【UKUSETYENZISWA OKUHLOSIWEYO】

Isixhobo se-CK-MB esiKhawulezayo soVavanyo (iGazi elipheleleyo/iSerum/i-Plasma) luvavanyo olukhawulezayo lwe-chromatographic immunoassay yokufumanisa umgangatho we-CKMB yomntu kwigazi elipheleleyo, i-serum okanye i-plasma njengoncedo ekuxilongweni kwe-myocardial infarction (MI).

【USHWANKATHELO】

I-Myoglobin (MYO), i-Creatine Kinase MB (CK-MB) kunye ne-cardiac Troponin I (cTnI) ziiprotheni ezikhutshwe kwigazi emva kokulimala kwentliziyo. I-Myoglobin yi-heme-iprotheyini ngokuqhelekileyo efumaneka kwi-skeletal kunye ne-cardiac muscle kunye ne-molecular weight ye-17.8 kDa.Ibandakanya malunga neepesenti ze-2 zeprotheni ye-muscle epheleleyo kwaye inoxanduva lokuthutha i-oksijini ngaphakathi kweeseli ze-muscle.1 Xa iiseli ze-muscle zonakaliswe, i-Myoglobin ikhutshwa egazini ngokukhawuleza ngenxa yobukhulu bayo obuncinane. Inqanaba le-Myoglobin linyuka ngokulinganayo ngaphezu kwesiseko ngaphakathi kwe-2 - 4 iiyure post-infarct, ukuphakama kwi-9 - iiyure ze-12, kunye nokubuyela kwisiseko phakathi kwe-24-36 iiyure.2,3 CK-MB yi-enzyme ekwakhona kwi-cardiac muscle, ene-molecular weight ye-87.0 kDa.4 I-Creatine ye-subunits ye-disk kunye noyilo lwe-Creatine i-subunits eyi-dizayini evela kwi-disk ephantsi ye-disk kunye ne-dizayini edibeneyo ye-disk. "B", edibanisa ukwenza i-isoenzymes ezintathu ezahlukeneyo, CK - MM, CK - BB kunye ne-CKMB. I-CKMB yi-isoenzyme ye-Creatine Kinase ebandakanyeka kakhulu kwi-metabolism yezicubu ze-cardiac muscle.5 Ukukhululwa kwe-CK - MB kwigazi elilandela i-MI kunokubonwa kwiiyure ze-3 - 8 emva kokuqala kweempawu. Iphezulu kwiiyure ze-9 ukuya kwii-30 iiyure, kwaye ibuyela kumanqanaba asisiseko ngaphakathi kwe-48 kwiiyure ze-72. I-Cardiac Troponin I yiprotheni efumaneka kwi-cardiac muscle, enesisindo se-molecular ye-22.5 kDa.7 i-Troponin I yinxalenye ye-subunit complex equlethwe yi-Troponin T kunye ne-Troponin C. Kanye kunye ne-complex ye-complex complectum kunye ne-complex complectionary complectionary complection Umsebenzi we-ATPase we-calcium we-actomyosin kwi-striated skeletal and cardiac muscle.8 Emva kokulimala kwentliziyo kwenzeka, i-Troponin I ikhutshwa egazini 4-6 iiyure emva kokuqala kweentlungu. Ipatheni yokukhululwa ye-Troponin I ifana ne-CK - MB, kodwa ngelixa i-CK - amanqanaba e-MB abuyela ngokuqhelekileyo emva kweeyure ze-72, i-Troponin I ihlala iphakanyisiwe kwi-6 - iintsuku ze-10, ngaloo ndlela ibonelela ngefestile ende yokufumanisa ukulimala kwenhliziyo.

Isixhobo se-CK-MB esiKhawulezayo soVavanyo (iGazi Elipheleleyo/iSerum/iPlasma) sisebenzisa indibaniselwano yamasuntswana e-antibody egqunywe nge-antibody kwaye ibambe ii-reagents ukuqaphela ngokomgangatho iMyoglobin, CK-MB kunye neTroponin I egazini, kwiserum okanye kwiplasma. Ubuncinci bomgangatho wokufumanisa yi-5 ng/mL CK-MB.

【ISIKHOKELO SOKUSEBENZISA】

Vumela uvavanyo, umzekelo kunye / okanye ulawulo ukufikelela kwiqondo lokushisa (1530 ° C) phambi kovavanyo.

1. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokusivula. Susa isixhobo sovavanyo kwisingxobo esitywiniweyo kwaye usisebenzise ngokukhawuleza kangangoko kunokwenzeka. Iziphumo ezigqwesileyo ziya kufumaneka ukuba uvavanyo lwenziwa ngokukhawuleza emva kokuvula isingxobo sefoyile.

2. Beka isixhobo sokuvavanya kwindawo ecocekileyo kunye nenqanaba.

KubaIisampulu zeSerum okanye zePlasma:

Bamba idropha ngokuthe nkqo kwayeukudlulisa i-1 amaconsi e-serum okanye i-plasma (malunga nama-25 µL), ngokoyongeza amathontsi ama-2 esikhuseli (malunga nama-80 µL) kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

KubaIVenipuncture yeGazi Elipheleleyo:

Bamba idropha ngokuthe nkqo kwayeukutshintshela amathontsi ama-2 egazi elipheleleyo le-venipuncture (malunga nama-50 µL) kumzekelo wequla (S) wesixhobo sovavanyo, emva kokoyongeza amathontsi ama-2 esikhuseli (malunga nama-80 µL) kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

KubaUmnwe weGazi elipheleleyo:

Vumela Amathontsi ama-2 ajingayo omnwe wegazi elipheleleyo (malunga nama-50 µL) ukuwela embindini wequla lomfanekiso (S) kwisixhobo sovavanyo, ngokoyongeza amathontsi ama-2 esikhuseli (malunga nama-80 µL) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.

3. Lindela u(imi)layini onemibala ukuba ivele.Funda iziphumo ngemizuzu eyi-10.Sukutolika iziphumo emva kwemizuzu engama-20.