【UKUSETSHENZISWA OKUHLOSIWE】

I-Cotinine Rapid Test Strip/Ikhadi/Idivayisi iyi-lateral flow chromatographic immunoassay ukuze kutholwe i-Cotinine emchameni womuntu ekuhlanganiseni okusikiwe okungu-200 ng/mL.Lokhu kuhlolwa kuzothola ezinye izinhlanganisela ezihlobene, sicela ubhekisele kuthebula le-Analytical Specific kulokhu kufakwa kwephakheji.

Lesi sivivinyo sinikeza umphumela wokuqala wokuhlaziya kuphela. Enye indlela yamakhemikhali ecacile kufanele isetshenziswe ukuze kutholwe umphumela wokuhlaziya oqinisekisiwe. I-gas chromatography kanye ne-mass spectrometry (GC/MS) iyindlela yokuqinisekisa ekhethwayo. Ukucatshangelwa komtholampilo kanye nokwahlulela kochwepheshe kufanele kusetshenziswe kunoma yimuphi umphumela wokuhlolwa kokuhlukumeza, ikakhulukazi uma kusetshenziswa imiphumela yokuqala emihle.

【ISIFINYEZO】

I-Cotinine iyi-metabolite yokuqala-yesiteji se-nicotine, i-alkaloid enobuthi ekhiqiza ukugqugquzelwa kwe-autonomic ganglia kanye nesistimu yezinzwa emaphakathi uma ingabantu.I-Nicotine iyisidakamizwa lapho cishe wonke amalungu omphakathi kagwayi - Ngaphezu kukagwayi, i-nicotine iphinde itholakale nokudayiswa njengesithako esisebenzayo ekwelapheni esikhundleni sokubhema njenge-nicotine gum, ama-transdermal patches kanye nezifutho zamakhala.

Ngomchamo wamahora angama-24, cishe u-5% wedosi ye-nicotine ukhishwa njengesidakamizwa esingashintshiwe ngo-10% njenge-cotinine kanye nama-35% njenge-hydroxycotinine; ukugxiliswa kwamanye ama-metabolite kukholakala ukuthi akhawunti ngaphansi kuka-5%.1 Nakuba i-cotinine kucatshangwa ukuthi iyi-metabolite engasebenzi, iphrofayili yayo yokuqeda izinze kakhulu kunaleyo ye-nicotine encike kakhulu kumchamo we-pH.Ngenxa yalokho, i-cotinine ithathwa njengophawu oluhle lwebhayoloji lokunquma ukusetshenziswa kwe-nicotine. Ingxenye ye-plasma-impilo ye-nicotine cishe imizuzu engama-60 ngemva kokuhogela umoya noma ukuphathwa komzali.2 I-Nicotine ne-cotinine zikhishwa ngokushesha yinso; iwindi lokutholwa kwe-cotinine emchameni ezingeni lokunqamuka lika-200 ng/mL kulindeleke ukuthi libe kufika ezinsukwini ezi-2-3 ngemva kokusetshenziswa kwe-nicotine.

I-Cotinine Rapid Test Strip/Ikhadi/Idivayisi ukuhlolwa komchamo okusheshayo okungenziwa ngaphandle kokusebenzisa insimbi. Ukuhlola kusebenzisa i-antibody e-monoclonal ukuthola ngokukhetha amazinga aphakeme e-Cotinine emchamweni.I-Cotinine Rapid Test Strip/Ikhadi/Idivayisi ikhiqiza umphumela omuhle lapho umchamo we-Cotinine/u-2L weqa ku-2L0.

【IZINDLELA ZOKUSEBENZISA】

Vumela ukuhlolwa, isifanekiso somchamo, kanye/noma izilawuli ukuthi zifinyelele izinga lokushisa legumbi (15-30ºC) ngaphambi kokuhlolwa.

Okwe-test strip

1.Khipha I-Strip yokuhlola esikhwameni esivaliwe futhi uyisebenzise ngokushesha ngangokunokwenzeka.

2. Gxilisa umugqa kusampula yomchamo bese ekugcineni komcibisholo ubheke emchameni.

3.Ungacwilisi udlule Emugqeni othi “Maka”. Khipha umucu ngemva kwemizuzwana eyi-10 bese wendlalela umugqa, endaweni ehlanzekile, eyomile, engamunceki (njengomlomo wesiqukathi somchamo). Linda kuvele u(ama)layini obomvu..Funda umphumela ngemizuzu emi-5 lapho uhlola isifanekiso.Ungatoliki imiphumela ngemva kwesikhathi esifanele sokufunda.Kubalulekile ukuthi ingemuva licace ngaphambi kokuba umphumela ufundwe.

Okwekhadi Lokuhlola

1. Linganisa ikhadi lokuhlola, noma umugqa wokuhlola, izibonelo zomchamo noma izilawuli zangaphandle nezinga lokushisa legumbi(15-30°C) ngaphambi kokuhlolwa.

2. Ukukhipha ikhadi lokuhlola esikhwameni esivaliwe bese ucwilisa ikhadi esibonelweni okungenani imizuzwana eyi-15 ukuya kwengama-20 noma kuze kube yilapho kuvela ukufuduka. Gxilisa imicu yekhadi lokuhlola ngezansi nje komugqa ongaphezulu womugqa wamagagasi emicu yokuhlola; ungacwilisi ikhadi ngaphezu komugqa ongaphezulu.

3. Beka ikhadi lokuhlola noma umugqa wokuhlola endaweni eyomile eyisicaba.

4. Funda imiphumela ngemizuzu emi-5 ukuya kweyi-10.

Okwedivayisi yokuhlola

1.Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula.Susa idivayisi yokuhlola esikhwameni esivaliwe futhi usisebenzise ngokushesha ngangokunokwenzeka.

2.Beka idivayisi yokuhlola endaweni ehlanzekile neleveli.Bamba i-dropper ngokuqondile bese udlulisela amaconsi agcwele angu-3 omchamo (cishe.10ul) esibonelweni somthombo(S) wedivayisi yokuhlola, bese uqala isibali sikhathi.Gwema ukucupha amabhamuza omoya esibonelweni kahle (S).Bheka umfanekiso ongezansi.

3.Linda ukuthi kuvele ulayini onemibala.Umphumela kufanele ufundwe ngemizuzu emi-5.Ungahumushi umphumela ngemuva kwemizuzu eyi-10.