【UKORESHEJWE】

Igikoresho cyihuta cya PSA (Amaraso Yuzuye / Serumu / Plasma) ni immunoassay yihuta ya chromatografique kugirango tumenye neza Antigen yihariye ya Prostate mumaraso yose, serumu cyangwa plasma.

【INCAMAKE】

Antigen yihariye ya prostate (PSA) ikorwa na prostate glandular na selile endothelia. Ni urunigi rumwe glycoproteine ​​ifite uburemere bwa molekuline igera kuri 34 kDa.1 PSA ibaho muburyo butatu buzenguruka muri serumu. Izi fomu ni ubuntu PSA, PSA ihujwe na α1 - Antichymotrypsin (PSA - ACT) na PSA igizwe na α2 - macroglobuline (PSA - MG) .2 PSA yagaragaye mubice bitandukanye bigize sisitemu ya urogenital yumugabo ariko prostate glandular na endoteliyale gusa irabisohora. Urwego rwa PSA muri serumu yabagabo bafite ubuzima bwiza ni hagati ya 0.1ng / mL. kanseri ya prostate, kandi mumeze neza nka hyperplasia benigne prostate na prostatite.Urwego rwa PSA rwa 3 kugeza 10ng / ml rufatwa nkaho ruri muri "gray - zone" kandi urwego ruri hejuru ya 10ng / ml rugaragaza cyane kanseri.3 Abarwayi bafite agaciro ka PSA hagati ya 3 - 10ng / ml bagomba gukomeza gusesengura prostate na biopsy.

Ikizamini cya antigen yihariye ya prostate nigikoresho cyingirakamaro kiboneka mugupima kanseri ya prostate hakiri kare. Ubushakashatsi bwinshi bwemeje ko kuba PSA ari ikimenyetso cyibibyimba byingirakamaro kandi bifite akamaro bizwi cyane kuri kanseri ya prostate no kwandura prostate ya Benign Prostatic Hyperplasia (BPH) .4

Igikoresho cyihuta cya PSA (Amaraso Yuzuye / Serumu / Plasma) ikoresha ikomatanya rya zahabu ya conjugate na anti - PSA kugirango ihitemo neza PSA yuzuye mumaraso yose, serumu cyangwa plasma. Ikizamini gifite igabanuka - agaciro ka 3ng / ml hamwe nagaciro ka 10ng / ml.

【AMABWIRIZA YO GUKORESHA】

Emera ikizamini, icyitegererezo, buffer na / cyangwa igenzura kugera ku bushyuhe bwicyumba (15 - 30 ° C) mbere yo kwipimisha.

1. Zana umufuka mubushyuhe bwicyumba mbere yo kuyifungura. Kuraho igikoresho cyipimisha mumufuka ufunze hanyuma ukoreshe vuba bishoboka. Kuraho tab ya vial viffe utiriwe ukanda.

2. Shira igikoresho hejuru yisuku kandi iringaniye.

KuriSerumu,Urugero rwa plasma:

Fata igitonyanga gihagaritse hanyuma wohereze igitonyanga 1 cya serumu cyangwa plasma (hafi 40uL) kurugero rwiza (S) rwibikoresho byipimisha, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40uL) hanyuma utangire igihe.

KuriAmaraso Yuzuye urugero:

Uzuza umuyoboro wa capillary hamwe nigitonyanga 2 cyamaraso ya venipuncture yamaraso yose (hafi 80uL) mukigereranyo cyibikoresho byapimwe, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40uL) hanyuma utangire igihe.

KuriUrutoki Amaraso Yuzuye:

Kugira ngo ukoreshe umuyoboro wa capillary: Uzuza umuyoboro wa capillary hanyuma wohereze ibitonyanga 2 byintoki zintoki zose zamaraso (hafi 80uL) mukarere kerekana ibikoresho bipimisha, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40uL) hanyuma utangire igihe.

3. Tegereza umurongo wamabara ugaragara *.Soma ibisubizo muminota 15.Ntugasobanure ibisubizo nyuma yiminota 20.