【Mfundo za Ndondomeko】

The 2019-nCOV IgM Antibody Assay Reagent Kit (CMIA) ndi njira ziwiri zoyesera zodzitetezera pogwiritsa ntchito ukadaulo wa maginito a chemiluminescence. Itha kudziwa bwino kukhalapo kwa ma anti-2019-nCOVIgM ma antibodies mu seramu yamunthu kapena madzi a m'magazi.

Panthawiyi, gawo loyamba ndikumanga mapuloteni posakaniza chitsanzocho ndi tinthu tating'onoting'ono ta paramagnetic tomwe timakutidwa ndi antigen ya nyukiliya ya 2019-nCoV. Pambuyo kutsuka, alkaline phosphataselabeled murine anti-human IgM monoclonal antibody amawonjezedwa mu sitepe yachiwiri kuti apange magnetic particle-nucleoprotein_anti-2019-nCoV-IgM murine-anti-IgM-ALP-enzyme reaction mix. Pambuyo pa kusamba kwina, gawo lapansi la alkaline phosphatase limawonjezeredwa kusakaniza komweko. Zotsatira zake za chemiluminescence zimayesedwa mu mayunitsi a kuwala (RLU). Kuchuluka kwa ma anti-2019-nCoV -IgM antibody mu sampuli ndi molingana ndi kuchuluka kwa mayunitsi opepuka (RLU) a kusakaniza. Chifukwa chake zotsatira zake zitha kugwiritsidwa ntchito kuzindikira kukhalapo kwa IgM pachitsanzocho ndikuthandizira kudziwa ngati wodwalayo watenga kachilombo ka 2019-nCoV.