【UKUSETYENZISWA OKUHLOSIWEYO】

I-Cotinine Rapid Test Strip/Ikhadi/Isixhobo yi-lateral flow chromatographic immunoassay yokukhangela i-Cotinine kumchamo womntu kwi-cut-off concentration ye-200 ng/mL. Olu vavanyo luza kubona ezinye iikhompawundi eziyeleleneyo, nceda ubhekisele kwitheyibhile ye-Analytical Specificity kolu fakelo lwephakheji.

Olu vavanyo lubonelela kuphela ngesiphumo sovavanyo lokuqala. Kufuneka kusetyenziswe enye indlela yeekhemikhali ezizezinye ngakumbi ukuze kufunyanwe iziphumo zohlalutyo eziqinisekisiweyo. I-chromatography yegesi kunye ne-mass spectrometry (GC / MS) yindlela yokuqinisekisa ekhethiweyo. Uqwalaselo lwezonyango kunye nesigwebo sobuchule kufuneka sisetyenziswe kuso nasiphi na isiphumo sovavanyo lokusetyenziswa kakubi kwechiza, ngakumbi xa kusetyenziswa iziphumo zokuqala eziqinisekileyo.

【USHWANKATHELO】

ICotinine yeyokuqala-inqanaba lemetabolite yenikotini, ialkaloid enetyhefu evelisa uvuselelo lwe-autonomic ganglia kunye ne-central nervous system xa iphakathi kwabantu.Nikotine lichiza apho phantse onke amalungu ecuba-abatshayayo bavezwayo nokuba kukudibana ngqo okanye ngokuphefumla ngesandla okwesibini. Ukongeza kwicuba, inikotini ikwafumaneka ngokurhweba njengesithako esisebenzayo kunyango lokubuyisela ukutshaya njengenicotine gum, iipatches ze-transdermal kunye nezitshizi zempumlo.

Kumchamo weyure ezingama-24, malunga ne-5% yedosi ye-nicotine ikhutshwa njengechiza elingatshintshiyo kunye ne-10% njenge-cotinine kunye ne-35% njenge-hydroxycotinine; Ugxininiso lwezinye i-metabolites lukholelwa ukuba lubalelwa ngaphantsi kwe-5% . I-plasma half-life of nicotine about 60 minutes after inhalation or parenteral administration.2 I-Nicotine kunye ne-cotinine zikhutshwa ngokukhawuleza yizintso; ifestile yokufumanisa i-cotinine kumchamo kwinqanaba lokunqunyulwa kwe-200 ng / mL kulindeleke ukuba ibe yi-2-3 iintsuku emva kokusetyenziswa kwe-nicotine.

I-Cotinine Rapid Test Strip / Ikhadi / Isixhobo luvavanyo olukhawulezayo lokuhlola umchamo olunokuthi lwenziwe ngaphandle kokusetyenziswa kwesixhobo. Uvavanyo lusebenzisa i-antibody ye-monoclonal ukukhetha ngokukhethiweyo amanqanaba aphakamileyo e-Cotinine emchameni. I-Cotinine Rapid Test Strip / Card / Isixhobo sivelisa umphumo omuhle xa i-Cotinine idlula / i-2L0 yomchamo.

【IZIKHOKELO ZOKUSETYENZISWA】

Vumela uvavanyo, umzekelo womchamo, kunye/okanye ulawulo lufikelele kubushushu begumbi (15-30ºC) phambi kovavanyo.

Ngomtya wovavanyo

1.Susa i-Trip yovavanyo kwisingxobo esitywiniweyo kwaye uyisebenzise ngokukhawuleza.

2. Gxila umcu kwisampulu yomchamo kwaye isiphelo sotolo sijongise ngakumchamo.

3.Musa ukuntywilisela kumgca othi “Makisha”. Khupha umcu emva kwemizuzwana eyi-10 uze ubeke umcu, umtyaba kwindawo ecocekileyo, eyomileyo, engafunxangayo (efana nomlomo wesingxobo somchamo). Linda kuvele u(imi)mgca obomvu..Funda isiphumo ngemizuzu emi-5 xa uvavanya isampuli.Sukutolika iziphumo emva kwexesha lokufunda elifanelekileyo.Kubalulekile ukuba imvelaphi icace phambi kokuba isiphumo sifundwe.

Ngekhadi loVavanyo

1. Lungelelanisa ikhadi lovavanyo, okanye umtya wovavanyo, iisampulu zomchamo okanye ulawulo lwangaphandle kwiqondo lobushushu begumbi (15-30°C) phambi kovavanyo.

2. Ukususa ikhadi lovavanyo kwisingxobo esivaliweyo kwaye uyithi nkxu ikhadi kwisampulu ubuncinane imizuzwana eyi-15 ukuya kwimizuzwana engama-20 okanye de kuvele imfuduko. Gxininisa imicu yekhadi lovavanyo nje ngaphantsi komgca ongaphezulu womgca wamaza kumaza ovavanyo; musa ukulithi nkxu ikhadi ngaphezu komgca ophezulu.

3. Beka ikhadi lovavanyo okanye umtya wovavanyo kwindawo eyomileyo ethe tyaba.

4. Funda iziphumo ngemizuzu emi-5 ukuya kweli-10.

Kwisixhobo sovavanyo

1.Zisa isingxobo kwiqondo lobushushu begumbi phambi kokusivula.Susa isixhobo sovavanyo kwisingxobo esitywiniweyo kwaye usisebenzise ngokukhawuleza.

2.Beka isixhobo sovavanyo kwindawo ecocekileyo nekumgangatho.Bamba idropha ngokuthe nkqo kwaye udlulisele amathontsi apheleleyo ama-3 omchamo (approx.10ul) kwisampulu yequla (S) lesixhobo sovavanyo, kwaye emva koko uqalise isibali-xesha.Kuphephe ukubambisa amaqamza omoya kumzekelo kakuhle (S) .Jonga lo mfanekiso ungezantsi.

3.Lindela umgca onemibala ukuba uvele.Isiphumo kufuneka sifundwe ngemizuzu emi-5.Musa ukutolika umphumo emva kwemizuzu eyi-10.