【UKORESHEJWE】

Ikizamini cya Cotinine yihuta / Ikarita / Igikoresho ni immunoassay ikurikira ya chromatografique yo gutahura kugirango tumenye Cotinine mu nkari z'umuntu uciwe - guhagarika 200 ng / mL. Iki kizamini kizagaragaza ibindi bintu bifitanye isano, nyamuneka reba imbonerahamwe ya Analytical yihariye iri muri iyi paki yinjizamo.

Iyi suzuma itanga gusa ibisubizo byibanze byisesengura. Uburyo bwihariye bwo guhinduranya imiti bugomba gukoreshwa kugirango tubone ibisubizo byemejwe byisesenguye. Gazi ya chromatografiya hamwe na mass spectrometrie (GC / MS) nuburyo bwatoranijwe bwo kwemeza. Kwita ku mavuriro no guca imanza z'umwuga bigomba gukoreshwa ku biyobyabwenge byose byakorewe ibizamini, cyane cyane iyo hakoreshejwe ibisubizo byiza byambere.

【INCAMAKE】

Cotinine niyambere - metabolite ya stage ya nikotine, alkaloide yubumara itanga imbaraga zo gutera imbaraga za ganglia autonomic na sisitemu yo hagati yo hagati mugihe abantu.Nicotine numuti hafi ya buri munyamuryango w itabi - societe itabi agaragara haba muburyo butaziguye cyangwa guhumeka kabiri. Usibye itabi, nikotine iraboneka no mu bucuruzi nk'ibikoresho bigira uruhare runini mu kuvura insimburangingo nka nicotine gum, ibibyimba bya transdermal na spray.

Mu nkari z'amasaha 24 -, hafi 5% ya nikotine isohoka nk'imiti idahindutse hamwe na 10% nka cotinine na 35% nka hydroxycotinine; kwibumbira mu zindi metabolite bizwi ko bitageze kuri 5% .1 Mugihe cotinine ikekwa ko ari metabolite idakora, ni umwirondoro wo kurandura uhagaze neza kuruta icya nikotine usanga ahanini inkari pH iterwa.Nkigisubizo, cotinine ifatwa nkikimenyetso cyiza cyibinyabuzima kugirango hamenyekane ikoreshwa rya nikotine. Igice cya plasma - ubuzima bwa nikotine ni iminota 60 ikurikira guhumeka cyangwa ubuyobozi bwababyeyi.2 Nikotine na cotinine bikurwaho vuba nimpyiko; idirishya ryo gutahura cotinine mu nkari kurwego rwo kugabanya 200 ng / mL biteganijwe ko izagera ku minsi 2 - 3 nyuma yo gukoresha nikotine.

Ikizamini cya Cotinine Rapid Ikarita / Ikarita / Igikoresho ni ikizamini cyihuta cyo gusuzuma inkari gishobora gukorwa udakoresheje igikoresho. Ikizamini gikoresha antibody ya monoclonal kugirango ihitemo neza urugero rwa Cotinine iri mu nkari. Ikizamini cya Cotinine Rapid Ikarita / Ikarita / Igikoresho gitanga umusaruro ushimishije iyo Cotinine mu nkari irenze 200 ng / mL.

【AMABWIRIZA YO GUKORESHA】

Emera ikizamini, urugero rwinkari, na / cyangwa kugenzura kugera kubushyuhe bwicyumba (15 - 30ºC) mbere yo kwipimisha.

Ikizamini

1.Kuraho Strip yikizamini mumufuka ufunze kandi uyikoreshe vuba bishoboka.

2. Shira umurongo muri sample yinkari hamwe numwambi wanyuma werekeza inkari.

3.Ntukibire kumurongo wa "Mark". Kuramo umurongo nyuma yamasegonda 10 hanyuma urambike umurongo, urambaraye hejuru yubusa, bwumye, butarimo -.Soma ibisubizo muminota 5 mugihe cyo kugerageza urugero. Ntugasobanure ibisubizo nyuma yigihe gikwiye cyo gusoma.Ni ngombwa ko amateka asobanutse mbere yuko ibisubizo bisomwa.

Ikarita y'Ikizamini

1. Kuringaniza ikarita yikizamini, cyangwa umurongo wikizamini, urugero rwinkari cyangwa kugenzura hanze kubushyuhe bwicyumba (15 - 30 ° C) mbere yo kwipimisha.

. ntukibike ikarita hejuru yumurongo wo hejuru.

3. Shira ikarita yikizamini cyangwa umurongo wikizamini hejuru yumye.

4. Soma ibisubizo kuminota 5 kugeza 10.

Igikoresho cyo kugerageza

1.Kuzana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho igikoresho cyipimisha mumufuka ufunze hanyuma ukoreshe vuba bishoboka.

2. Shyira igikoresho cyo kwipimisha hejuru yisuku kandi iringaniye. Fata igitonyanga uhagarike kandi wohereze ibitonyanga 3 byuzuye byinkari (hafi.10ul) kurugero rwiza (S) rwibikoresho byipimisha, hanyuma utangire igihe. Irinde gufata impemu zumwuka mubyitegererezo neza (S) .Reba ishusho hepfo.

3.Tegereza umurongo (amabara) ugaragara. Ibisubizo bigomba gusomwa muminota 5. Ntugasobanure ibisubizo nyuma yiminota 10.