【UKUSETSHENZISWA OKUHLOSIWE】

I-PSA Rapid Test Device (Whole Blood/Serum/Plasma) iwukuhlola okusheshayo kwe-chromatographic ukuze kutholwe ikhwalithi ye-Prostate Specific Antigen egazini lonke, i-serum noma i-plasma.

【ISIFINYEZO】

I-Prostate specific antigen (PSA) ikhiqizwa amaseli e-prostate glandular kanye ne-endothelial. Iketango elilodwa le-glycoprotein elinesisindo sengqamuzana esilinganiselwa ku-34 kDa.1 I-PSA itholakala ngezindlela ezintathu ezinkulu ezijikeleza ku-serum. Lawa mafomu ayi-PSA yamahhala, i-PSA eboshelwe ku-α1 – Antichymotrypsin (PSA-ACT) kanye ne-PSA ehlanganiswe ne-α2–macroglobulin (PSA-MG).2 I-PSA itholwe ezicutshini ezihlukahlukene zesistimu ye-urogenital yesilisa kodwa kuphela i-prostate glandular kanye namaseli endothelial ayikhiphayo.Izinga le-PSA liphakathi kwe-6/m2 ye-serum2. ng/mL.Ingakhushulwa ezimeni eziyingozi njengomdlavuza wendlala yesinye, futhi isesimweni esibucayi njenge-benign prostatic hyperplasia kanye ne-prostatitis.Izinga le-PSA elingu-3 ukuya ku-10ng/ml libhekwa “njengempunga-zone” futhi amazinga angaphezu kuka-10ng/ml abonisa kakhulu umdlavuza.3 Iziguli ezine-prostate ezingaphansi kwe-3 nge-biopsy.

Ukuhlolwa kwe-prostate ethize ye-antigen iyithuluzi elibaluleke kakhulu elitholakalayo lokuxilongwa komdlavuza wendlala yesinye ekuqaleni.Ucwaningo oluningi luye lwaqinisekisa ukuthi ukuba khona kwe-PSA kuwuphawu lwesimila oluwusizo kakhulu futhi olunenjongo elaziwa ngomdlavuza wendlala yesinye kanye ne-prostate infection ye-Benign Prostatic Hyperplasia (BPH).4

I-PSA Rapid Test Device (Igazi Eliphelele/I-Serum/Plasma) isebenzisa inhlanganisela ye-colloidal gold conjugate kanye nama-anti-PSA amasosha omzimba ukuze athole ngokukhetha isamba se-PSA egazini lonke, i-serum noma i-plasma. Ukuhlola kunenani elisikiwe elingu-3ng/ml kanye nenani eliyireferensi elingu-10ng/ml.

【IZINDLELA ZOKUSEBENZISA】

Vumela ukuhlola, isifanekiso, ibhafa kanye/noma izilawuli ukuthi zifinyelele izinga lokushisa legumbi (15-30°C) ngaphambi kokuhlolwa.

1. Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula.Khipha idivayisi yokuhlola esikhwameni esivaliwe futhi uyisebenzise ngokushesha ngangokunokwenzeka.Sula ithebhu ye-vial yebhafa ngaphandle kokuyikhama.

2. Beka idivayisi endaweni ehlanzekile nesezingeni.

NgobaI-Serum,Izibonelo zePlasma:

Bamba i-dropper ngokuqondile bese udlulisela iconsi elingu-1 le-serum noma i-plasma (cishe u-40uL) esibonelweni somthombo (S) wedivayisi yokuhlola, bese wengeza ithonsi le-buffer elingu-1 (cishe u-40uL) bese uqala isibali sikhathi.

NgobaI-Venipuncture Igazi Eliphelele isibonelo:

Gcwalisa ishubhu le-capillary kanye namaconsi angu-2 egazi eliphelele le-venipuncture (cishe ama-80uL) endaweni yesampula yedivayisi yokuhlola, bese wengeza ithonsi le-buffer elingu-1 (cishe u-40uL) bese uqala isibali sikhathi.

NgobaIsifanekiso seFingerstick Whole Blood:

Ukuze usebenzise ishubhu le-capillary:Gcwalisa ishubhu le-capillary futhi udlulisele amaconsi angu-2 esampula yegazi lomunwe (cishe u-80uL) endaweni yesifanekiso sedivayisi yokuhlola, bese ungeza iconsi elingu-1 lebhafa (cishe u-40uL) bese uqala isibali sikhathi.

3. Linda kuze kuvele i(ama)layini enombala*.Funda imiphumela ngemizuzu eyi-15.Ungahumushi umphumela ngemuva kwemizuzu engama-20.