【UKUSETSHENZISWA OKUHLOSIWE】

I-RSV Rapid Test Device iwukuhlolwa kokuxilonga okungaphakathi kwe-vitro kokutholwa kwekhwalithi yama-antigens e-Respiratory Syncytial Virus ku-Nasal Swab kanye namasampula e-nasal aspirate, kusetshenziswa indlela ye-immunochromatographic esheshayo.Ukuhlonza kusekelwe kumasosha omzimba we-monoclonal aqondene nephrotheni ye-F ye-Respiratory Syncytial Virus. Izohlinzeka ngolwazi kodokotela basemtholampilo ukuze banikeze imithi efanele. Imiphumela engemihle kufanele iqinisekiswe ngezinye izindlela, njengesiko lamaseli.

【ISIFINYEZO】

I-RSV iyigciwane le-RNA elilodwa elinegethivu elilodwa lomndeni wakwaParamyxoviridae1. Iyimbangela yesifo esithathelwanayo, esiyingozi, esibangelwa amagciwane emgudwini wokuphefumula.Emhlabeni wonke, i-RSV inesandla ekufeni kwezinsana nezingane ezifika ku-1,000,000 ezineminyaka engaphansi kwemihlanu unyaka ngamunye2. Futhi kuyimbangela enkulu yegciwane lesifo se-nosocomial ezinganeni esezivele zilaliswe esibhedlela ngenxa yezinye izizathu3. Phakathi kwezingane ezilaliswe esibhedlela ezinokutheleleka kwe-RSV, izinga lokufa lilinganiselwa ukuthi liphansi ukusuka ku-0.3% kuya ku-1.0%4 kanye nobubanzi obungu-2.5% ukuya ku-4.0% kuzingane ezinesifo senhliziyo noma samaphaphu5. Ukutheleleka kwe-RSV kunikeza ukuzivikela okulinganiselwe kuphela. Ngakho, abantu bangatheleleka ngokuphindaphindiwe futhi babe nesifo esibucayi phakathi nokuphila kwabo konke6.

I-RSV ine-serotype eyodwa kuphela, futhi ihlukaniswe ngamaqembu amancane amabili e-antigenic, A kanye ne-B7.Womabili amaqembu ajikeleza kanyekanye emphakathini. I-Subtype B ibonakala njengezinhlobo zegciwane ezingenazimpawu ezitholwa iningi labantu. Izifo zomtholampilo ezinzima kakhulu zihlanganisa izinhlobo ze-subtype A, ezivame ukugqama ekuqubukeni okuningi. I-RSV ingase isolwe ngokusekelwe esikhathini sonyaka sokutheleleka; ukwanda ngokuvamile kuhambisana nesikhathi somkhuhlane wasebusika.

I-RSV ingahlolwa elebhu kusetshenziswa uketshezi lokuphefumula.Ukuzwela kokuhlukaniswa kwegciwane ekukhiqizweni kokuphefumula kusiko lamaseli kuyahlukahluka phakathi kwamalabhorethri.Ukuhlolwa okuzwela kakhulu kwe-RT-PCR kufanele kucatshangelwe lapho kuhlolwa abantu abadala, ngoba bangase babe nenani eliphansi legciwane ezibonelweni zabo zokuphefumula. Nakuba luwusizo ocwaningweni, ukuhlolwa kwe-serologic akuvamile ukusetshenziselwa ukuxilongwa. I-Seroconversion ayenzeki okungenani amaviki angu-2 futhi ingadinga amaviki angu-4 ~ 6. Amalabhorethri amaningi omtholampilo okwamanje asebenzisa ukuhlola kokutholwa kwe-antigen. Ukuhlolwa kokutholwa kwe-antigen kanye namasiko ngokuvamile athembekile ezinganeni ezincane kodwa awusizo kangako ezinganeni ezindala nakubantu abadala.

Idivayisi ye-RSV Antigen Rapid Test iyi-assay ye-lateral flow immunoassay elula yokutholwa okuqondile kwama-antigen e-RSV. Izohlinzeka ngokuxilonga okucatshangelwayo kokutheleleka kwe-RSV.

【IZINDLELA ZOKUSEBENZISA】

Vumela idivayisi yokuhlola, isampula yokuhlola nebhafa ukuthi ilingane nezinga lokushisa legumbi (15-30°C) ngaphambi kokuhlolwa. Sicela ubheke ikhadi lenqubo kule kit.

1) Khipha idivayisi yokuhlola esikhwameni esivaliwe ngaphambi nje kokuhlolwa bese ulala phansi ebhentshini lomsebenzi.

2) Faka umlomo wesihlungi eshubhuni yokukhipha isampula ngokuqinile.

3) Hlehlisa ishubhu yokukhipha isampula, bese wengeza amaconsi ama-3 (cishe u-100μl) wesampula yokuhlola ngokucindezela ishubhu lesixazululo elikhishiwe efasiteleni lesampula.

4) Funda umphumela ngemizuzu eyi-15.