【UKUSETYENZISWA OKUHLOSIWEYO】

I-PSA Rapid Test Device (Igazi Elilonke/iSerum/Plasma) luvavanyo olukhawulezayo lwe-chromatographic yokubona umgangatho we-Prostate Specific Antigen kwigazi lonke, serum okanye kwiplasma.

【USHWANKATHELO】

I-Prostate specific antigen (PSA) iveliswa yi-prostate glandular kunye neeseli ze-endothelial. Litsheyina elinye le-glycoprotein elinobunzima bemolekyuli obumalunga nama-34 kDa.1 I-PSA ikhona kwiifom ezintathu eziphambili ezijikeleza kwiserum. Ezi fom zikhululekile i-PSA, i-PSA ibophelelwe kwi-α1 - i-Antichymotrypsin (i-PSA - ACT) kunye ne-PSA ehlanganiswe ne-α2-macroglobulin (PSA - MG) ng/mL.Inokuthi iphakanyiswe kwiimeko ezinobungozi ezifana nomhlaza wesifo seprotate, kwaye kwimeko enobungozi njenge-benign prostatic hyperplasia kunye ne-prostatitis.Inqanaba le-PSA le-3 ukuya kwi-10ng / ml lithathwa ngokuba "kwi-gray-zone" kunye namanqanaba angaphezu kwe-10ng / ml abonisa kakhulu umhlaza. nge-biopsy.

Uvavanyo lwe-prostate oluthile lwe-antigen sesona sixhobo sixabisekileyo esifumanekayo sokuxilongwa komhlaza we-prostate ekuqaleni.Izifundo ezininzi ziqinisekisile ukuba ubukho be-PSA yeyona nto iluncedo kwaye inentsingiselo ye-tumor marker eyaziwayo ngomhlaza we-prostate kunye nosulelo lwe-prostate lwe-Benign Prostatic Hyperplasia (BPH) .4) .

Isixhobo soVavanyo oluKhawulezayo lwe-PSA (igazi elipheleleyo/iSerum/iPlasma) sisebenzisa indibanisela ye-colloidal conjugate yegolide kunye ne-anti-PSA antibodies ukufumanisa ngokukhethekayo iyonke iPSA egazini, kwiserum okanye kwiplasma. Uvavanyo lunexabiso le-cut-off ye-3ng / ml kunye nexabiso lereferensi ye-10ng / ml.

【IZIKHOKELO ZOKUSETYENZISWA】

Vumela uvavanyo, umfuziselo, isithinteli kunye/okanye ulawulo ukufikelela kubushushu begumbi (15-30°C) phambi kovavanyo.

1. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokusivula.Susa isixhobo sovavanyo kwisingxobo esitywiniweyo kwaye usisebenzise ngokukhawuleza. Jija ithebhu ye-buffer vial ngaphandle kokuyicudisa.

2. Beka isixhobo kwindawo ecocekileyo kunye nenqanaba.

KubaISerum,Iisampulu zePlasma:

Bamba i-dropper ngokuthe nkqo kwaye udlulisele i-1 drop ye-serum okanye i-plasma (malunga ne-40uL) kwi-specimen well (S) yesixhobo sokuvavanya, uze udibanise i-1 drop of buffer (malunga ne-40uL) kwaye uqalise isibali-xesha.

KubaVenipuncture Igazi Elipheleleyo umzekelo:

Gcwalisa ityhubhu ye-capillary kunye namathontsi e-2 egazi elipheleleyo le-venipuncture (malunga ne-80uL) isampuli kwindawo yomzekelo wesixhobo sovavanyo, emva koko wongeze i-1 drop of buffer (malunga ne-40uL) kwaye uqalise isibali-xesha.

KubaUmnwe weGazi Elipheleleyo:

Ukusebenzisa ityhubhu ye-capillary:Gcwalisa ityhubhu ye-capillary kwaye udlulisele amathontsi ama-2 omfanekiso wegazi opheleleyo weminwe (malunga ne-80uL) kwindawo yomzekelo wesixhobo sovavanyo, uze wongeze ithontsi enye ye-buffer (malunga ne-40uL) kwaye uqalise isibali-xesha.

3. Lindela u(imi)layini onemibala ukuba ivele*.Funda iziphumo ngemizuzu eyi-15.Musa ukutolika umphumo emva kwemizuzu engama-20.