【Nā Kumu o ke Kaʻina Hana】

ʻO ka 2019-nCOV IgM Antibody Assay Reagent Kit (CMIA) he ʻelua-step immunoassay me ka hoʻohana ʻana i ka ʻenehana chemiluminescence particle magnetic. Hiki iā ia ke hoʻoholo pololei i ka loaʻa ʻana o nā antibodies anti-2019-nCOVIgM i loko o ka serum kanaka a i ʻole ka plasma.

I ka wā o ka pane ʻana, ʻo ka hana mua ka hoʻopaʻa ʻana i ka protein ma ka hui ʻana i ka laʻana me nā ʻāpana paramagnetic ikaika i uhi ʻia me ka 2019-nCoV nuclear protein antigen. Ma hope o ka holoi ʻana, hoʻohui ʻia ka alkaline phosphataselabeled murine anti-human IgM monoclonal antibody i ka lua o ka hana e hana ai i kahi ʻāpana magnetic-nucleoprotein_anti-2019-nCoV-IgM murine-anti-IgM-ALP-enzyme reaction mixture. Ma hope o kekahi pōʻai holoi, hoʻohui ʻia ka substrate o ka alkaline phosphatase i ka hui ʻana. A laila ana ʻia ka hopena chemiluminescence i nā ʻāpana māmā pili (RLU). ʻO ka ʻike ʻana o ka anti-2019-nCoV -IgM antibody i loko o ka laʻana e like me ka nui o nā ʻāpana kukui pili (RLU) o ka hui. No laila hiki ke hoʻohana ʻia ka hopena e ʻike i ke ola ʻana o ka IgM i ka laʻana a kōkua e hoʻoholo inā ua loaʻa ka maʻi i ka maʻi 2019-nCoV.