【ZOYENERA KUGWIRITSA NTCHITO】

The CK-MB Rapid Test Device (Whole Blood/Serum/Plasma) ndi chromatographic immunoassay yofulumira yozindikira CKMB yamunthu m'magazi athunthu, seramu kapena plasma monga chothandizira pakuzindikira matenda a myocardial infarction (MI).

【CHIDULE】

Myoglobin (MYO), Creatine Kinase MB (CK-MB) ndi cardiac Troponin I (cTnI) ndi mapuloteni omwe amatulutsidwa m'magazi pambuyo povulala pamtima. Myoglobin ndi heme-mapuloteni omwe amapezeka m'mitsempha ya chigoba ndi yamtima ndi kulemera kwa molekyulu ya 17.8 kDa. Amapanga pafupifupi 2 peresenti ya mapuloteni onse a minofu ndipo ali ndi udindo woyendetsa mpweya mkati mwa maselo a minofu.1 Pamene maselo a minofu awonongeka, Myoglobin imatulutsidwa m'magazi mofulumira chifukwa cha kukula kwake kochepa. Mlingo wa Myoglobin ukuwonjezeka moyenerera pamwamba pa chiyambi mkati mwa 2-4 hours post-infarct, kukwera pa 9-maola a 12, ndikubwereranso kumalo oyambirira mkati mwa 24-maola 36.2,3 CK-MB ndi puloteni yomwe imapezekanso mu minofu ya mtima, yokhala ndi molekyulu yolemera 87.0 kDa.4 Creatine subunits yopangidwa ndi dipatimenti yopangidwa ndi dipatimenti ya dipatimenti ya dipatimenti ya dipatimenti ya dipatimenti ya dipatimenti ya dipatimenti ya dipatimenti ya mtima. "B", omwe amaphatikiza kupanga ma isoenzymes atatu, CK-MM, CK-BB ndi CKMB. CKMB ndi isoenzyme ya Creatine Kinase yomwe imakhudzidwa kwambiri ndi kagayidwe ka minofu ya mtima.5 Kutulutsidwa kwa CK-MB m'magazi pambuyo pa MI kumatha kuzindikirika mkati mwa 3-8 maola chiyambi cha zizindikiro. Imafika pamtunda wa 9 mpaka maola a 30, ndipo imabwereranso kumagulu oyambirira mkati mwa 48 mpaka maola 72. 6 Cardiac Troponin I ndi puloteni yomwe imapezeka mu minofu ya mtima, yomwe imakhala ndi molekyulu ya 22.5 kDa.7 Troponin I ndi gawo la subunit subunit complex yomwe ili ndi Troponin T ndi Troponin C. Pamodzi ndi zovuta zomwe zimapanga troponin calcium sensitive ATPase ntchito ya actomyosin mu striated chigoba ndi mtima minofu.8 Pambuyo pa kuvulala kwa mtima kumachitika, Troponin I imatulutsidwa m'magazi 4-6 maola pambuyo poyambira kupweteka. Chitsanzo chomasulidwa cha Troponin I ndi chofanana ndi CK-MB, koma pamene CK-MB miyeso imabwereranso pambuyo pa maola a 72, Troponin I imakhalabe yapamwamba kwa 6 - masiku a 10, motero ndikupereka zenera lalitali lodziwikiratu kuvulala kwa mtima.

CK-MB Rapid Test Device (Whole Blood/Serum/Plasma) imagwiritsa ntchito tinthu tating'ono tomwe timakutidwa ndi ma antibody ndikugwira ma reagents kuti azindikire Myoglobin, CK-MB ndi Troponin I m'magazi athunthu, seramu kapena plasma. Mulingo wocheperako ndi 5 ng/mL CK-MB.

【DIRECTION ZOGWIRITSA NTCHITO】

Lolani kuyesa, zitsanzo ndi/kapena zowongolera kuti zifike kutentha (1530 ° C) musanayesedwe.

1. Bweretsani thumba ku kutentha kwa chipinda musanatsegule.Chotsani chipangizo choyesera m'thumba losindikizidwa ndikuchigwiritsa ntchito mwamsanga.Zotsatira zabwino kwambiri zidzapezedwa ngati kuyesa kuchitidwa mwamsanga mutangotsegula thumba la zojambulazo.

2. Ikani chipangizo choyesera pamalo oyera komanso osakanikirana.

ZaZitsanzo za Seramu kapena Plasma:

Gwirani chotsitsacho molunjika ndikusamutsa 1 madontho a seramu kapena plasma (pafupifupi 25 μL),ndiyeonjezani madontho awiri a buffer (pafupifupi 80 μL) ndi kuyamba chowerengera nthawi.Onani chithunzi pansipa.

ZaVenipuncture Magazi Athunthu:

Gwirani chotsitsacho molunjika ndikusamutsa 2 madontho a venipuncture lonse magazi (pafupifupi 50 µL) ku chitsime cha chitsanzo (S) cha chipangizo choyesera, ndiyeonjezani madontho awiri a buffer (pafupifupi 80 μL) ndi kuyamba chowerengera nthawi.Onani chithunzi pansipa.

ZaZitsanzo za Fingerstick Whole Blood:

Lolani Madontho awiri olendewera a ndodo yamagazi athunthu (pafupifupi 50 µL) kugwera pakati pa chitsime cha chitsanzo (S) pa chipangizo choyesera, ndiyeonjezani madontho awiri a buffer (pafupifupi 80 μL) ndi kuyambitsa chowerengera. Onani chithunzi pansipa.

3. Dikirani kuti mizere yachikuda iwonekere.Werengani zotsatira pa mphindi khumi.Osamasulira zotsatira pakatha mphindi 20.