【UKORESHEJWE】

Igikoresho cyo Kwipimisha Cardiac Troponine I (Colloidal Gold) ni immunoassay yihuta ya chromatografique kugirango hamenyekane neza umutima wumutima wa Troponine wa I mumaraso yose, serumu cyangwa plasma nkubufasha mugupima indwara ya myocardial (MI).

【INCAMAKE】

Cardiac Troponin I (cTnI) ni poroteyine iboneka mu mitsi yumutima ifite uburemere bwa molekile ingana na 22.5 kDa.1 Troponin I igizwe na subunit eshatu igizwe na Troponin T na Troponin C. Hamwe na tropomyosine, uru ruganda rwubaka rugizwe ningingo nyamukuru igenga ibikorwa bya calcium yumutima wa actomyosine. 2 Nyuma yo gukomeretsa k'umutima, Troponin I irekurwa mumaraso 4 - amasaha 6 nyuma yububabare butangiye. Uburyo bwo kurekura cTnI busa na CK - MB, ariko mugihe urwego rwa CK - MB rusubira mubisanzwe nyuma yamasaha 72, Troponin I ikomeza kuzamuka muminsi 6 - 10, bityo igatanga idirishya rirerire ryo kumenya ibikomere byumutima. Umwihariko wo gupima cTnI kugirango umenye ibyangiritse bya myocardial byagaragaye mubihe nkigihe cya perioperative, nyuma ya marato yiruka, hamwe nihungabana ryigituza. 3 cTnI irekurwa kandi yanditswe mubihe byumutima usibye infarction ikaze ya myocardial (AMI) nka angina idahindagurika, kunanirwa k'umutima, hamwe no kwangirika kwa ischemic bitewe no kubaga imitsi ya koronari. 4 Kubera umwihariko wacyo hamwe nubukangurambaga mumyanya myocardial, Troponin I mperutse kuba biomarker ikunzwe cyane kuri infirasiyo ya myocardial.5

Cardiac Troponin I Igikoresho Cyipimisha Cyihuta (Zahabu ya Colloidal) nikizamini cyoroshye gikoresha uruvange rwa antibody - Urwego ntarengwa rwo kumenya ni 0.5ng / mL.

【AMABWIRIZA YO GUKORESHA】

Emera ikizamini, ingero, buffer na / cyangwa igenzura kugera kubushyuhe bwicyumba (15 - 30℃) mbere yo kwipimisha.

1.Kuzana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho igikoresho cyikizamini mumufuka ufunze hanyuma ukoreshe vuba bishoboka.

2. Shyira Igikoresho hejuru kandi isukuye.

KuriSerumu cyangwa Plasma urugero:

Fata igitonyanga gihagaritse kandi wimureIbitonyanga 2 bya serumu cyangwa plasma (hafi 50µL)Kuri icyitegererezo, hanyuma ongeramo ibitonyanga 1 bya buffer(hafi 40µL), hanyuma utangire ingengabihe. Reba ingero zikurikira.

KuriVenipuncture Amaraso Yuzuye:

Fata igitonyanga gihagaritse kandi wimureIbitonyanga 3 byamaraso yose (hafi75µL) kumwanya wikigereranyo, hanyumaongeramo ibitonyanga 1 bya buffer (hafi40µL), hanyuma utangire igihe. Reba ikigereranyo hepfo.

KuriUrutoki Amaraso Yuzuye:

Gukoresha umuyoboro wa capillary: Uzuza umuyoboro wa capillaryno kwimura hafi 75µL of urutoki rwamaraso yosekumwanya wikigereranyo cyibizamini Igikoresho, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40µL) hanyuma utangire igihe. Reba ingero hepfo.

Gukoresha ibitonyanga bimanikwa: Emerera3 kumanika ibitonyanga byintoki zamaraso yose (hafi 75µL) kugwa mubice byikigereranyo cyibizamini, hanyumaongeramo igitonyanga 1 cya buffer (hafi 40µL) hanyuma utangire igihe. Reba ingero hepfo.

3. Tegereza umurongo (amabara) ugaragara.Soma ibisubizo muminota 10.Ntugasobanure ibisubizo nyuma yiminota 20.