【AMFANI DA NUFIN】

Na'urar Gwajin gaggawa ta CK-MB (Dukkan Jini/Serum/Plasma) shine saurin immunoassay na chromatographic don gano ƙimar CKMB na ɗan adam a cikin jini gabaɗayan, magani ko plasma a matsayin taimako a cikin gano cututtukan zuciya na zuciya (MI).

【TAKAITACCEN】

Myoglobin (MYO), Creatine Kinase MB (CK-MB) da cardiac Troponin I (cTnI) sune sunadaran da aka saki a cikin jini bayan rauni na zuciya. Myoglobin shine heme - sunadaran da aka saba samu a cikin kwarangwal da tsoka na zuciya tare da nauyin kwayoyin halitta na 17.8 kDa. Ya ƙunshi kimanin kashi 2 cikin dari na jimlar furotin tsoka kuma yana da alhakin jigilar oxygen a cikin ƙwayoyin tsoka. Matsayin Myoglobin yana ƙaruwa sama da tushe a cikin awanni 2 "B", wanda ya haɗu don samar da isoenzymes daban-daban guda uku, CK-MM, CK-BB da CKMB. CKMB shine isoenzyme na Creatine Kinase wanda ya fi shiga cikin metabolism na tsokar tsoka na zuciya.5 Ana iya gano CK-MB a cikin jini bayan MI a cikin 3-8 hours bayan bayyanar cututtuka. Yana girma a cikin sa'o'i 9 zuwa 30, kuma ya dawo zuwa matakan asali a cikin 48 zuwa 72 hours.6 Cardiac Troponin I shine furotin da aka samo a cikin tsokar zuciya, tare da nauyin kwayoyin halitta na 22.5 kDa.7 Troponin I wani ɓangare ne na rukunin subunit guda uku wanda ya ƙunshi Troponin T da Troponin C. Tare da wannan hadaddun tsarin tropom Ayyukan ATPase na actomyosin a cikin skeletal da tsoka na zuciya.8 Bayan raunin zuciya ya faru, Troponin I an saki a cikin jini 4-6 hours bayan fara jin zafi. Tsarin sakin Troponin I yana kama da CK-MB, amma yayin da matakan CK-MB suka dawo daidai bayan sa'o'i 72, Troponin I ya kasance mai girma har tsawon kwanaki 6-10, don haka yana samar da taga mai tsawo don gano ciwon zuciya.

CK Matsakaicin matakin ganowa shine 5 ng/mL CK-MB.

【HANYAR AMFANI】

Bada gwajin, samfuri da/ko sarrafawa don isa ga zafin jiki (1530°C) kafin gwaji.

1. Kawo jakar a cikin zafin jiki kafin buɗe shi. Cire na'urar gwaji daga jakar da aka rufe kuma amfani da shi da wuri-wuri.Za a sami sakamako mafi kyau idan an yi gwajin nan da nan bayan bude jakar takarda.

2. Sanya na'urar gwajin akan wuri mai tsabta da matakin.

DominSamfuran jini ko Plasma:

Rike digo a tsaye kumacanja wurin digo 1 na jini ko plasma (kimanin 25µl), sannanƙara digo 2 na buffer (kimanin 80 µL) kuma fara mai ƙidayar lokaci.Duba hoton da ke ƙasa.

DominSamfuran Jini Gabaɗayan Venipuncture:

Rike digo a tsaye kumacanja wurin digo 2 na venipuncture duka jini (kimanin 50 µL) zuwa samfurin rijiyar (S) na na'urar gwajin, sannanƙara digo 2 na buffer (kimanin 80 µL) kuma fara mai ƙidayar lokaci.Duba hoton da ke ƙasa.

DominSamfuran Jini na Yatsa:

Izinin 2 digo na rataye na ɗan yatsa gabaɗayan samfurin jini (kimanin 50 µL) don faɗa cikin tsakiyar samfurin da kyau (S) akan na'urar gwaji, sannanƙara digo 2 na buffer (kimanin 80 µL) kuma fara mai ƙidayar lokaci. Dubi hoton da ke ƙasa.

3. Jira layin (s) masu launi ya bayyana.Karanta sakamako a minti 10.Kar a fassara sakamakon bayan mintuna 20.