【Imigaqo yeNkqubo】

IKit ye-Assay ye-Assay ye-2019-nCOV IgG ye-Assay (CMIA) sisibini-inyathelo lokugonyela umzimba kusetyenziswa itekhnoloji yemagnethi ye-chemiluminescence. Inokuqinisekisa ngokuchanekileyo ubukho be-anti-2019-nCoVIgG antibodies kwiserum yomntu okanye kwiplasma.

Ngexesha lokusabela, inyathelo lokuqala kukubopha iprotheyini ngokuxuba isampuli kunye namasuntswana eparamagnetic anamandla agqunywe nge-2019 - nCoV yeprotein ye-antigen. Emva kokuhlamba, i-alkaline phosphataselabeled murine anti-human IgG monoclonal antibody yongezwa kwinyathelo lesibini ukwenza isuntswana magnetic-nucleoprotein_anti-2019-nCoV -IgG_murine-anti-IgG- ALP-enzyme reaction umxube. Emva komnye umjikelezo wokuhlamba, i-substrate ye-alkaline phosphatase yongezwa kumxube wokuphendula. Isiphumo sesiphumo se-chemiluminescence reaction silinganiswa kwiiyunithi zokukhanya ezihambelanayo (RLU). Uxinaniso lwe-anti-2019-nCoV -IgG ye-antibody kwisampulu luthelekelelwa kwisixa seyunithi yokukhanya okuzalanayo (RLU) yomxube. Ke isiphumo sinokusetyenziselwa ukubona ubukho be-IgG kwisampulu kunye noncedo lokufumanisa ukuba isigulana sosulelwe yintsholongwane ka-2019-nCoV.