunde pazitsulo zoyesera; osaviika khadi pamwamba pa mzere wapamwamba.

3. Ikani khadi loyesera kapena mzere woyesera pamtunda wouma.

4. Werengani zotsatira pa mphindi 5 mpaka 10.

Za chipangizo choyesera

1.Bweretsani thumba ku kutentha kwa chipinda musanatsegule.Chotsani chipangizo choyesera muthumba losindikizidwa ndikuchigwiritsa ntchito mwamsanga.

2.Ikani chipangizo choyesera pamalo oyera ndi okwera.Gwirani chotsitsa molunjika ndi kusamutsa madontho atatu a mkodzo (pafupifupi.10ul) ku chitsanzo chabwino (S) cha chipangizo choyesera, ndiyeno yambani chowerengera.Pewani kutchera thovu la mpweya pachitsanzo chabwino (S) .Onani chithunzichi pansipa.

3.Dikirani kuti mzere wachikuda (mizere) uwoneke.Chotsatiracho chiyenera kuwerengedwa pa mphindi 5. Musatanthauzire zotsatira pambuyo pa mphindi 10.