【TŠEBELETSO TŠEBELETSO】

The CK-MB Rapid Test Device (Whole Blood/Serum/Plasma) ke chromatographic immunoassay e potlakileng bakeng sa ho lemoha boleng ba CKMB ea motho maling a felletseng, serum kapa plasma e le thuso ho hlahlobisiseng lefu la myocardial infarction (MI).

【KAKARETSO】

Myoglobin (MYO),Creatine Kinase MB (CK-MB) le cardiac Troponin I (cTnI) ke liprotheine tse lokolloang maling ka mor'a kotsi ea pelo. Myoglobin ke heme-protheine e atisang ho fumanoa mesifa ea skeletal le ea pelo e nang le boima ba molek'hule ea 17.8 kDa. E etsa karolo ea 2 lekholong ea protheine ea mesifa eohle 'me e ikarabella ho tsamaisa oksijene ka har'a lisele tsa mesifa.1 Ha lisele tsa mesifa li senyehile, Myoglobin e lokolloa maling ka potlako ka lebaka la boholo ba eona bo fokolang. Boemo ba Myoglobin bo eketseha ka mokhoa o lekanyelitsoeng ka holimo ho tekanyo ea pele nakong ea 2-4 hours post-infarct, uping at 9-12 hours, and return to the baseline within 24-36 hours.2,3 CK-MB ke enzyme hape e teng ka har'a mesifa ea pelo, e nang le boima ba molek'hule ea 87.0 kDa.4 Creatine subunits e entsoeng ka "creatine subunits" e entsoeng ka "diskline" e entsoeng ka "diskline" e entsoeng ka diminerale e le "disk" "B", e kopanang ho etsa li-isoenzyme tse tharo tse fapaneng, CK-MM, CK-BB le CKMB. CKMB ke isoenzyme ea Creatine Kinase e amehang haholo ho metabolism ea mesifa ea pelo.5 Ho lokolloa ha CK-MB maling ka mor'a MI ho ka fumanoa ka hare ho 3-8 lihora tse ka mor'a ho qala ha matšoao. E fihla tlhōrōng ka hare ho 9 ho lihora tsa 30, 'me e khutlela maemong a mantlha ka hare ho 48 ho lihora tsa 72. 6 Cardiac Troponin I ke protheine e fumanoang mesifa ea pelo, e nang le boima ba molek'hule ea 22.5 kDa.7 Troponin I ke karolo ea lihlopha tse tharo tse rarahaneng tse nang le Troponin T le Troponin C. Hammoho le motsoako oa motsoako oa troponin, hammoho le motsoako oa motsoako oa troponin o kopanyang motsoako o ka sehloohong. khalsiamo sensitive ATPase mosebetsi oa actomyosin ka striated skeletal le mesifa ea pelo.8 Ka mor'a hore kotsi ea pelo e hlahe, Troponin I e lokolloa maling 4-6 lihora tse ka mor'a ho qala ha bohloko. Mokhoa oa ho lokolloa oa Troponin I o tšoana le CK-MB, empa ha maemo a CK-MB a khutlela ho tloaelehileng ka mor'a lihora tsa 72, Troponin I e lula e phahame bakeng sa 6 - matsatsi a 10, kahoo e fana ka fensetere e telele ea ho lemoha kotsi ea pelo.

The CK-MB Rapid Test Device (Whole Blood/Serum/Plasma) e sebelisa motsoako oa li-antibody tse koahetsoeng le li-reagents ho lemoha ka mokhoa o nepahetseng Myoglobin, CK-MB le Troponin I maling a feletseng, serum kapa plasma. Boemo bo tlase ba ho lemoha ke 5 ng/mL CK-MB.

【TAELO MOSEBETSI】

Dumella teko, mohlala le/kapa ditaolo ho fihlela mocheso wa kamore (1530°C) pele ho teko.

1. Tlisa mokotla mochesong oa kamore pele u o bula.Tlosa sesebelisoa sa teko ka mokotleng o tiisitsoeng 'me u se sebelise kapele kamoo ho ka khonehang.Liphetho tse ntle ka ho fetisisa li tla fumanoa haeba tlhahlobo e etsoa hang ka mor'a ho bula mokotla oa foil.

2. Beha sesebelisoa sa teko sebakeng se hloekileng le se lekaneng.

Bakeng saMehlala ea Serum kapa Plasma:

Tšoara dropper ka holimo lefetisetsa marotholi a 1 a serum kapa plasma (hoo e ka bang 25 μL), joaleeketsa marotholi a 2 a buffer (hoo e ka bang 80 μL) le ho qala selekanyi.Sheba setšoantšo se ka tlase.

Bakeng saMehlala ea Venipuncture Whole Blood:

Tšoara dropper ka holimo lefetisetsa marotholi a 2 a mali a venipuncture (hoo e ka bang 50 µL) ho mohlala oa seliba (S) sa sesebelisoa sa teko, ebeeketsa marotholi a 2 a buffer (hoo e ka bang 80 μL) le ho qala selekanyi.Sheba setšoantšo se ka tlase.

Bakeng saMehlala ea Fingerstick Whole Blood:

Lumella Marotholi a 2 a leketlileng a menoana ea mali (hoo e ka bang 50 µL) ho oela bohareng ba seliba sa mohlala (S) sesebelisoa sa tlhahlobo, ebeeketsa marotholi a 2 a buffer (hoo e ka bang 80 μL) ebe o qala sebali sa nako. Sheba setšoantšo se ka tlase.

3. Emela hore mela e mebala e hlahe.Bala liphetho ka metsotso e 10.Se ke oa toloka liphetho kamora metsotso e 20.