【KA MANAO ANA】

ʻO ka CK-MB Rapid Test Device (Whole Blood/Serum/Plasma) he immunoassay chromatographic wikiwiki no ka ʻike qualitative o CKMB kanaka i ke koko holoʻokoʻa, serum a i ʻole ka plasma ma ke ʻano he kōkua i ka hōʻoia ʻana o ka myocardial infarction (MI).

【HOOLAHA】

ʻO Myoglobin (MYO), Creatine Kinase MB (CK-MB) a me Troponin I (cTnI) puʻuwai he mau protein i hoʻokuʻu ʻia i loko o ke kahe koko ma hope o ka hōʻeha puʻuwai. ʻO ka myoglobin he heme-protein i loaʻa maʻamau i loko o ka iwi iwi a me ka puʻuwai puʻuwai me ke kaumaha molekala o 17.8 kDa. Aia ma kahi o 2 pakeneka o ka nui o ka protein muscle a nona ke kuleana no ka lawe ʻana i ka oxygen i loko o nā ʻiʻo ʻiʻo. Ke piʻi nui nei ka pae o ka Myoglobin ma luna o ka palena i loko o 2 - 4 mau hola ma hope - infarct, e piʻi ana ma 9 - 12 hola, a hoʻi i ka papa kuhikuhi i loko o 24 - 36 hola. 2,3 CK-MB he enzyme i loaʻa pū i loko o ka puʻuwai puʻuwai, me ke kaumaha mole o 87.0 kDa.4 Creatine Kinase i hoʻolālā ʻia ma ke ʻano he ʻelua "M" molekula, i hoʻolālā ʻia ma ke ʻano he ʻelua "M" molekula. hui e hana i ekolu isoenzymes, CK-MM, CK-BB a me CKMB. ʻO CKMB ka isoenzyme o Creatine Kinase i komo nui i loko o ka metabolism o ka ʻiʻo ʻiʻo puʻuwai. Piʻi ia i loko o 9 a 30 mau hola, a hoʻi i ka pae kumu i loko o 48 a 72 hola.6 Cardiac Troponin I he protein i loaʻa i loko o ka ʻiʻo cardiac, me ke kaumaha molekala o 22.5 kDa.7 ʻO Troponin I kahi ʻāpana o kahi ʻekolu subunit complex i hui pū ʻia me Troponin T a me Troponin C. Along with this tropomyosin regulated the complex ʻO ka hana ATPase o actomyosin i loko o ka iwi iwi a me ka puʻuwai puʻuwai.8 Ma hope o ka hōʻeha ʻana o ka naʻau, hoʻokuʻu ʻia ʻo Troponin I i ke koko 4 - 6 mau hola ma hope o ka hoʻomaka ʻana o ka ʻeha. Ua like ka hoʻokuʻu ʻana o Troponin I me CK-MB, akā ʻoiai e hoʻi ka pae CK-MB i ka maʻamau ma hope o nā hola 72, hoʻokiʻekiʻe ʻia ʻo Troponin I no 6-10 mau lā, no laila e hāʻawi ana i kahi pukaaniani lōʻihi o ka ʻike ʻana no ka hōʻeha puʻuwai.

Hoʻohana ka CK-MB Rapid Test Device (Whole Blood/Serum/Plasma) i ka hui pū ʻana o nā ʻāpana antibody coated a hopu i nā reagents e ʻike pono i ka Myoglobin, CK-MB a me Troponin I i ke koko holoʻokoʻa, serum a i ʻole plasma. ʻO ka pae haʻahaʻa haʻahaʻa he 5 ng/mL CK-MB.

【OLELO HOOLAHA】

E ʻae i ka hoʻāʻo, ka hōʻailona a/a i ʻole nā ​​mana e hiki i ka wela lumi (1530°C) ma mua o ka hoʻāʻo ʻana.

1. E lawe mai i ka ʻeke i ka lumi wela ma mua o ka wehe ʻana. Wehe i ka mea hoʻāʻo mai ka ʻeke i hoʻopaʻa ʻia a hoʻohana iā ia i ka hiki.

2. E kau i ka mea ho'āʻo ma kahi maʻemaʻe a pae.

No ka meaNā hōʻailona Serum a i ʻole Plasma:

E hoʻopaʻa i ka dropper me ka pololeihoʻololi i 1 kulu o ka serum a i ʻole ka plasma (ma kahi o 25 µL),a lailahoʻohui i 2 kulu o ka buffer (ma kahi o 80 µL) a hoʻomaka i ka manawa. E nānā i ke kiʻi ma lalo.

No ka meaVenipuncture Whole Blood specimens:

E hoʻopaʻa i ka dropper me ka pololeihoʻoili i 2 kulu o ka venipuncture holoʻokoʻa koko (ma kahi o 50 µL) i ka pūnāwai specimen (S) o ka mea hoʻāʻo, a lailahoʻohui i 2 kulu o ka buffer (ma kahi o 80 µL) a hoʻomaka i ka manawa. E nānā i ke kiʻi ma lalo.

No ka meaʻO nā hiʻohiʻona o ke koko piha lima lima:

ʻAe 2 kulu kaawe o ka manamana lima o ke koko holookoa (ma kahi o 50 µL) e hāʻule i waenakonu o ka pūnāwai specimen (S) ma ka mea hoʻāʻo, a lailahoʻohui i 2 kulu o ka buffer (ma kahi o 80 µL) a hoʻomaka i ka manawa. E nānā i ke kiʻi ma lalo.

3. E kali a puka mai ka laina kala.E heluhelu i nā hualoaʻa ma 10 minuke.Mai wehewehe i nā hopena ma hope o 20 mau minuke.