【ZVIRI KUSHANDISA】

The RSV Rapid Test Device in vitro diagnostic test for the qualitative discovery of Respiratory Syncytial Virus antigens muNasal Swab uye nasal aspirate samples, uchishandisa kukurumidza immunochromatographic method.Kuzivikanwa kunobva pa monoclonal antibodies zvakanangana neF protein yeRespiratory Syncytial Virus. Ichapa ruzivo kune vanachiremba vekiriniki kuti vanyore mishonga chaiyo. Migumisiro yakaipa inofanira kusimbiswa nedzimwe nzira, dzakadai sesero tsika.

【SUMMARY】

RSV is a negative single-stranded RNA virus yemhuri yeParamyxoviridae1. Ndiko honzeri yechirwere chinotapuriranwa zvakanyanya, chakakomba, chehutachiwana chekufema. Munyika yose, RSV inobatsira kufa kwevacheche nevana vari pasi pemakore mashanu gore rega rega2. Ichowo ndicho chikonzero chikuru chehutachiwana chechirwere che nosocomial muvana vagara vari muchipatara kune zvimwe zvikonzero3. Pakati pevana vari muchipatara vane chirwere cheRSV, nhamba yekufa inofungidzirwa yakaderera se0.3% kusvika 1.0% 4 uye pakati pe2.5% kusvika 4.0% yevana vane chirwere chemwoyo kana chemapapu5. Utachiona hweRSV hunongopa dziviriro shoma. Saka, vanhu vanokwanisa kuramba vachitapukirwa uye kuita zvirwere zvakakomba muhupenyu hwavo hwose6.

RSV ine serotype imwe chete, uye yakakamurwa kuva maviri antigenic subgroups, A uye B7. Mapoka ose ari maviri anotenderera panguva imwe chete munharaunda. Subtype B inotaridzwa seasymptomatic mhando dzehutachiona dzinosangana neruzhinji rwevanhu. Zvirwere zvekiriniki zvakanyanya zvinosanganisira subtype A strains, izvo zvinowanzoitika mukubuda kwakawanda. RSV inogona kufungidzirwa zvichienderana nenguva yegore yehutachiona; kuwanda kunowanzoenderana nemwaka wefuruwenza yechando.

RSV inogona kuongororwa murabhoritari pachishandiswa kufema.Kunzwa kwehutachiwana kuparadzaniswa kubva pakufema mumasero etsika kunosiyana pakati pemarabhoritari.Nyedzo dzeRT-PCR dzepamusoro dzinofanira kutariswa pakuyedzwa vanhu vakuru,nekuti vanogona kunge vaine hutachiwana hushoma mumienzaniso yavo yekufema. Kunyangwe zvichibatsira pakutsvaga, serologic bvunzo haiwanzo shandiswa kuongororwa. Seroconversion haina kuitika kweinenge 2 mavhiki uye ingada 4 ~ 6 mavhiki. Mazhinji marabhoritari ekiriniki parizvino anoshandisa antigen bvunzo dzekuona. Miedzo yekuona maantigen uye tsika zvinowanzovimbika muvana vadiki asi zvisinganyanyi kubatsira muvana vakura nevakuru.

Iyo RSV Antigen Rapid Test Device iri nyore lateral flow immunoassay yekuona yakananga maantigen eRSV. Ichapa kuongororwa kwekufungidzira kwehutachiona hweRSV.

【MAZANO EKUSHANDISA】

Bvumira chishandiso chekuyedza, bvunzo uye buffer kuti ienzane nekamuri tembiricha (15-30°C) isati yaedzwa. Ndokumbira utarise kadhi remaitiro mune ino kit.

1) Bvisa mudziyo wekuyedza kubva muhomwe yakavharwa nguva pfupi isati yaedzwa uye worara pabhenji rebasa.

2) Isai muromo nesefa muchubhu yekubvisa sampuli zvakasimba.

3) Dzosera sampuli yekubvisa chubhu, uye wedzera madonhwe matatu (angangoita 100μl) yebvunzo sampuro nekudzvanya chubhu yemhinduro yakabviswa muhwindo remuenzaniso.

4) Verenga mhinduro pamaminetsi gumi nemashanu.