【ZOFUNIKA KUGWIRITSA NTCHITO】

Cardiac Troponin I Rapid Test Device (Colloidal Gold) ndi njira yoyesera yodziwira matenda a mtima wa munthu Troponin I m'magazi athunthu, seramu kapena madzi a m'magazi monga chothandizira kuzindikira myocardial infarction (MI).

【CHIDULE】

Cardiac Troponin I (cTnI) ndi puloteni yomwe imapezeka mu minofu ya mtima ndi molekyulu yolemera 22.5 kDa.1 Troponin I ndi gawo la magawo atatu a subunit complex omwe ali ndi Troponin T ndi Troponin C. Pamodzi ndi tropomyosin, chigawo ichi chimapanga chigawo chachikulu chomwe chimayang'anira ntchito ya calcium activate sensitivity ya ATPase minofu ya ATPase. 2 Pambuyo pa kuvulala kwa mtima, Troponin I imatulutsidwa m'magazi 4 - maola 6 pambuyo poyambira kupweteka. Njira yotulutsira cTnI ndi yofanana ndi CK-MB, koma pamene CK-MB miyeso imabwereranso pambuyo pa maola a 72, Troponin I imakhalabe pamwamba kwa 6 - masiku a 10, motero ndikupereka zenera lalitali lozindikira kuvulala kwa mtima. Kukhazikika kwapamwamba kwa miyeso ya cTnI yozindikiritsa kuwonongeka kwa myocardial yawonetsedwa muzochitika monga nthawi yochita opaleshoni, pambuyo pa kuthamanga kwa marathon, ndi kupwetekedwa mtima kwa chifuwa. Kutulutsidwa kwa 3 cTnI kwalembedwanso m'mikhalidwe yamtima kupatula acute myocardial infarction (AMI) monga angina wosakhazikika, kulephera kwa mtima, komanso kuwonongeka kwa ischemic chifukwa cha opaleshoni yodutsa mtsempha wamagazi. 4 Chifukwa chapamwamba kwambiri komanso kukhudzidwa kwa minofu ya myocardial, Troponin I posachedwapa wakhala biomarker yokondedwa kwambiri ya myocardial infarction.5

Cardiac Troponin I Rapid Test Device(Colloidal Gold) ndi kuyesa kosavuta komwe kumagwiritsa ntchito tinthu tating'ono ta anti-cTnI antibody totchinga ndi reagent yojambula kuti azindikire cTnI m'magazi athunthu, seramu kapena plasma. Mulingo wocheperako ndi 0.5ng/mL.

【DIRECTION ZOGWIRITSA NTCHITO】

Lolani kuyesa, chitsanzo, buffer ndi/kapena zowongolera kuti zifikire kutentha kwachipinda (15-30℃) asanayesedwe.

1.Bweretsani thumba kutentha kwa chipinda musanatsegule.Chotsani chipangizo choyesera kuchokera m'thumba losindikizidwa ndikuchigwiritsa ntchito mwamsanga.

2.Ikani Chipangizocho pamalo oyera komanso okwera.

ZaSeramu kapena Plasma chitsanzo:

Gwirani chotsitsa molunjika ndikusuntha2 madontho a seramu kapena plasma (pafupifupi 50).µL)kudera lachitsanzo, ndiye onjezani madontho 1 a buffer(pafupifupi 40µL), ndikuyamba chowerengera nthawi. Onani chithunzi pansipa.

ZaVenipuncture Magazi Athunthu:

Gwirani chotsitsa molunjika ndikusunthaMadontho atatu amagazi athunthu (pafupifupi75µL) kupita kudera lachitsanzo, ndiyeonjezani madontho 1 a buffer (pafupifupi40µL),ndipo yambani chowerengera nthawi.Onani chithunzi pansipa.

ZaChitsanzo cha Fingerstick Whole Blood:

Kugwiritsa ntchito chubu cha capillary: Dzazani chubu cha capillaryndi kusamutsa pafupifupi 75µL of chala chathunthu magazikupita kugawo lachitsanzo la Chida choyesera, ndiye onjezani dontho limodzi la buffer (pafupifupi 40µL) ndi kuyamba chowerengera nthawi.Onani chithunzi pansipa.

Kugwiritsa ntchito madontho olendewera: LolaniMadontho atatu olendewera a ndodo yamagazi athunthu (pafupifupi 75µL) kugwera m'gawo lachitsanzo la Chida choyesera, ndiyeonjezani 1 dontho la buffer (pafupifupi 40µL) ndi kuyamba chowerengera nthawi.Onani chithunzi pansipa.

3. Dikirani kuti mizere yachikuda iwonekere.Werengani zotsatira pa mphindi khumi.Osamasulira zotsatira pakatha mphindi 20.