【Ezubere iji】

Ngwa ule ngwa ngwa PSA (Ọbara dum/Serum/Plasma) bụ ngwa ngwa immunoassay chromatographic maka nchọpụta qualitative nke Prostate Specific Antigen n'ime ọbara dum, serum ma ọ bụ plasma.

【Nchịkọta】

A na-emepụta prostate kpọmkwem antigen (PSA) site na sel prostate glandular na endothelial. Ọ bụ otu yinye glycoprotein nwere nha molekụla nke ihe dịka 34 kDa.1 PSA dị n'ụdị isi atọ na-ekesa na serum. Ụdị ndị a bụ PSA n'efu, PSA jikọtara ya na α1 - Antichymotrypsin (PSA - ACT) na PSA complexed na α2-macroglobulin (PSA - MG) .2 PSA achọpụtala na anụ ahụ dị iche iche nke usoro urogenital nwoke mana ọ bụ naanị prostate glandular na endothelial cell na-ezobe ya. Ọkwa PSA na serum0 na ahụike nwoke dị n'etiti 6. ng/mL.Ọ nwere ike ibuli elu na ajọ ọnọdụ dị ka prostate cancer, na adịghị mma dị ka benign prostatic hyperplasia na prostatitis.A PSA larịị nke 3 ka 10ng/ml a na-ewere dị na "isi awọ-mpaghara" na ọkwa n'elu 10ng/ml bụ nke ukwuu na-egosi nke ọrịa cancer. biopsy.

Nnwale prostate kpọmkwem antigen bụ ngwá ọrụ kasị baa uru maka nchọpụta nke ọrịa cancer prostate n'oge. Ọtụtụ nchọpụta ekwenyela na ọnụnọ PSA bụ ihe nrịbama tumor kachasị bara uru na nke bara uru mara maka ọrịa cancer prostate na ọrịa prostate nke Benign Prostatic Hyperplasia (BPH) .4

Ngwa ule ngwa ngwa PSA (Ọbara dum/Serum/Plasma) na-eji ngwakọta nke colloidal gold conjugate na mgbochi PSA iji họrọ chọpụta mkpokọta PSA n'ọbara dum, serum ma ọ bụ plasma. Nnwale ahụ nwere uru mbelata - 3ng/ml yana uru ntụaka nke 10ng/ml.

【Ntuziaka maka ojiji】

Kwe ka nnwale ahụ, ihe nlere, ihe nchekwa na/ma ọ bụ njikwa ka ọ rute okpomọkụ nke ọnụ ụlọ (15-30°C) tupu nnwale.

1. Weta obere akpa ahụ n'ime ụlọ okpomọkụ tupu imeghe ya. Wepụ ngwaọrụ nyocha na obere akpa ahụ mechiri emechi ma jiri ya mee ihe ozugbo enwere ike. Gbanyụọ taabụ nke vial nchekwa na-enweghị ịpịa.

2. Debe ngwaọrụ ahụ n'elu ala dị ọcha ma dị larịị.

MakaSerum,Ụdị Plasma:

Jide dropper ahụ kwụ ọtọ ma bufee 1 drop nke serum ma ọ bụ plasma (ihe dị ka 40uL) n'ụdị nke ọma (S) nke ngwaọrụ nnwale, wee tinye 1 dobe nchekwa (ihe dị ka 40uL) wee malite ngụ oge.

MakaVenipuncture zuru ọbara ihe nlere:

Jupụta tube capillary na 2 tụlee nke venipuncture ọbara dum (ihe dị ka 80uL) n'ụdị ihe atụ nke ngwaọrụ nnwale, wee tinye 1 dobe nchekwa (ihe dị ka 40uL) wee malite ngụ oge.

MakaNlere ọbara dum mkpịsị aka:

Iji jiri tube capillary: Jujuo tube capillary wee bufee 2 mịrị mkpisiaka mkpịsị aka n'ọbara niile (ihe dị ka 80uL) n'akụkụ ihe nlele ngwaọrụ, wee tinye 1 dobe nchekwa (ihe dị ka 40uL) wee malite ngụ oge.

3. Chere ka ahịrị(s) nwere agba pụta*.Gụọ nsonaazụ na nkeji iri na ise.Atụgharịla nsonaazụ ya mgbe nkeji iri abụọ gachara.