【Ezubere iji】

Ngwa CK-MB ngwa ngwa ule ngwa ngwa (Ọbara dum / Serum / Plasma) bụ ngwa ngwa chromatographic immunoassay maka nchọpụta qualitative nke mmadụ CKMB n'ọbara dum, serum ma ọ bụ plasma dị ka ihe enyemaka na nchoputa nke myocardial infarction (MI).

【Nchịkọta】

Myoglobin (MYO), Creatine Kinase MB (CK-MB) na obi Troponin I (cTnI) bụ protein ndị a na-ewepụta n'ime ọbara mgbe ọ nwesịrị mmerụ ahụ. Myoglobin bụ heme-protein a na-ahụkarị na skeletal na obi akwara nwere ibu molecular nke 17.8 kDa. Ọ bụ ihe dị ka pasent 2 nke protein muscle zuru ezu ma na-ahụ maka ibufe oxygen n'ime sel anụ ahụ. Ọkwa nke Myoglobin na-abawanye nke ọma n'elu ntọala n'ime 2-4 awa post-infarct, na-arị elu na 9-12 awa, na ịlaghachi na ntọala n'ime 24-36 hours.2,3 CK-MB bụ enzyme na-adịkwa na akwara obi, nke nwere ihe dị arọ nke 87.0 kDa.4 Creatine Kinase bụ ụdị nke a na-emepụta site na "dị ka nke a na-emepụta." "B", nke jikọtara iji mepụta isoenzymes atọ dị iche iche, CK - MM, CK - BB na CKMB. CKMB bụ isoenzyme nke Creatine Kinase nke na-etinye aka na metabolism nke anụ ahụ obi obi.5 Ntọhapụ nke CK-MB n'ime ọbara na-esote MI nwere ike ịhụ n'ime 3-8 awa mgbe mmalite nke mgbaàmà. Ọ na-agbago n'ime awa 9 ruo 30, ma laghachi na ọkwa ntọala n'ime awa 48 ruo 72.6 Cardiac Troponin I bụ protein a na-achọta n'ime akwara obi, na ịdị arọ molecular nke 22.5 kDa.7 Troponin I bụ akụkụ nke atọ subunit complex comprised Troponin T na Troponin C. Tinyere ihe a na-emepụta ihe na-eme ka calcium dị mgbagwoju anya nke na-emepụta tropom ATPase ọrụ nke actomyosin na skeletal skeletal na obi obi.8 Mgbe mmerụ ahụ obi gasịrị, a na-ahapụ Troponin I n'ime ọbara 4-6 awa mgbe mmalite nke mgbu. Ụdị ntọhapụ nke Troponin I yiri CK-MB, ma ebe ọkwa CK-MB na-alaghachi na nkịtị mgbe awa 72 gasịrị, Troponin I na-anọgide na-ebuli elu maka 6-10 ụbọchị, si otú a na-enye ohere ịchọta ogologo oge maka mmerụ obi.

Ngwa CK-MB ngwa ngwa nwale (Ọbara dum/Serum/Plasma) na-eji ngwakọta nke ihe mgbochi mmadụ kpuchie ma weghara reagents iji chọpụta nke ọma Myoglobin, CK-MB na Troponin I n'ọbara dum, serum ma ọ bụ plasma. Ọkwa nchọpụta kacha nta bụ 5ng/mL CK-MB.

【Ntuziaka maka ojiji】

Kwe ka nnwale, ụdị na/ma ọ bụ njikwa rute ọnụ ụlọ okpomọkụ (1530°C) tupu nnwale.

1. Weta obere akpa ahụ n'ime ụlọ okpomọkụ tupu imeghe ya. Wepụ ngwaọrụ nyocha ahụ na obere akpa ahụ mechiri emechi ma jiri ya mee ihe ngwa ngwa o kwere omume. A ga-enweta nsonaazụ kacha mma ma ọ bụrụ na a na-eme ule ahụ ozugbo imeghe akpa foil.

2. Debe ngwaọrụ ule na elu dị ọcha na larịị.

MakaỤdị serum ma ọ bụ Plasma:

Jide dobe ahụ kwụ ọtọ nanyefee 1 tụlee nke serum ma ọ bụ plasma (ihe dị ka 25 µl), mgbe ahụtinye 2 tụlee nke ihe nchekwa (ihe dị ka 80 µL) ma malite ngụ oge. Lee ihe atụ n'okpuru.

MakaỤdị Ọbara Nile Venipuncture:

Jide dobe ahụ kwụ ọtọ nanyefee 2 tụlee nke venipuncture ọbara dum (ihe dị ka 50 µL) gaa na ụdị nke ọma (S) nke ngwaọrụ nnwale, weetinye 2 tụlee nke ihe nchekwa (ihe dị ka 80 µL) ma malite ngụ oge. Lee ihe atụ n'okpuru.

MakaỤdị ọbara dum mkpịsị aka:

kwe Mmụba ọbara 2 nkodoro nke mkpịsị mkpịsị aka (ihe dị ka 50 µL) ịdaba n'etiti nke ọma nke ọma (S) na ngwaọrụ nnwale, mgbe ahụ.tinye 2 tụlee nke ihe nchekwa (ihe dị ka 80 µL) ma malite ngụ oge. Lee ihe atụ n'okpuru.

3. Chere ka ahịrị(s) nwere agba pụta.Gụọ nsonaazụ na nkeji iri.Atụgharịla nsonaazụ mgbe nkeji iri abụọ gachara.